Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli

Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB f...

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Autores principales:	de Almeida, Alejandra, Pettinari, María Julia
Publicado:	2010
Materias:	Agitation conditions Batch culture Bioreactor cultures Poly(3-hydroxybutyrate) Recombinant Escherichia coli Relative molecular mass Batch cell culture Cell culture Escherichia coli Ethanol Glycerol Glucose alcohol carboxylic acid glucose glycerol hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester recombinant protein aeration bioreactor coliform bacterium ester recombination aerobic metabolism article Azotobacter chemistry fermentation genetics metabolism molecular weight Aerobiosis Bioreactors Carboxylic Acids Fermentation Hydroxybutyrates Molecular Weight Polyesters Recombinant Proteins Bacteria (microorganisms)
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v76_n6_p2036_DeAlmeida http://hdl.handle.net/20.500.12110/paper_00992240_v76_n6_p2036_DeAlmeida
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00992240_v76_n6_p2036_DeAlmeida
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spelling	paper:paper_00992240_v76_n6_p2036_DeAlmeida2023-06-08T15:10:06Z Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli de Almeida, Alejandra Pettinari, María Julia Agitation conditions Batch culture Bioreactor cultures Poly(3-hydroxybutyrate) Recombinant Escherichia coli Relative molecular mass Batch cell culture Cell culture Escherichia coli Ethanol Glycerol Glucose alcohol carboxylic acid glucose glycerol hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester recombinant protein aeration alcohol bioreactor coliform bacterium ester glucose recombination aerobic metabolism article Azotobacter bioreactor chemistry Escherichia coli fermentation genetics metabolism molecular weight Aerobiosis Azotobacter Bioreactors Carboxylic Acids Escherichia coli Ethanol Fermentation Glucose Glycerol Hydroxybutyrates Molecular Weight Polyesters Recombinant Proteins Azotobacter Bacteria (microorganisms) Escherichia coli Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation. Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose. Copyright © 2010, American Society for Microbiology. All Rights Reserved. Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v76_n6_p2036_DeAlmeida http://hdl.handle.net/20.500.12110/paper_00992240_v76_n6_p2036_DeAlmeida
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Agitation conditions Batch culture Bioreactor cultures Poly(3-hydroxybutyrate) Recombinant Escherichia coli Relative molecular mass Batch cell culture Cell culture Escherichia coli Ethanol Glycerol Glucose alcohol carboxylic acid glucose glycerol hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester recombinant protein aeration alcohol bioreactor coliform bacterium ester glucose recombination aerobic metabolism article Azotobacter bioreactor chemistry Escherichia coli fermentation genetics metabolism molecular weight Aerobiosis Azotobacter Bioreactors Carboxylic Acids Escherichia coli Ethanol Fermentation Glucose Glycerol Hydroxybutyrates Molecular Weight Polyesters Recombinant Proteins Azotobacter Bacteria (microorganisms) Escherichia coli
spellingShingle	Agitation conditions Batch culture Bioreactor cultures Poly(3-hydroxybutyrate) Recombinant Escherichia coli Relative molecular mass Batch cell culture Cell culture Escherichia coli Ethanol Glycerol Glucose alcohol carboxylic acid glucose glycerol hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester recombinant protein aeration alcohol bioreactor coliform bacterium ester glucose recombination aerobic metabolism article Azotobacter bioreactor chemistry Escherichia coli fermentation genetics metabolism molecular weight Aerobiosis Azotobacter Bioreactors Carboxylic Acids Escherichia coli Ethanol Fermentation Glucose Glycerol Hydroxybutyrates Molecular Weight Polyesters Recombinant Proteins Azotobacter Bacteria (microorganisms) Escherichia coli de Almeida, Alejandra Pettinari, María Julia Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
topic_facet	Agitation conditions Batch culture Bioreactor cultures Poly(3-hydroxybutyrate) Recombinant Escherichia coli Relative molecular mass Batch cell culture Cell culture Escherichia coli Ethanol Glycerol Glucose alcohol carboxylic acid glucose glycerol hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester recombinant protein aeration alcohol bioreactor coliform bacterium ester glucose recombination aerobic metabolism article Azotobacter bioreactor chemistry Escherichia coli fermentation genetics metabolism molecular weight Aerobiosis Azotobacter Bioreactors Carboxylic Acids Escherichia coli Ethanol Fermentation Glucose Glycerol Hydroxybutyrates Molecular Weight Polyesters Recombinant Proteins Azotobacter Bacteria (microorganisms) Escherichia coli
description	Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation. Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
author	de Almeida, Alejandra Pettinari, María Julia
author_facet	de Almeida, Alejandra Pettinari, María Julia
author_sort	de Almeida, Alejandra
title	Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
title_short	Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
title_full	Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
title_fullStr	Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
title_full_unstemmed	Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
title_sort	effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant escherichia coli
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v76_n6_p2036_DeAlmeida http://hdl.handle.net/20.500.12110/paper_00992240_v76_n6_p2036_DeAlmeida
work_keys_str_mv	AT dealmeidaalejandra effectsofaerationonthesynthesisofpoly3hydroxybutyratefromglycerolandglucoseinrecombinantescherichiacoli AT pettinarimariajulia effectsofaerationonthesynthesisofpoly3hydroxybutyratefromglycerolandglucoseinrecombinantescherichiacoli
_version_	1768541744842932224

Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli

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