Chromatin and alternative splicing

Alternative splicing affects more than 90% of human genes. Coupling between transcription and splicing has become crucial in the complex network underlying alternative splicing regulation. Because chromatin is the real template for nuclear transcription, changes in its structure, but also in the &qu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Allo, Mariano, Schor, Ignacio Esteban, Muñoz, Manuel Javier, de la Mata, Manuel, Kornblihtt, Alberto Rodolfo
Publicado:	2010
Materias:	double stranded RNA fibronectin messenger RNA mitogen activated protein kinase nerve cell adhesion molecule polypyrimidine tract binding protein RNA polymerase II small interfering RNA trichostatin A histone alternative RNA splicing article calcium signaling chromatin chromatin assembly and disassembly chromatin structure DNA fragmentation DNA methylation DNA replication DNA sequence exon gene mapping gene targeting genetic association genetic linkage heterochromatin histone acetylation human nerve cell differentiation nerve cell membrane steady potential nonhuman nucleosome posttranscriptional gene silencing priority journal promoter region RNA translation transcription regulation upregulation virus replication Article depolarization gene insertion gene silencing genome-wide association study histone modification medical literature molecular biology nerve cell stimulus Action Potentials Alternative Splicing Chromatin Chromatin Assembly and Disassembly DNA Replication Exons Histones Humans Models, Biological Neurons Nucleosomes
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00917451_v75_n_p103_Allo http://hdl.handle.net/20.500.12110/paper_00917451_v75_n_p103_Allo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00917451_v75_n_p103_Allo
record_format	dspace
spelling	paper:paper_00917451_v75_n_p103_Allo2023-06-08T15:08:09Z Chromatin and alternative splicing Allo, Mariano Schor, Ignacio Esteban Muñoz, Manuel Javier de la Mata, Manuel Kornblihtt, Alberto Rodolfo double stranded RNA fibronectin messenger RNA mitogen activated protein kinase nerve cell adhesion molecule polypyrimidine tract binding protein RNA polymerase II small interfering RNA trichostatin A histone alternative RNA splicing article calcium signaling chromatin chromatin assembly and disassembly chromatin structure DNA fragmentation DNA methylation DNA replication DNA sequence exon gene mapping gene targeting genetic association genetic linkage heterochromatin histone acetylation human nerve cell differentiation nerve cell membrane steady potential nonhuman nucleosome posttranscriptional gene silencing priority journal promoter region RNA translation transcription regulation upregulation virus replication Article depolarization gene insertion gene silencing genome-wide association study histone modification medical literature molecular biology nerve cell stimulus Action Potentials Alternative Splicing Chromatin Chromatin Assembly and Disassembly DNA Replication Exons Histones Humans Models, Biological Neurons Nucleosomes Alternative splicing affects more than 90% of human genes. Coupling between transcription and splicing has become crucial in the complex network underlying alternative splicing regulation. Because chromatin is the real template for nuclear transcription, changes in its structure, but also in the "reading" and "writing" of the histone code, could modulate splicing choices. Here, we discuss the evidence supporting these ideas, from the first proposal of chromatin affecting alternative splicing, performed 20 years ago, to the latest findings including genome-wide evidence that nucleosomes are preferentially positioned in exons. We focus on two recent reports from our laboratories that add new evidence to this field. The first report shows that a physiological stimulus such as neuron depolarization promotes intragenic histone acetylation (H3K9ac) and chromatin relaxation, causing the skipping of exon 18 of the neural cell adhesion molecule gene. In the second report, we show how specific histone modifications can be created at targeted gene regions as a way to affect alternative splicing: Using small interfering RNAs (siRNAs), we increased the levels of H3K9me2 and H3K27me3 in the proximity of alternative exon 33 of the human fibronectin gene, favoring its inclusion into mature messenger RNA (mRNA) through a mechanism that recalls RNAmediated transcriptional gene silencing. © 2010 Cold Spring Harbor Laboratory Press. Fil:Alló, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schor, I.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muñoz, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De La Mata, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00917451_v75_n_p103_Allo http://hdl.handle.net/20.500.12110/paper_00917451_v75_n_p103_Allo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	double stranded RNA fibronectin messenger RNA mitogen activated protein kinase nerve cell adhesion molecule polypyrimidine tract binding protein RNA polymerase II small interfering RNA trichostatin A histone alternative RNA splicing article calcium signaling chromatin chromatin assembly and disassembly chromatin structure DNA fragmentation DNA methylation DNA replication DNA sequence exon gene mapping gene targeting genetic association genetic linkage heterochromatin histone acetylation human nerve cell differentiation nerve cell membrane steady potential nonhuman nucleosome posttranscriptional gene silencing priority journal promoter region RNA translation transcription regulation upregulation virus replication Article depolarization gene insertion gene silencing genome-wide association study histone modification medical literature molecular biology nerve cell stimulus Action Potentials Alternative Splicing Chromatin Chromatin Assembly and Disassembly DNA Replication Exons Histones Humans Models, Biological Neurons Nucleosomes
spellingShingle	double stranded RNA fibronectin messenger RNA mitogen activated protein kinase nerve cell adhesion molecule polypyrimidine tract binding protein RNA polymerase II small interfering RNA trichostatin A histone alternative RNA splicing article calcium signaling chromatin chromatin assembly and disassembly chromatin structure DNA fragmentation DNA methylation DNA replication DNA sequence exon gene mapping gene targeting genetic association genetic linkage heterochromatin histone acetylation human nerve cell differentiation nerve cell membrane steady potential nonhuman nucleosome posttranscriptional gene silencing priority journal promoter region RNA translation transcription regulation upregulation virus replication Article depolarization gene insertion gene silencing genome-wide association study histone modification medical literature molecular biology nerve cell stimulus Action Potentials Alternative Splicing Chromatin Chromatin Assembly and Disassembly DNA Replication Exons Histones Humans Models, Biological Neurons Nucleosomes Allo, Mariano Schor, Ignacio Esteban Muñoz, Manuel Javier de la Mata, Manuel Kornblihtt, Alberto Rodolfo Chromatin and alternative splicing
topic_facet	double stranded RNA fibronectin messenger RNA mitogen activated protein kinase nerve cell adhesion molecule polypyrimidine tract binding protein RNA polymerase II small interfering RNA trichostatin A histone alternative RNA splicing article calcium signaling chromatin chromatin assembly and disassembly chromatin structure DNA fragmentation DNA methylation DNA replication DNA sequence exon gene mapping gene targeting genetic association genetic linkage heterochromatin histone acetylation human nerve cell differentiation nerve cell membrane steady potential nonhuman nucleosome posttranscriptional gene silencing priority journal promoter region RNA translation transcription regulation upregulation virus replication Article depolarization gene insertion gene silencing genome-wide association study histone modification medical literature molecular biology nerve cell stimulus Action Potentials Alternative Splicing Chromatin Chromatin Assembly and Disassembly DNA Replication Exons Histones Humans Models, Biological Neurons Nucleosomes
description	Alternative splicing affects more than 90% of human genes. Coupling between transcription and splicing has become crucial in the complex network underlying alternative splicing regulation. Because chromatin is the real template for nuclear transcription, changes in its structure, but also in the "reading" and "writing" of the histone code, could modulate splicing choices. Here, we discuss the evidence supporting these ideas, from the first proposal of chromatin affecting alternative splicing, performed 20 years ago, to the latest findings including genome-wide evidence that nucleosomes are preferentially positioned in exons. We focus on two recent reports from our laboratories that add new evidence to this field. The first report shows that a physiological stimulus such as neuron depolarization promotes intragenic histone acetylation (H3K9ac) and chromatin relaxation, causing the skipping of exon 18 of the neural cell adhesion molecule gene. In the second report, we show how specific histone modifications can be created at targeted gene regions as a way to affect alternative splicing: Using small interfering RNAs (siRNAs), we increased the levels of H3K9me2 and H3K27me3 in the proximity of alternative exon 33 of the human fibronectin gene, favoring its inclusion into mature messenger RNA (mRNA) through a mechanism that recalls RNAmediated transcriptional gene silencing. © 2010 Cold Spring Harbor Laboratory Press.
author	Allo, Mariano Schor, Ignacio Esteban Muñoz, Manuel Javier de la Mata, Manuel Kornblihtt, Alberto Rodolfo
author_facet	Allo, Mariano Schor, Ignacio Esteban Muñoz, Manuel Javier de la Mata, Manuel Kornblihtt, Alberto Rodolfo
author_sort	Allo, Mariano
title	Chromatin and alternative splicing
title_short	Chromatin and alternative splicing
title_full	Chromatin and alternative splicing
title_fullStr	Chromatin and alternative splicing
title_full_unstemmed	Chromatin and alternative splicing
title_sort	chromatin and alternative splicing
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00917451_v75_n_p103_Allo http://hdl.handle.net/20.500.12110/paper_00917451_v75_n_p103_Allo
work_keys_str_mv	AT allomariano chromatinandalternativesplicing AT schorignacioesteban chromatinandalternativesplicing AT munozmanueljavier chromatinandalternativesplicing AT delamatamanuel chromatinandalternativesplicing AT kornblihttalbertorodolfo chromatinandalternativesplicing
_version_	1768544632929517568

Chromatin and alternative splicing

Ejemplares similares