Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
Transformation from morula to blastocyst is a defining event of preimplantation embryo development. During this transition, the embryo must establish a paracellular permeability barrier to enable expansion of the blastocyst cavity. Here, using live imaging of mouse embryos, we reveal an actin-zipper...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00928674_v173_n3_p776_Zenker http://hdl.handle.net/20.500.12110/paper_00928674_v173_n3_p776_Zenker |
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paper:paper_00928674_v173_n3_p776_Zenker2023-06-08T15:08:27Z Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation actin dynamics blastocyst cortical flow epithelia live imaging mammalian development microtubules morphogenesis preimplantation mouse embryo tight junctions actin myosin II actin cytoskeleton protein green fluorescent protein myosin II small interfering RNA actin capping actin filament animal cell Article bioaccumulation blastocyst cell junction developmental stage embryo live cell imaging microtubule mouse nonhuman priority journal protein localization protein structure animal blastocyst C57BL mouse cell communication chemistry embryo development female mammalian embryo metabolism morula three dimensional imaging tight junction Actins Animals Blastocyst Cell Communication Cytoskeletal Proteins Embryo, Mammalian Embryonic Development Female Green Fluorescent Proteins Imaging, Three-Dimensional Mice Mice, Inbred C57BL Microtubules Morula Myosin Type II RNA, Small Interfering Tight Junctions Transformation from morula to blastocyst is a defining event of preimplantation embryo development. During this transition, the embryo must establish a paracellular permeability barrier to enable expansion of the blastocyst cavity. Here, using live imaging of mouse embryos, we reveal an actin-zippering mechanism driving this embryo sealing. Preceding blastocyst stage, a cortical F-actin ring assembles at the apical pole of the embryo's outer cells. The ring structure forms when cortical actin flows encounter a network of polar microtubules that exclude F-actin. Unlike stereotypical actin rings, the actin rings of the mouse embryo are not contractile, but instead, they expand to the cell-cell junctions. Here, they couple to the junctions by recruiting and stabilizing adherens and tight junction components. Coupling of the actin rings triggers localized myosin II accumulation, and it initiates a tension-dependent zippering mechanism along the junctions that is required to seal the embryo for blastocyst formation. Expanding actin rings zipper the mouse embryo to seal it and allow formation of the blastocyst cavity. © 2018 Elsevier Inc. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00928674_v173_n3_p776_Zenker http://hdl.handle.net/20.500.12110/paper_00928674_v173_n3_p776_Zenker |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
actin dynamics blastocyst cortical flow epithelia live imaging mammalian development microtubules morphogenesis preimplantation mouse embryo tight junctions actin myosin II actin cytoskeleton protein green fluorescent protein myosin II small interfering RNA actin capping actin filament animal cell Article bioaccumulation blastocyst cell junction developmental stage embryo live cell imaging microtubule mouse nonhuman priority journal protein localization protein structure animal blastocyst C57BL mouse cell communication chemistry embryo development female mammalian embryo metabolism morula three dimensional imaging tight junction Actins Animals Blastocyst Cell Communication Cytoskeletal Proteins Embryo, Mammalian Embryonic Development Female Green Fluorescent Proteins Imaging, Three-Dimensional Mice Mice, Inbred C57BL Microtubules Morula Myosin Type II RNA, Small Interfering Tight Junctions |
| spellingShingle |
actin dynamics blastocyst cortical flow epithelia live imaging mammalian development microtubules morphogenesis preimplantation mouse embryo tight junctions actin myosin II actin cytoskeleton protein green fluorescent protein myosin II small interfering RNA actin capping actin filament animal cell Article bioaccumulation blastocyst cell junction developmental stage embryo live cell imaging microtubule mouse nonhuman priority journal protein localization protein structure animal blastocyst C57BL mouse cell communication chemistry embryo development female mammalian embryo metabolism morula three dimensional imaging tight junction Actins Animals Blastocyst Cell Communication Cytoskeletal Proteins Embryo, Mammalian Embryonic Development Female Green Fluorescent Proteins Imaging, Three-Dimensional Mice Mice, Inbred C57BL Microtubules Morula Myosin Type II RNA, Small Interfering Tight Junctions Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation |
| topic_facet |
actin dynamics blastocyst cortical flow epithelia live imaging mammalian development microtubules morphogenesis preimplantation mouse embryo tight junctions actin myosin II actin cytoskeleton protein green fluorescent protein myosin II small interfering RNA actin capping actin filament animal cell Article bioaccumulation blastocyst cell junction developmental stage embryo live cell imaging microtubule mouse nonhuman priority journal protein localization protein structure animal blastocyst C57BL mouse cell communication chemistry embryo development female mammalian embryo metabolism morula three dimensional imaging tight junction Actins Animals Blastocyst Cell Communication Cytoskeletal Proteins Embryo, Mammalian Embryonic Development Female Green Fluorescent Proteins Imaging, Three-Dimensional Mice Mice, Inbred C57BL Microtubules Morula Myosin Type II RNA, Small Interfering Tight Junctions |
| description |
Transformation from morula to blastocyst is a defining event of preimplantation embryo development. During this transition, the embryo must establish a paracellular permeability barrier to enable expansion of the blastocyst cavity. Here, using live imaging of mouse embryos, we reveal an actin-zippering mechanism driving this embryo sealing. Preceding blastocyst stage, a cortical F-actin ring assembles at the apical pole of the embryo's outer cells. The ring structure forms when cortical actin flows encounter a network of polar microtubules that exclude F-actin. Unlike stereotypical actin rings, the actin rings of the mouse embryo are not contractile, but instead, they expand to the cell-cell junctions. Here, they couple to the junctions by recruiting and stabilizing adherens and tight junction components. Coupling of the actin rings triggers localized myosin II accumulation, and it initiates a tension-dependent zippering mechanism along the junctions that is required to seal the embryo for blastocyst formation. Expanding actin rings zipper the mouse embryo to seal it and allow formation of the blastocyst cavity. © 2018 Elsevier Inc. |
| title |
Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation |
| title_short |
Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation |
| title_full |
Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation |
| title_fullStr |
Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation |
| title_full_unstemmed |
Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation |
| title_sort |
expanding actin rings zipper the mouse embryo for blastocyst formation |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00928674_v173_n3_p776_Zenker http://hdl.handle.net/20.500.12110/paper_00928674_v173_n3_p776_Zenker |
| _version_ |
1768542119642791936 |