Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus

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Male crabs (Chasmagnathus granulatus) exhibited a defensive response (DR) to an electric shock (8 V, 50 Hz, 1 sec). The DR so elicited was used as a model for studying the antibociceptive effect of morphine. Injections of morphine-HCl (MP) (25, 50, 100 and 150 μg/g) were administered and the DR was...

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Detalles Bibliográficos
Autores principales: Lozada, Mariana, Romano, Arturo Gabriel
Publicado: 1988
Materias:
Antinociceptive effect
Arthropods
Defensive response
Morphine pharmacokinetics
morphine
naloxone
opiate receptor
animal experiment
antinociception
arthropod
crab
defensive behavior
electric shock
invertebrate
nonhuman
priority journal
psychological aspect
Animal
Behavior, Animal
Brachyura
Dose-Response Relationship, Drug
Electroshock
Morphine
Naloxone
Reference Values
Support, Non-U.S. Gov't
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00913057_v30_n3_p635_Lozada
http://hdl.handle.net/20.500.12110/paper_00913057_v30_n3_p635_Lozada
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00913057_v30_n3_p635_Lozada
record_format dspace
spelling paper:paper_00913057_v30_n3_p635_Lozada2023-06-08T15:08:02Z Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus Lozada, Mariana Romano, Arturo Gabriel Antinociceptive effect Arthropods Defensive response Morphine pharmacokinetics morphine naloxone opiate receptor animal experiment antinociception arthropod crab defensive behavior electric shock invertebrate nonhuman priority journal psychological aspect Animal Behavior, Animal Brachyura Dose-Response Relationship, Drug Electroshock Morphine Naloxone Reference Values Support, Non-U.S. Gov't Male crabs (Chasmagnathus granulatus) exhibited a defensive response (DR) to an electric shock (8 V, 50 Hz, 1 sec). The DR so elicited was used as a model for studying the antibociceptive effect of morphine. Injections of morphine-HCl (MP) (25, 50, 100 and 150 μg/g) were administered and the DR was examined at 2, 7.5, 15, 30, 45 and 75 min post-injection. (a) MP produced a dose-dependent reduction of the crab's sensitivity to the nociceptive stimulus. (b) A 100 μg/g dose of MP caused a 50% response inhibition with an injection-shock interval of 30 minutes, but no inhibition occurred when the same dose was administered with 1.6 μg/g of naloxone-HCl, suggesting that MP acts through an opiate receptor. (c) The ED50 at 2 min post-injection was roughly 33 μg/g and the threshold dose was estimated to be 6.8 μg/g. These doses are lower than ED50 values reported for other arthropods (90 to 930 μg/g) and approach those of vertebrates. (d) The peak MP effect was reached quickly, within 2 min post-injection. The duration of the MP effect was calculated to be 45.0-75.0 min depending on the dose, and an indirect estimate of half-life elimination was 15.7 min. These values are remarkably lower than those reported for vertebrates. The shorter duration of the MP peak effect is attributable to a greater permeability of the arthropod blood-brain barrier as compared to that of verebrates. © 1988. Fil:Lozada, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romano, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00913057_v30_n3_p635_Lozada http://hdl.handle.net/20.500.12110/paper_00913057_v30_n3_p635_Lozada
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antinociceptive effect
Arthropods
Defensive response
Morphine pharmacokinetics
morphine
naloxone
opiate receptor
animal experiment
antinociception
arthropod
crab
defensive behavior
electric shock
invertebrate
nonhuman
priority journal
psychological aspect
Animal
Behavior, Animal
Brachyura
Dose-Response Relationship, Drug
Electroshock
Morphine
Naloxone
Reference Values
Support, Non-U.S. Gov't
spellingShingle Antinociceptive effect
Arthropods
Defensive response
Morphine pharmacokinetics
morphine
naloxone
opiate receptor
animal experiment
antinociception
arthropod
crab
defensive behavior
electric shock
invertebrate
nonhuman
priority journal
psychological aspect
Animal
Behavior, Animal
Brachyura
Dose-Response Relationship, Drug
Electroshock
Morphine
Naloxone
Reference Values
Support, Non-U.S. Gov't
Lozada, Mariana
Romano, Arturo Gabriel
Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus
topic_facet Antinociceptive effect
Arthropods
Defensive response
Morphine pharmacokinetics
morphine
naloxone
opiate receptor
animal experiment
antinociception
arthropod
crab
defensive behavior
electric shock
invertebrate
nonhuman
priority journal
psychological aspect
Animal
Behavior, Animal
Brachyura
Dose-Response Relationship, Drug
Electroshock
Morphine
Naloxone
Reference Values
Support, Non-U.S. Gov't
description Male crabs (Chasmagnathus granulatus) exhibited a defensive response (DR) to an electric shock (8 V, 50 Hz, 1 sec). The DR so elicited was used as a model for studying the antibociceptive effect of morphine. Injections of morphine-HCl (MP) (25, 50, 100 and 150 μg/g) were administered and the DR was examined at 2, 7.5, 15, 30, 45 and 75 min post-injection. (a) MP produced a dose-dependent reduction of the crab's sensitivity to the nociceptive stimulus. (b) A 100 μg/g dose of MP caused a 50% response inhibition with an injection-shock interval of 30 minutes, but no inhibition occurred when the same dose was administered with 1.6 μg/g of naloxone-HCl, suggesting that MP acts through an opiate receptor. (c) The ED50 at 2 min post-injection was roughly 33 μg/g and the threshold dose was estimated to be 6.8 μg/g. These doses are lower than ED50 values reported for other arthropods (90 to 930 μg/g) and approach those of vertebrates. (d) The peak MP effect was reached quickly, within 2 min post-injection. The duration of the MP effect was calculated to be 45.0-75.0 min depending on the dose, and an indirect estimate of half-life elimination was 15.7 min. These values are remarkably lower than those reported for vertebrates. The shorter duration of the MP peak effect is attributable to a greater permeability of the arthropod blood-brain barrier as compared to that of verebrates. © 1988.
author Lozada, Mariana
Romano, Arturo Gabriel
author_facet Lozada, Mariana
Romano, Arturo Gabriel
author_sort Lozada, Mariana
title Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus
title_short Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus
title_full Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus
title_fullStr Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus
title_full_unstemmed Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus
title_sort effect of morphine and naloxone on a defensive response of the crab chasmagnathus granulatus
publishDate 1988
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00913057_v30_n3_p635_Lozada
http://hdl.handle.net/20.500.12110/paper_00913057_v30_n3_p635_Lozada
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AT romanoarturogabriel effectofmorphineandnaloxoneonadefensiveresponseofthecrabchasmagnathusgranulatus
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