Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8

Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene...

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Autores principales:	Pettinari, María Julia, Vazquez, Gustavo, Silberschmidt, Daniel, Méndez, Beatriz Silvia
Publicado:	2001
Materias:	acetoacetyl coenzyme a reductase acetoacetyl-CoA reductase acetyl coenzyme A acyltransferase acyltransferase alcohol dehydrogenase hydroxybutyric acid poly beta hydroxybutyrate polymerase poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate poly-beta-hydroxybutyrate polymerase polyester article Azotobacter bacterial gene DNA sequence gene deletion genetics growth, development and aging metabolism molecular cloning molecular genetics Acetyl-CoA C-Acyltransferase Acyltransferases Alcohol Oxidoreductases Cloning, Molecular Gene Deletion Genes, Bacterial Hydroxybutyrates Molecular Sequence Data Polyesters Sequence Analysis, DNA
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v67_n3-12_p5331_Pettinari http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3-12_p5331_Pettinari
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00992240_v67_n3-12_p5331_Pettinari
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spelling	paper:paper_00992240_v67_n3-12_p5331_Pettinari2023-06-08T15:10:03Z Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8 Pettinari, María Julia Vazquez, Gustavo Silberschmidt, Daniel Méndez, Beatriz Silvia acetoacetyl coenzyme a reductase acetoacetyl-CoA reductase acetyl coenzyme A acyltransferase acyltransferase alcohol dehydrogenase hydroxybutyric acid poly beta hydroxybutyrate polymerase poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate poly-beta-hydroxybutyrate polymerase polyester article Azotobacter bacterial gene DNA sequence gene deletion genetics growth, development and aging metabolism molecular cloning molecular genetics Acetyl-CoA C-Acyltransferase Acyltransferases Alcohol Oxidoreductases Azotobacter Cloning, Molecular Gene Deletion Genes, Bacterial Hydroxybutyrates Molecular Sequence Data Polyesters Sequence Analysis, DNA Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Raktonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or oclanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chuin-length hydroxyalkanoates into PHB. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vázquez, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Silberschmidt, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v67_n3-12_p5331_Pettinari http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3-12_p5331_Pettinari
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acetoacetyl coenzyme a reductase acetoacetyl-CoA reductase acetyl coenzyme A acyltransferase acyltransferase alcohol dehydrogenase hydroxybutyric acid poly beta hydroxybutyrate polymerase poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate poly-beta-hydroxybutyrate polymerase polyester article Azotobacter bacterial gene DNA sequence gene deletion genetics growth, development and aging metabolism molecular cloning molecular genetics Acetyl-CoA C-Acyltransferase Acyltransferases Alcohol Oxidoreductases Azotobacter Cloning, Molecular Gene Deletion Genes, Bacterial Hydroxybutyrates Molecular Sequence Data Polyesters Sequence Analysis, DNA
spellingShingle	acetoacetyl coenzyme a reductase acetoacetyl-CoA reductase acetyl coenzyme A acyltransferase acyltransferase alcohol dehydrogenase hydroxybutyric acid poly beta hydroxybutyrate polymerase poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate poly-beta-hydroxybutyrate polymerase polyester article Azotobacter bacterial gene DNA sequence gene deletion genetics growth, development and aging metabolism molecular cloning molecular genetics Acetyl-CoA C-Acyltransferase Acyltransferases Alcohol Oxidoreductases Azotobacter Cloning, Molecular Gene Deletion Genes, Bacterial Hydroxybutyrates Molecular Sequence Data Polyesters Sequence Analysis, DNA Pettinari, María Julia Vazquez, Gustavo Silberschmidt, Daniel Méndez, Beatriz Silvia Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
topic_facet	acetoacetyl coenzyme a reductase acetoacetyl-CoA reductase acetyl coenzyme A acyltransferase acyltransferase alcohol dehydrogenase hydroxybutyric acid poly beta hydroxybutyrate polymerase poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate poly-beta-hydroxybutyrate polymerase polyester article Azotobacter bacterial gene DNA sequence gene deletion genetics growth, development and aging metabolism molecular cloning molecular genetics Acetyl-CoA C-Acyltransferase Acyltransferases Alcohol Oxidoreductases Azotobacter Cloning, Molecular Gene Deletion Genes, Bacterial Hydroxybutyrates Molecular Sequence Data Polyesters Sequence Analysis, DNA
description	Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Raktonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or oclanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chuin-length hydroxyalkanoates into PHB.
author	Pettinari, María Julia Vazquez, Gustavo Silberschmidt, Daniel Méndez, Beatriz Silvia
author_facet	Pettinari, María Julia Vazquez, Gustavo Silberschmidt, Daniel Méndez, Beatriz Silvia
author_sort	Pettinari, María Julia
title	Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
title_short	Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
title_full	Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
title_fullStr	Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
title_full_unstemmed	Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
title_sort	poly(3-hydroxybutyrate) synthesis genes in azotobacter sp. strain fa8
publishDate	2001
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v67_n3-12_p5331_Pettinari http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3-12_p5331_Pettinari
work_keys_str_mv	AT pettinarimariajulia poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8 AT vazquezgustavo poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8 AT silberschmidtdaniel poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8 AT mendezbeatrizsilvia poly3hydroxybutyratesynthesisgenesinazotobacterspstrainfa8
_version_	1768542214233784320

Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8

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