The CreC regulator of Escherichia coli, a new target for metabolic manipulations
The CreBC (carbon source-responsive) two-component regulation system of Escherichia coli affects a number of functions, including intermediary carbon catabolism. The impacts of different creC mutations (a ΔcreC mutant and a mutant carrying the constitutive creC510 allele) on bacterial physiology wer...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v82_n1_p244_Godoy http://hdl.handle.net/20.500.12110/paper_00992240_v82_n1_p244_Godoy |
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paper:paper_00992240_v82_n1_p244_Godoy2023-06-08T15:10:09Z The CreC regulator of Escherichia coli, a new target for metabolic manipulations Enzyme activity Escherichia coli Glucose Metabolism Oxygen Biochemical network Engineering manipulations Extracellular metabolites Formate dehydrogenase Metabolic changes Metabolic effects Phosphoenolpyruvate carboxylase Wild-type strain Metabolic engineering acetate aeration catabolism coliform bacterium metabolism microbial community physiology Bacteria (microorganisms) Candida boidinii Escherichia coli CreC protein, E coli Escherichia coli protein glucose nicotinamide adenine dinucleotide oxygen protein kinase reduced nicotinamide adenine dinucleotide dehydrogenase succinic acid anaerobic growth Escherichia coli gene expression regulation genetics metabolic engineering metabolism mutation oxidation reduction reaction protein engineering Anaerobiosis Escherichia coli Escherichia coli Proteins Gene Expression Regulation, Bacterial Glucose Metabolic Engineering Mutation NAD NADH Dehydrogenase Oxidation-Reduction Oxygen Protein Engineering Protein Kinases Succinic Acid The CreBC (carbon source-responsive) two-component regulation system of Escherichia coli affects a number of functions, including intermediary carbon catabolism. The impacts of different creC mutations (a ΔcreC mutant and a mutant carrying the constitutive creC510 allele) on bacterial physiology were analyzed in glucose cultures under three oxygen availability conditions. Differences in the amounts of extracellular metabolites produced were observed in the null mutant compared to the wild-type strain and the mutant carrying creC510 and shown to be affected by oxygen availability. The ΔcreC strain secreted more formate, succinate, and acetate but less lactate under low aeration. These metabolic changes were associated with differences in AckA and LdhA activities, both of which were affected by CreC. Measurement of the NAD(P)H/NAD(P)+ ratios showed that the creC510 strain had a more reduced intracellular redox state, while the opposite was observed for the ΔcreC mutant, particularly under intermediate oxygen availability conditions, indicating that CreC affects redox balance. The null mutant formed more succinate than the wild-type strain under both low aeration and no aeration. Overexpression of the genes encoding phosphoenolpyruvate carboxylase from E. coli and a NADH-forming formate dehydrogenase from Candida boidinii in the ΔcreC mutant further increased the yield of succinate on glucose. Interestingly, the elimination of ackA and adhE did not significantly improve the production of succinate. The diverse metabolic effects of this regulator on the central biochemical network of E. coli make it a good candidate for metabolic-engineering manipulations to enhance the formation of bioproducts, such as succinate. © 2015, American Society for Microbiology. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v82_n1_p244_Godoy http://hdl.handle.net/20.500.12110/paper_00992240_v82_n1_p244_Godoy |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Enzyme activity Escherichia coli Glucose Metabolism Oxygen Biochemical network Engineering manipulations Extracellular metabolites Formate dehydrogenase Metabolic changes Metabolic effects Phosphoenolpyruvate carboxylase Wild-type strain Metabolic engineering acetate aeration catabolism coliform bacterium metabolism microbial community physiology Bacteria (microorganisms) Candida boidinii Escherichia coli CreC protein, E coli Escherichia coli protein glucose nicotinamide adenine dinucleotide oxygen protein kinase reduced nicotinamide adenine dinucleotide dehydrogenase succinic acid anaerobic growth Escherichia coli gene expression regulation genetics metabolic engineering metabolism mutation oxidation reduction reaction protein engineering Anaerobiosis Escherichia coli Escherichia coli Proteins Gene Expression Regulation, Bacterial Glucose Metabolic Engineering Mutation NAD NADH Dehydrogenase Oxidation-Reduction Oxygen Protein Engineering Protein Kinases Succinic Acid |
| spellingShingle |
Enzyme activity Escherichia coli Glucose Metabolism Oxygen Biochemical network Engineering manipulations Extracellular metabolites Formate dehydrogenase Metabolic changes Metabolic effects Phosphoenolpyruvate carboxylase Wild-type strain Metabolic engineering acetate aeration catabolism coliform bacterium metabolism microbial community physiology Bacteria (microorganisms) Candida boidinii Escherichia coli CreC protein, E coli Escherichia coli protein glucose nicotinamide adenine dinucleotide oxygen protein kinase reduced nicotinamide adenine dinucleotide dehydrogenase succinic acid anaerobic growth Escherichia coli gene expression regulation genetics metabolic engineering metabolism mutation oxidation reduction reaction protein engineering Anaerobiosis Escherichia coli Escherichia coli Proteins Gene Expression Regulation, Bacterial Glucose Metabolic Engineering Mutation NAD NADH Dehydrogenase Oxidation-Reduction Oxygen Protein Engineering Protein Kinases Succinic Acid The CreC regulator of Escherichia coli, a new target for metabolic manipulations |
| topic_facet |
Enzyme activity Escherichia coli Glucose Metabolism Oxygen Biochemical network Engineering manipulations Extracellular metabolites Formate dehydrogenase Metabolic changes Metabolic effects Phosphoenolpyruvate carboxylase Wild-type strain Metabolic engineering acetate aeration catabolism coliform bacterium metabolism microbial community physiology Bacteria (microorganisms) Candida boidinii Escherichia coli CreC protein, E coli Escherichia coli protein glucose nicotinamide adenine dinucleotide oxygen protein kinase reduced nicotinamide adenine dinucleotide dehydrogenase succinic acid anaerobic growth Escherichia coli gene expression regulation genetics metabolic engineering metabolism mutation oxidation reduction reaction protein engineering Anaerobiosis Escherichia coli Escherichia coli Proteins Gene Expression Regulation, Bacterial Glucose Metabolic Engineering Mutation NAD NADH Dehydrogenase Oxidation-Reduction Oxygen Protein Engineering Protein Kinases Succinic Acid |
| description |
The CreBC (carbon source-responsive) two-component regulation system of Escherichia coli affects a number of functions, including intermediary carbon catabolism. The impacts of different creC mutations (a ΔcreC mutant and a mutant carrying the constitutive creC510 allele) on bacterial physiology were analyzed in glucose cultures under three oxygen availability conditions. Differences in the amounts of extracellular metabolites produced were observed in the null mutant compared to the wild-type strain and the mutant carrying creC510 and shown to be affected by oxygen availability. The ΔcreC strain secreted more formate, succinate, and acetate but less lactate under low aeration. These metabolic changes were associated with differences in AckA and LdhA activities, both of which were affected by CreC. Measurement of the NAD(P)H/NAD(P)+ ratios showed that the creC510 strain had a more reduced intracellular redox state, while the opposite was observed for the ΔcreC mutant, particularly under intermediate oxygen availability conditions, indicating that CreC affects redox balance. The null mutant formed more succinate than the wild-type strain under both low aeration and no aeration. Overexpression of the genes encoding phosphoenolpyruvate carboxylase from E. coli and a NADH-forming formate dehydrogenase from Candida boidinii in the ΔcreC mutant further increased the yield of succinate on glucose. Interestingly, the elimination of ackA and adhE did not significantly improve the production of succinate. The diverse metabolic effects of this regulator on the central biochemical network of E. coli make it a good candidate for metabolic-engineering manipulations to enhance the formation of bioproducts, such as succinate. © 2015, American Society for Microbiology. |
| title |
The CreC regulator of Escherichia coli, a new target for metabolic manipulations |
| title_short |
The CreC regulator of Escherichia coli, a new target for metabolic manipulations |
| title_full |
The CreC regulator of Escherichia coli, a new target for metabolic manipulations |
| title_fullStr |
The CreC regulator of Escherichia coli, a new target for metabolic manipulations |
| title_full_unstemmed |
The CreC regulator of Escherichia coli, a new target for metabolic manipulations |
| title_sort |
crec regulator of escherichia coli, a new target for metabolic manipulations |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v82_n1_p244_Godoy http://hdl.handle.net/20.500.12110/paper_00992240_v82_n1_p244_Godoy |
| _version_ |
1768541885274521600 |