Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats
Formation of {3H}-PGF2α and {3H}-13,14,dihydro-15-keto-PGF2α from {3H}-PGE2 by the supernatant of uterine homogenates from estrous and ovariectomized rats, was studied, using the reaction system PGE2 + NADPH + {3H}-PGE2 + supernatant. Enzymatic conversion was lower in uterine supernatants from spaye...
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1985
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00906980_v29_n5_p773_Franchi http://hdl.handle.net/20.500.12110/paper_00906980_v29_n5_p773_Franchi |
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paper:paper_00906980_v29_n5_p773_Franchi2023-06-08T15:07:56Z Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats estradiol progesterone prostaglandin e2 prostaglandin f2 alpha radioisotope animal cell drug efficacy endocrine system female genital system myometrium nonhuman ovariectomy priority journal prostaglandin e2 h 3 rat Animal Castration Dinoprost Dinoprostone Estradiol Female NADP Progesterone Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Support, Non-U.S. Gov't Uterus Formation of {3H}-PGF2α and {3H}-13,14,dihydro-15-keto-PGF2α from {3H}-PGE2 by the supernatant of uterine homogenates from estrous and ovariectomized rats, was studied, using the reaction system PGE2 + NADPH + {3H}-PGE2 + supernatant. Enzymatic conversion was lower in uterine supernatants from spayed rats than in uterine homogenates of rats at natural estrus. Spayed animals were injected with progesterone (P) or with estradiol-17-β (E0) at a dose of 1.0 or 50.0 ug. Conversion of {3H}-PGF2α to {3H}-PGE2 or to {3H}-13,14,dihydro-15-keto-PGF2α did not differ in control ovariectomized or ovariectomized rats receiving P or 1.0 ug E0. However, 50.0 ug E0 induced a significant oversion after 30 (P < 0.01) and 60 (P < 0.001) min of incubation. It is concluded that E0, at the 50.0 ug dose, but not the 1.0 ug dose of E0, nor progesterone, stimulated conversion of {3H}-PGE2 into {3H}-PGF2α or {3H}-13, 14,dihydro-15-keto-PGF2α, presumably through the activity of the enzyme PGE2-9-keto-reductase. © 1985. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00906980_v29_n5_p773_Franchi http://hdl.handle.net/20.500.12110/paper_00906980_v29_n5_p773_Franchi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
estradiol progesterone prostaglandin e2 prostaglandin f2 alpha radioisotope animal cell drug efficacy endocrine system female genital system myometrium nonhuman ovariectomy priority journal prostaglandin e2 h 3 rat Animal Castration Dinoprost Dinoprostone Estradiol Female NADP Progesterone Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Support, Non-U.S. Gov't Uterus |
| spellingShingle |
estradiol progesterone prostaglandin e2 prostaglandin f2 alpha radioisotope animal cell drug efficacy endocrine system female genital system myometrium nonhuman ovariectomy priority journal prostaglandin e2 h 3 rat Animal Castration Dinoprost Dinoprostone Estradiol Female NADP Progesterone Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Support, Non-U.S. Gov't Uterus Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats |
| topic_facet |
estradiol progesterone prostaglandin e2 prostaglandin f2 alpha radioisotope animal cell drug efficacy endocrine system female genital system myometrium nonhuman ovariectomy priority journal prostaglandin e2 h 3 rat Animal Castration Dinoprost Dinoprostone Estradiol Female NADP Progesterone Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Support, Non-U.S. Gov't Uterus |
| description |
Formation of {3H}-PGF2α and {3H}-13,14,dihydro-15-keto-PGF2α from {3H}-PGE2 by the supernatant of uterine homogenates from estrous and ovariectomized rats, was studied, using the reaction system PGE2 + NADPH + {3H}-PGE2 + supernatant. Enzymatic conversion was lower in uterine supernatants from spayed rats than in uterine homogenates of rats at natural estrus. Spayed animals were injected with progesterone (P) or with estradiol-17-β (E0) at a dose of 1.0 or 50.0 ug. Conversion of {3H}-PGF2α to {3H}-PGE2 or to {3H}-13,14,dihydro-15-keto-PGF2α did not differ in control ovariectomized or ovariectomized rats receiving P or 1.0 ug E0. However, 50.0 ug E0 induced a significant oversion after 30 (P < 0.01) and 60 (P < 0.001) min of incubation. It is concluded that E0, at the 50.0 ug dose, but not the 1.0 ug dose of E0, nor progesterone, stimulated conversion of {3H}-PGE2 into {3H}-PGF2α or {3H}-13, 14,dihydro-15-keto-PGF2α, presumably through the activity of the enzyme PGE2-9-keto-reductase. © 1985. |
| title |
Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats |
| title_short |
Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats |
| title_full |
Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats |
| title_fullStr |
Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats |
| title_full_unstemmed |
Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats |
| title_sort |
estradiol -17-β enhances the formation of {3h}-pgf2α from {3h}-pge2 in the uterus isolated from ovariectomized rats |
| publishDate |
1985 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00906980_v29_n5_p773_Franchi http://hdl.handle.net/20.500.12110/paper_00906980_v29_n5_p773_Franchi |
| _version_ |
1768544724024557568 |