Papanicolaou and Kruger assessment of sperm morphology: Thresholds and agreement
The current World Health Organization guidelines suggest that the presence of ≥ 30% normal sperm forms (i.e. PAP criteria) is consistent with normal semen quality. Critical evaluation of sperm morphology (CE; Kruger classification) has shown an excellent correlation with human in vitro fertilization...
Guardado en:
| Publicado: |
1998
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01056263_v21_n6_p327_VazquezLevin http://hdl.handle.net/20.500.12110/paper_01056263_v21_n6_p327_VazquezLevin |
| Aporte de: |
| id |
paper:paper_01056263_v21_n6_p327_VazquezLevin |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_01056263_v21_n6_p327_VazquezLevin2023-06-08T15:10:33Z Papanicolaou and Kruger assessment of sperm morphology: Thresholds and agreement Kruger classification Sperm morphology WHO criteria article cell structure classification comparative study evaluation fertilization in vitro human human cell major clinical study male Papanicolaou test practice guideline priority journal retrospective study sperm statistical analysis world health organization Humans Male Predictive Value of Tests Retrospective Studies Sensitivity and Specificity Spermatozoa The current World Health Organization guidelines suggest that the presence of ≥ 30% normal sperm forms (i.e. PAP criteria) is consistent with normal semen quality. Critical evaluation of sperm morphology (CE; Kruger classification) has shown an excellent correlation with human in vitro fertilization. Utilizing Kruger criteria, > 14% normal sperm forms has been proposed as indicative of normal semen quality. We have performed a retrospective analysis on 261 individuals to assess the agreement between PAP and Kruger criteria for normal sperm morphology (NSM). When the threshold for NSM by PAP was set at 30%, a significant agreement was found between the percentage normal forms of both criteria (Kappa coefficient = 0.37; p < 0.001). Sixty-seven (92%) of the 73 men found to have abnormal sperm morphology by PAP had abnormal semen by Kruger classification. When the threshold for NSM by PAP was established at 50%, the Kappa coefficient was 0.48 (p < 0.001). Sixty of the 72 samples (83%) classified as normal by PAP staining were normal by Kruger criteria. Interestingly, when NSM by PAP was between 30 and 50%, the specimen was just as likely to have normal or abnormal sperm morphology by Kruger (40 vs. 60%, respectively). These results strongly suggest that a high or low percentage of NSM by PAP is in agreement with the Kruger classification. The excellent agreement of Kruger and WHO criteria at the extremes (< 30% and > 50%) may obviate the need for Kruger assessment. However, when WHO morphology is between 30 and 50%, the addition of Kruger evaluation may provide meaningful information to help better diagnose a patient and plan his treatment. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01056263_v21_n6_p327_VazquezLevin http://hdl.handle.net/20.500.12110/paper_01056263_v21_n6_p327_VazquezLevin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Kruger classification Sperm morphology WHO criteria article cell structure classification comparative study evaluation fertilization in vitro human human cell major clinical study male Papanicolaou test practice guideline priority journal retrospective study sperm statistical analysis world health organization Humans Male Predictive Value of Tests Retrospective Studies Sensitivity and Specificity Spermatozoa |
| spellingShingle |
Kruger classification Sperm morphology WHO criteria article cell structure classification comparative study evaluation fertilization in vitro human human cell major clinical study male Papanicolaou test practice guideline priority journal retrospective study sperm statistical analysis world health organization Humans Male Predictive Value of Tests Retrospective Studies Sensitivity and Specificity Spermatozoa Papanicolaou and Kruger assessment of sperm morphology: Thresholds and agreement |
| topic_facet |
Kruger classification Sperm morphology WHO criteria article cell structure classification comparative study evaluation fertilization in vitro human human cell major clinical study male Papanicolaou test practice guideline priority journal retrospective study sperm statistical analysis world health organization Humans Male Predictive Value of Tests Retrospective Studies Sensitivity and Specificity Spermatozoa |
| description |
The current World Health Organization guidelines suggest that the presence of ≥ 30% normal sperm forms (i.e. PAP criteria) is consistent with normal semen quality. Critical evaluation of sperm morphology (CE; Kruger classification) has shown an excellent correlation with human in vitro fertilization. Utilizing Kruger criteria, > 14% normal sperm forms has been proposed as indicative of normal semen quality. We have performed a retrospective analysis on 261 individuals to assess the agreement between PAP and Kruger criteria for normal sperm morphology (NSM). When the threshold for NSM by PAP was set at 30%, a significant agreement was found between the percentage normal forms of both criteria (Kappa coefficient = 0.37; p < 0.001). Sixty-seven (92%) of the 73 men found to have abnormal sperm morphology by PAP had abnormal semen by Kruger classification. When the threshold for NSM by PAP was established at 50%, the Kappa coefficient was 0.48 (p < 0.001). Sixty of the 72 samples (83%) classified as normal by PAP staining were normal by Kruger criteria. Interestingly, when NSM by PAP was between 30 and 50%, the specimen was just as likely to have normal or abnormal sperm morphology by Kruger (40 vs. 60%, respectively). These results strongly suggest that a high or low percentage of NSM by PAP is in agreement with the Kruger classification. The excellent agreement of Kruger and WHO criteria at the extremes (< 30% and > 50%) may obviate the need for Kruger assessment. However, when WHO morphology is between 30 and 50%, the addition of Kruger evaluation may provide meaningful information to help better diagnose a patient and plan his treatment. |
| title |
Papanicolaou and Kruger assessment of sperm morphology: Thresholds and agreement |
| title_short |
Papanicolaou and Kruger assessment of sperm morphology: Thresholds and agreement |
| title_full |
Papanicolaou and Kruger assessment of sperm morphology: Thresholds and agreement |
| title_fullStr |
Papanicolaou and Kruger assessment of sperm morphology: Thresholds and agreement |
| title_full_unstemmed |
Papanicolaou and Kruger assessment of sperm morphology: Thresholds and agreement |
| title_sort |
papanicolaou and kruger assessment of sperm morphology: thresholds and agreement |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01056263_v21_n6_p327_VazquezLevin http://hdl.handle.net/20.500.12110/paper_01056263_v21_n6_p327_VazquezLevin |
| _version_ |
1768542932964474880 |