A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly idu...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v113_n3-4_p243_Calabrese http://hdl.handle.net/20.500.12110/paper_00493848_v113_n3-4_p243_Calabrese |
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paper:paper_00493848_v113_n3-4_p243_Calabrese2023-06-08T15:05:47Z A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein Recondo, Eduardo Francisco antithrombin AT C1 Complement system Con A Concanavalin A Dermatan sulfate DS free Dermatan sulfate free DS GAG glycosaminoglycan Heparin cofactor II HS Molecular interactions the first protein complex of the complement system calcium ion dermatan sulfate fibrinolytic agent heparin cofactor II plasma protein protein complex c1 unclassified drug animal experiment animal model animal tissue anticoagulation article complement system dissolution drug activity drug structure human ionic strength male nonhuman normal human precipitation priority journal rat thrombosis Acetylgalactosamine Animals Anticoagulants Calcium Complement C1 Dermatan Sulfate Fibrinolytic Agents Hexosamines Iduronic Acid Male Osmolar Concentration Precipitation Rats Rats, Wistar Structure-Activity Relationship Sulfates Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly iduronic, joined 1→3 to the hexosamine. We are studying the relationships between structure and activities of dermatan sulfate, in particular those associated with the thrombin inhibition mediated by heparin cofactor II (HCII). As we have demonstrated with heparin, a small fraction of dermatan sulfate was isolated by precipitation with the first component of the complement system, under very specific conditions of low ionic strength, and the presence of calcium ions. The sulfate content and the anticoagulant activity of the dermatan sulfate fraction isolated in the precipitate were three and four times greater respectively than the starting material. Our in vivo studies showed that this fraction has threefold higher thrombolytic activity than the DS. All these results suggest that this fraction could be used as a therapeutic agent for thrombi dissolution. © 2004 Elsevier Ltd. All rights reserved. Fil:Recondo, E.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v113_n3-4_p243_Calabrese http://hdl.handle.net/20.500.12110/paper_00493848_v113_n3-4_p243_Calabrese |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
antithrombin AT C1 Complement system Con A Concanavalin A Dermatan sulfate DS free Dermatan sulfate free DS GAG glycosaminoglycan Heparin cofactor II HS Molecular interactions the first protein complex of the complement system calcium ion dermatan sulfate fibrinolytic agent heparin cofactor II plasma protein protein complex c1 unclassified drug animal experiment animal model animal tissue anticoagulation article complement system dissolution drug activity drug structure human ionic strength male nonhuman normal human precipitation priority journal rat thrombosis Acetylgalactosamine Animals Anticoagulants Calcium Complement C1 Dermatan Sulfate Fibrinolytic Agents Hexosamines Iduronic Acid Male Osmolar Concentration Precipitation Rats Rats, Wistar Structure-Activity Relationship Sulfates |
| spellingShingle |
antithrombin AT C1 Complement system Con A Concanavalin A Dermatan sulfate DS free Dermatan sulfate free DS GAG glycosaminoglycan Heparin cofactor II HS Molecular interactions the first protein complex of the complement system calcium ion dermatan sulfate fibrinolytic agent heparin cofactor II plasma protein protein complex c1 unclassified drug animal experiment animal model animal tissue anticoagulation article complement system dissolution drug activity drug structure human ionic strength male nonhuman normal human precipitation priority journal rat thrombosis Acetylgalactosamine Animals Anticoagulants Calcium Complement C1 Dermatan Sulfate Fibrinolytic Agents Hexosamines Iduronic Acid Male Osmolar Concentration Precipitation Rats Rats, Wistar Structure-Activity Relationship Sulfates Recondo, Eduardo Francisco A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein |
| topic_facet |
antithrombin AT C1 Complement system Con A Concanavalin A Dermatan sulfate DS free Dermatan sulfate free DS GAG glycosaminoglycan Heparin cofactor II HS Molecular interactions the first protein complex of the complement system calcium ion dermatan sulfate fibrinolytic agent heparin cofactor II plasma protein protein complex c1 unclassified drug animal experiment animal model animal tissue anticoagulation article complement system dissolution drug activity drug structure human ionic strength male nonhuman normal human precipitation priority journal rat thrombosis Acetylgalactosamine Animals Anticoagulants Calcium Complement C1 Dermatan Sulfate Fibrinolytic Agents Hexosamines Iduronic Acid Male Osmolar Concentration Precipitation Rats Rats, Wistar Structure-Activity Relationship Sulfates |
| description |
Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly iduronic, joined 1→3 to the hexosamine. We are studying the relationships between structure and activities of dermatan sulfate, in particular those associated with the thrombin inhibition mediated by heparin cofactor II (HCII). As we have demonstrated with heparin, a small fraction of dermatan sulfate was isolated by precipitation with the first component of the complement system, under very specific conditions of low ionic strength, and the presence of calcium ions. The sulfate content and the anticoagulant activity of the dermatan sulfate fraction isolated in the precipitate were three and four times greater respectively than the starting material. Our in vivo studies showed that this fraction has threefold higher thrombolytic activity than the DS. All these results suggest that this fraction could be used as a therapeutic agent for thrombi dissolution. © 2004 Elsevier Ltd. All rights reserved. |
| author |
Recondo, Eduardo Francisco |
| author_facet |
Recondo, Eduardo Francisco |
| author_sort |
Recondo, Eduardo Francisco |
| title |
A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein |
| title_short |
A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein |
| title_full |
A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein |
| title_fullStr |
A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein |
| title_full_unstemmed |
A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein |
| title_sort |
small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v113_n3-4_p243_Calabrese http://hdl.handle.net/20.500.12110/paper_00493848_v113_n3-4_p243_Calabrese |
| work_keys_str_mv |
AT recondoeduardofrancisco asmallfractionofdermatansulfatewithsignificantlyincreasedanticoagulantactivitywasselectedbyinteractionwiththefirstcomplementprotein AT recondoeduardofrancisco smallfractionofdermatansulfatewithsignificantlyincreasedanticoagulantactivitywasselectedbyinteractionwiththefirstcomplementprotein |
| _version_ |
1768543506121359360 |