Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins

The trypanocidal action of green tea catechins against two different developmental stages of Trypanosoma cruzi is reported for the first time. This activity was assayed with the nonproliferative bloodstream trypomastigote and with the intracellular replicative amastigote parasite forms. An ethyl ace...

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Autores principales:	Paveto, María Cristina, Guida, María Clara, Flawiá, Mirtha María, Torres, Héctor Norberto
Publicado:	2004
Materias:	acetic acid ethyl ester arginine kinase catechin catechin gallate epicatechin epicatechin gallate epigallocatechin epigallocatechin gallate flavanol derivative gallocatechin gallocatechin gallate green tea extract organic solvent polyphenol derivative unclassified drug amastigote animal cell animal experiment animal model antiprotozoal activity aqueous solution article bactericidal activity blood blood flow column chromatography concentration response controlled study developmental stage diode drug determination drug effect drug efficacy drug identification drug mechanism drug purification drug screening energy metabolism enzyme inactivation enzyme inhibition fractionation gas chromatography high performance liquid chromatography lysis male mass spectrometry minimum inhibitory concentration mouse nonhuman plant leaf priority journal protein function protozoal infection tea Trypanosoma cruzi trypomastigote Vero cell Animals Arginine Kinase Camellia Catechin Cercopithecus aethiops Chagas Disease Chromatography, Gas Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Indicators and Reagents Male Mice Mice, Inbred BALB C Plant Extracts Trypanocidal Agents Vero Cells
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00664804_v48_n1_p69_Paveto http://hdl.handle.net/20.500.12110/paper_00664804_v48_n1_p69_Paveto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00664804_v48_n1_p69_Paveto2023-06-08T15:06:10Z Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins Paveto, María Cristina Guida, María Clara Flawiá, Mirtha María Torres, Héctor Norberto acetic acid ethyl ester arginine kinase catechin catechin gallate epicatechin epicatechin gallate epigallocatechin epigallocatechin gallate flavanol derivative gallocatechin gallocatechin gallate green tea extract organic solvent polyphenol derivative unclassified drug amastigote animal cell animal experiment animal model antiprotozoal activity aqueous solution article bactericidal activity blood blood flow column chromatography concentration response controlled study developmental stage diode drug determination drug effect drug efficacy drug identification drug mechanism drug purification drug screening energy metabolism enzyme inactivation enzyme inhibition fractionation gas chromatography high performance liquid chromatography lysis male mass spectrometry minimum inhibitory concentration mouse nonhuman plant leaf priority journal protein function protozoal infection tea Trypanosoma cruzi trypomastigote Vero cell Animals Arginine Kinase Camellia Catechin Cercopithecus aethiops Chagas Disease Chromatography, Gas Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Indicators and Reagents Male Mice Mice, Inbred BALB C Plant Extracts Trypanocidal Agents Trypanosoma cruzi Vero Cells The trypanocidal action of green tea catechins against two different developmental stages of Trypanosoma cruzi is reported for the first time. This activity was assayed with the nonproliferative bloodstream trypomastigote and with the intracellular replicative amastigote parasite forms. An ethyl acetate fraction from Camellia sinensis green tea leaves, which contains most of the polyphenolic compounds and the maximal trypanocidal activity, was obtained by fractionation of the aqueous extract with organic solvents. The active compounds present in this extract were further purified by LH-20 column chromatography and were identified by high-performance liquid chromatography analysis with a photo diode array detector and gas chromatography coupled to mass spectroscopy. The following flavan-3-ols derivatives, known as catechins, were identified: catechin, epicatechin, gallocatechin, epigallocatechin, catechin gallate, epicatechin gallate, gallocatechin gallate, and epigallocatechin gallate. The purified compounds lysed more than 50% of the parasites present in the blood of infected BALB/c mice at concentrations as low as 0.12 to 85 pM. The most active compounds were gallocatechin gallate and epigallocatechin gallate, with minimal bactericidal concentrations that inhibited 50% of isolates tested of 0.12 and 0.53 pM, respectively. The number of amastigotes in infected Vero cells decreased by 50% in the presence of each of these compounds at 100 nM. The effects of the catechins on the recombinant T. cruzi arginine kinase, a key enzyme in the energy metabolism of the parasite, were assayed. The activity of this enzyme was inhibited by about 50% by nanomolar concentrations of catechin gallate or gallocatechin gallate, whereas the other members of the group were less effective. On the basis of these results, we suggest that these compounds could be used to sterilize blood and, eventually, as therapeutic agents for Chagas' disease. Fil:Paveto, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Güida, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00664804_v48_n1_p69_Paveto http://hdl.handle.net/20.500.12110/paper_00664804_v48_n1_p69_Paveto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acetic acid ethyl ester arginine kinase catechin catechin gallate epicatechin epicatechin gallate epigallocatechin epigallocatechin gallate flavanol derivative gallocatechin gallocatechin gallate green tea extract organic solvent polyphenol derivative unclassified drug amastigote animal cell animal experiment animal model antiprotozoal activity aqueous solution article bactericidal activity blood blood flow column chromatography concentration response controlled study developmental stage diode drug determination drug effect drug efficacy drug identification drug mechanism drug purification drug screening energy metabolism enzyme inactivation enzyme inhibition fractionation gas chromatography high performance liquid chromatography lysis male mass spectrometry minimum inhibitory concentration mouse nonhuman plant leaf priority journal protein function protozoal infection tea Trypanosoma cruzi trypomastigote Vero cell Animals Arginine Kinase Camellia Catechin Cercopithecus aethiops Chagas Disease Chromatography, Gas Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Indicators and Reagents Male Mice Mice, Inbred BALB C Plant Extracts Trypanocidal Agents Trypanosoma cruzi Vero Cells
spellingShingle	acetic acid ethyl ester arginine kinase catechin catechin gallate epicatechin epicatechin gallate epigallocatechin epigallocatechin gallate flavanol derivative gallocatechin gallocatechin gallate green tea extract organic solvent polyphenol derivative unclassified drug amastigote animal cell animal experiment animal model antiprotozoal activity aqueous solution article bactericidal activity blood blood flow column chromatography concentration response controlled study developmental stage diode drug determination drug effect drug efficacy drug identification drug mechanism drug purification drug screening energy metabolism enzyme inactivation enzyme inhibition fractionation gas chromatography high performance liquid chromatography lysis male mass spectrometry minimum inhibitory concentration mouse nonhuman plant leaf priority journal protein function protozoal infection tea Trypanosoma cruzi trypomastigote Vero cell Animals Arginine Kinase Camellia Catechin Cercopithecus aethiops Chagas Disease Chromatography, Gas Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Indicators and Reagents Male Mice Mice, Inbred BALB C Plant Extracts Trypanocidal Agents Trypanosoma cruzi Vero Cells Paveto, María Cristina Guida, María Clara Flawiá, Mirtha María Torres, Héctor Norberto Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins
topic_facet	acetic acid ethyl ester arginine kinase catechin catechin gallate epicatechin epicatechin gallate epigallocatechin epigallocatechin gallate flavanol derivative gallocatechin gallocatechin gallate green tea extract organic solvent polyphenol derivative unclassified drug amastigote animal cell animal experiment animal model antiprotozoal activity aqueous solution article bactericidal activity blood blood flow column chromatography concentration response controlled study developmental stage diode drug determination drug effect drug efficacy drug identification drug mechanism drug purification drug screening energy metabolism enzyme inactivation enzyme inhibition fractionation gas chromatography high performance liquid chromatography lysis male mass spectrometry minimum inhibitory concentration mouse nonhuman plant leaf priority journal protein function protozoal infection tea Trypanosoma cruzi trypomastigote Vero cell Animals Arginine Kinase Camellia Catechin Cercopithecus aethiops Chagas Disease Chromatography, Gas Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Indicators and Reagents Male Mice Mice, Inbred BALB C Plant Extracts Trypanocidal Agents Trypanosoma cruzi Vero Cells
description	The trypanocidal action of green tea catechins against two different developmental stages of Trypanosoma cruzi is reported for the first time. This activity was assayed with the nonproliferative bloodstream trypomastigote and with the intracellular replicative amastigote parasite forms. An ethyl acetate fraction from Camellia sinensis green tea leaves, which contains most of the polyphenolic compounds and the maximal trypanocidal activity, was obtained by fractionation of the aqueous extract with organic solvents. The active compounds present in this extract were further purified by LH-20 column chromatography and were identified by high-performance liquid chromatography analysis with a photo diode array detector and gas chromatography coupled to mass spectroscopy. The following flavan-3-ols derivatives, known as catechins, were identified: catechin, epicatechin, gallocatechin, epigallocatechin, catechin gallate, epicatechin gallate, gallocatechin gallate, and epigallocatechin gallate. The purified compounds lysed more than 50% of the parasites present in the blood of infected BALB/c mice at concentrations as low as 0.12 to 85 pM. The most active compounds were gallocatechin gallate and epigallocatechin gallate, with minimal bactericidal concentrations that inhibited 50% of isolates tested of 0.12 and 0.53 pM, respectively. The number of amastigotes in infected Vero cells decreased by 50% in the presence of each of these compounds at 100 nM. The effects of the catechins on the recombinant T. cruzi arginine kinase, a key enzyme in the energy metabolism of the parasite, were assayed. The activity of this enzyme was inhibited by about 50% by nanomolar concentrations of catechin gallate or gallocatechin gallate, whereas the other members of the group were less effective. On the basis of these results, we suggest that these compounds could be used to sterilize blood and, eventually, as therapeutic agents for Chagas' disease.
author	Paveto, María Cristina Guida, María Clara Flawiá, Mirtha María Torres, Héctor Norberto
author_facet	Paveto, María Cristina Guida, María Clara Flawiá, Mirtha María Torres, Héctor Norberto
author_sort	Paveto, María Cristina
title	Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins
title_short	Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins
title_full	Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins
title_fullStr	Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins
title_full_unstemmed	Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins
title_sort	anti-trypanosoma cruzi activity of green tea (camellia sinensis) catechins
publishDate	2004
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00664804_v48_n1_p69_Paveto http://hdl.handle.net/20.500.12110/paper_00664804_v48_n1_p69_Paveto
work_keys_str_mv	AT pavetomariacristina antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins AT guidamariaclara antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins AT flawiamirthamaria antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins AT torreshectornorberto antitrypanosomacruziactivityofgreenteacamelliasinensiscatechins
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Anti-Trypanosoma cruzi Activity of Green Tea (Camellia sinensis) Catechins

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