RAPD patterns in three Argentine Coprotus species: A test case

The genus Coprotus consists of coprophilous fungi in the Pyronemataceae. 44 monosporic strains were morphologically identified as Coprotus lacteus, C. niveus, and C. sexdecimsporus. Using random amplified polymorphic DNA (RAPD) to analyse two C. lacteus, five C. niveus, and two C. sexdecimsporus geo...

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Autores principales: Ramos, Araceli Marcela, Dokmetzian, Diana Ana, Ranalli de Cinto, María Esther, Ferreyra, Laura Inés
Publicado: 2008
Materias:
Ascomycota
Taxonomy
Coprophilous
Coprotus
Coprotus niveus
Fungi
Pyronemataceae
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v103_n_p9_Ramos
http://hdl.handle.net/20.500.12110/paper_00934666_v103_n_p9_Ramos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00934666_v103_n_p9_Ramos
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spelling paper:paper_00934666_v103_n_p9_Ramos2023-06-08T15:08:44Z RAPD patterns in three Argentine Coprotus species: A test case Ramos, Araceli Marcela Dokmetzian, Diana Ana Ranalli de Cinto, María Esther Ferreyra, Laura Inés Ascomycota Taxonomy Ascomycota Coprophilous Coprotus Coprotus niveus Fungi Pyronemataceae The genus Coprotus consists of coprophilous fungi in the Pyronemataceae. 44 monosporic strains were morphologically identified as Coprotus lacteus, C. niveus, and C. sexdecimsporus. Using random amplified polymorphic DNA (RAPD) to analyse two C. lacteus, five C. niveus, and two C. sexdecimsporus geographical groups, the authors obtained diagnostic bands for species recognition, estimated polymorphism levels within species, analysed inter-species phenetic relationships, and compared the findings from the RAPD study to those from a previous isozyme study. The phenogram obtained from Nei s genetic distances matrix was similar to the one previously obtained by isozyme markers. Both matrices show strains grouping into three defined clusters, each representing one of the three species analysed. The difference between the similarity levels among species and those observed in the isozyme report are compared and discussed. The results of this study support the use of RAPD markers as a reliable tool comparable to traditional morphological and isozyme methods. Fil:Ramos, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dokmetzian, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ranalli, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferreyra, L.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v103_n_p9_Ramos http://hdl.handle.net/20.500.12110/paper_00934666_v103_n_p9_Ramos
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ascomycota
Taxonomy
Ascomycota
Coprophilous
Coprotus
Coprotus niveus
Fungi
Pyronemataceae
spellingShingle Ascomycota
Taxonomy
Ascomycota
Coprophilous
Coprotus
Coprotus niveus
Fungi
Pyronemataceae
Ramos, Araceli Marcela
Dokmetzian, Diana Ana
Ranalli de Cinto, María Esther
Ferreyra, Laura Inés
RAPD patterns in three Argentine Coprotus species: A test case
topic_facet Ascomycota
Taxonomy
Ascomycota
Coprophilous
Coprotus
Coprotus niveus
Fungi
Pyronemataceae
description The genus Coprotus consists of coprophilous fungi in the Pyronemataceae. 44 monosporic strains were morphologically identified as Coprotus lacteus, C. niveus, and C. sexdecimsporus. Using random amplified polymorphic DNA (RAPD) to analyse two C. lacteus, five C. niveus, and two C. sexdecimsporus geographical groups, the authors obtained diagnostic bands for species recognition, estimated polymorphism levels within species, analysed inter-species phenetic relationships, and compared the findings from the RAPD study to those from a previous isozyme study. The phenogram obtained from Nei s genetic distances matrix was similar to the one previously obtained by isozyme markers. Both matrices show strains grouping into three defined clusters, each representing one of the three species analysed. The difference between the similarity levels among species and those observed in the isozyme report are compared and discussed. The results of this study support the use of RAPD markers as a reliable tool comparable to traditional morphological and isozyme methods.
author Ramos, Araceli Marcela
Dokmetzian, Diana Ana
Ranalli de Cinto, María Esther
Ferreyra, Laura Inés
author_facet Ramos, Araceli Marcela
Dokmetzian, Diana Ana
Ranalli de Cinto, María Esther
Ferreyra, Laura Inés
author_sort Ramos, Araceli Marcela
title RAPD patterns in three Argentine Coprotus species: A test case
title_short RAPD patterns in three Argentine Coprotus species: A test case
title_full RAPD patterns in three Argentine Coprotus species: A test case
title_fullStr RAPD patterns in three Argentine Coprotus species: A test case
title_full_unstemmed RAPD patterns in three Argentine Coprotus species: A test case
title_sort rapd patterns in three argentine coprotus species: a test case
publishDate 2008
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v103_n_p9_Ramos
http://hdl.handle.net/20.500.12110/paper_00934666_v103_n_p9_Ramos
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AT ranallidecintomariaesther rapdpatternsinthreeargentinecoprotusspeciesatestcase
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