Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth

We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathoge...

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Autores principales:	Prado Acosta, Mariano, Ruzal, Sandra Mónica, Allievi, Mariana Claudia, Palomino, María Mercedes
Publicado:	2010
Materias:	Bacilli Bacillus cereus Bacteriology Cytology Hydrolases Salmonella Bacterial growth Gram-positive bacterium Hydrolase activities Lactobacillus acidophilus Proton-motive forces Salmonella enterica Staphylococcus aureus Synergistic effect Antibiotics bacterium enzyme activity growth rate membrane surface layer synergism Bacteria (microorganisms) Negibacteria Posibacteria
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v76_n3_p974_PradoAcosta http://hdl.handle.net/20.500.12110/paper_00992240_v76_n3_p974_PradoAcosta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00992240_v76_n3_p974_PradoAcosta
record_format	dspace
spelling	paper:paper_00992240_v76_n3_p974_PradoAcosta2023-06-08T15:10:06Z Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth Prado Acosta, Mariano Ruzal, Sandra Mónica Allievi, Mariana Claudia Palomino, María Mercedes Bacilli Bacillus cereus Bacteriology Cytology Hydrolases Salmonella Bacterial growth Gram-positive bacterium Hydrolase activities Lactobacillus acidophilus Proton-motive forces Salmonella enterica Staphylococcus aureus Synergistic effect Antibiotics bacterium enzyme activity growth rate membrane surface layer synergism Bacillus cereus Bacteria (microorganisms) Lactobacillus acidophilus Negibacteria Posibacteria Salmonella enterica Staphylococcus aureus We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved. Fil:Prado-Acosta, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v76_n3_p974_PradoAcosta http://hdl.handle.net/20.500.12110/paper_00992240_v76_n3_p974_PradoAcosta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacilli Bacillus cereus Bacteriology Cytology Hydrolases Salmonella Bacterial growth Gram-positive bacterium Hydrolase activities Lactobacillus acidophilus Proton-motive forces Salmonella enterica Staphylococcus aureus Synergistic effect Antibiotics bacterium enzyme activity growth rate membrane surface layer synergism Bacillus cereus Bacteria (microorganisms) Lactobacillus acidophilus Negibacteria Posibacteria Salmonella enterica Staphylococcus aureus
spellingShingle	Bacilli Bacillus cereus Bacteriology Cytology Hydrolases Salmonella Bacterial growth Gram-positive bacterium Hydrolase activities Lactobacillus acidophilus Proton-motive forces Salmonella enterica Staphylococcus aureus Synergistic effect Antibiotics bacterium enzyme activity growth rate membrane surface layer synergism Bacillus cereus Bacteria (microorganisms) Lactobacillus acidophilus Negibacteria Posibacteria Salmonella enterica Staphylococcus aureus Prado Acosta, Mariano Ruzal, Sandra Mónica Allievi, Mariana Claudia Palomino, María Mercedes Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
topic_facet	Bacilli Bacillus cereus Bacteriology Cytology Hydrolases Salmonella Bacterial growth Gram-positive bacterium Hydrolase activities Lactobacillus acidophilus Proton-motive forces Salmonella enterica Staphylococcus aureus Synergistic effect Antibiotics bacterium enzyme activity growth rate membrane surface layer synergism Bacillus cereus Bacteria (microorganisms) Lactobacillus acidophilus Negibacteria Posibacteria Salmonella enterica Staphylococcus aureus
description	We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
author	Prado Acosta, Mariano Ruzal, Sandra Mónica Allievi, Mariana Claudia Palomino, María Mercedes
author_facet	Prado Acosta, Mariano Ruzal, Sandra Mónica Allievi, Mariana Claudia Palomino, María Mercedes
author_sort	Prado Acosta, Mariano
title	Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_short	Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_full	Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_fullStr	Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_full_unstemmed	Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_sort	synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v76_n3_p974_PradoAcosta http://hdl.handle.net/20.500.12110/paper_00992240_v76_n3_p974_PradoAcosta
work_keys_str_mv	AT pradoacostamariano synergisticeffectsofthelactobacillusacidophilussurfacelayerandnisinonbacterialgrowth AT ruzalsandramonica synergisticeffectsofthelactobacillusacidophilussurfacelayerandnisinonbacterialgrowth AT allievimarianaclaudia synergisticeffectsofthelactobacillusacidophilussurfacelayerandnisinonbacterialgrowth AT palominomariamercedes synergisticeffectsofthelactobacillusacidophilussurfacelayerandnisinonbacterialgrowth
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Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth

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