AFLP characterization of three argentine Coprotus species

AFLP methodology was applied to characterize three Coprotus species (C. lacteus, C. niveus, C. sexdecimsporus) so as to estimate the levels of polymorphism within species, to analyze the phenetic relationships among them, and to contrast the AFLP findings to those of a previous RAPD study. The high...

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Autores principales: Ramos, Araceli Marcela, Ferreyra, Laura Inés, Cinto, Isabel Esther
Publicado: 2015
Materias:
Ascomycota
Coprophilous
Molecular markers
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v130_n1_p265_Ramos
http://hdl.handle.net/20.500.12110/paper_00934666_v130_n1_p265_Ramos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00934666_v130_n1_p265_Ramos
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spelling paper:paper_00934666_v130_n1_p265_Ramos2023-06-08T15:08:50Z AFLP characterization of three argentine Coprotus species Ramos, Araceli Marcela Ferreyra, Laura Inés Cinto, Isabel Esther Ascomycota Coprophilous Molecular markers AFLP methodology was applied to characterize three Coprotus species (C. lacteus, C. niveus, C. sexdecimsporus) so as to estimate the levels of polymorphism within species, to analyze the phenetic relationships among them, and to contrast the AFLP findings to those of a previous RAPD study. The high number of AFLP bands obtained with the six assayed primers allowed us to detect intra-specific variability. The genetic variability within species obtained using AFLP (measured in terms of percentage of polymorphic loci) was two to three times higher than those obtained by RAPD. The phenograms generated by AFLP markers grouped all strains of the same species into three defined clusters, and a higher association between C. lacteus and C. sexdecimsporus was also observed. The AFLP technique could become a powerful tool for genera such as Coprotus, in which a high intra-specific homogeneity does not allow detection of genetic variability using other PCR-based markers. © 2015. Mycotaxon, Ltd. Fil:Ramos, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferreyra, L.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cinto, I.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v130_n1_p265_Ramos http://hdl.handle.net/20.500.12110/paper_00934666_v130_n1_p265_Ramos
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ascomycota
Coprophilous
Molecular markers
spellingShingle Ascomycota
Coprophilous
Molecular markers
Ramos, Araceli Marcela
Ferreyra, Laura Inés
Cinto, Isabel Esther
AFLP characterization of three argentine Coprotus species
topic_facet Ascomycota
Coprophilous
Molecular markers
description AFLP methodology was applied to characterize three Coprotus species (C. lacteus, C. niveus, C. sexdecimsporus) so as to estimate the levels of polymorphism within species, to analyze the phenetic relationships among them, and to contrast the AFLP findings to those of a previous RAPD study. The high number of AFLP bands obtained with the six assayed primers allowed us to detect intra-specific variability. The genetic variability within species obtained using AFLP (measured in terms of percentage of polymorphic loci) was two to three times higher than those obtained by RAPD. The phenograms generated by AFLP markers grouped all strains of the same species into three defined clusters, and a higher association between C. lacteus and C. sexdecimsporus was also observed. The AFLP technique could become a powerful tool for genera such as Coprotus, in which a high intra-specific homogeneity does not allow detection of genetic variability using other PCR-based markers. © 2015. Mycotaxon, Ltd.
author Ramos, Araceli Marcela
Ferreyra, Laura Inés
Cinto, Isabel Esther
author_facet Ramos, Araceli Marcela
Ferreyra, Laura Inés
Cinto, Isabel Esther
author_sort Ramos, Araceli Marcela
title AFLP characterization of three argentine Coprotus species
title_short AFLP characterization of three argentine Coprotus species
title_full AFLP characterization of three argentine Coprotus species
title_fullStr AFLP characterization of three argentine Coprotus species
title_full_unstemmed AFLP characterization of three argentine Coprotus species
title_sort aflp characterization of three argentine coprotus species
publishDate 2015
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v130_n1_p265_Ramos
http://hdl.handle.net/20.500.12110/paper_00934666_v130_n1_p265_Ramos
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