Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in Planorbarius corneus
In this study, the cholinesterase (ChE) and carboxylesterase (CES) activities present in whole organism homogenates from Planorbarius corneus and their in vitro sensitivity to organophosphorous (OP) pesticides were studied. Firstly, a characterization of ChE and CES activities using different substr...
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| Publicado: |
2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00456535_v88_n4_p450_Cacciatore http://hdl.handle.net/20.500.12110/paper_00456535_v88_n4_p450_Cacciatore |
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paper:paper_00456535_v88_n4_p450_Cacciatore |
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dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Carboxylesterase Cholinesterase Invertebrate Mixtures Pesticides Active oxygen Azinphosmethyl Binary combinations Carboxylesterases Chlorpyrifos-oxon Cholinesterase Cholinesterase inhibition Different substrates Dose-response curves High concentration Hydrolyzing activity In-vitro In-vivo Invertebrate Low concentrations Organophosphorous P-nitrophenyl acetate Selective inhibitors Specific activity Synergistic effect Hydrolysis Ionization of gases Mixtures Naphthalene Pesticides Substrates Volatile fatty acids Binary mixtures 1 naphthyl acetate 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide 2 naphthyl acetate 4 nitrophenyl acetate acetylthiocholine butyric acid 4 nitrophenyl ester cholinesterase insecticide pesticide unclassified drug acetate antagonism concentration (composition) dose-response relationship enzyme activity inhibition inhibitor insecticide oxygen snail animal experiment animal tissue article concentration (parameters) dose response enzyme inhibition enzyme substrate homogenate hydrolysis IC 50 in vitro study nonhuman Planorabarius corneus snail Acanthaceae Azinphosmethyl Carboxylesterase Chlorpyrifos Cholinesterase Inhibitors Cholinesterases Drug Synergism Oxygen Pesticides Gastropoda Invertebrata Planorbarius corneus |
| spellingShingle |
Carboxylesterase Cholinesterase Invertebrate Mixtures Pesticides Active oxygen Azinphosmethyl Binary combinations Carboxylesterases Chlorpyrifos-oxon Cholinesterase Cholinesterase inhibition Different substrates Dose-response curves High concentration Hydrolyzing activity In-vitro In-vivo Invertebrate Low concentrations Organophosphorous P-nitrophenyl acetate Selective inhibitors Specific activity Synergistic effect Hydrolysis Ionization of gases Mixtures Naphthalene Pesticides Substrates Volatile fatty acids Binary mixtures 1 naphthyl acetate 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide 2 naphthyl acetate 4 nitrophenyl acetate acetylthiocholine butyric acid 4 nitrophenyl ester cholinesterase insecticide pesticide unclassified drug acetate antagonism concentration (composition) dose-response relationship enzyme activity inhibition inhibitor insecticide oxygen snail animal experiment animal tissue article concentration (parameters) dose response enzyme inhibition enzyme substrate homogenate hydrolysis IC 50 in vitro study nonhuman Planorabarius corneus snail Acanthaceae Azinphosmethyl Carboxylesterase Chlorpyrifos Cholinesterase Inhibitors Cholinesterases Drug Synergism Oxygen Pesticides Gastropoda Invertebrata Planorbarius corneus Kristoff, Gisela Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in Planorbarius corneus |
| topic_facet |
Carboxylesterase Cholinesterase Invertebrate Mixtures Pesticides Active oxygen Azinphosmethyl Binary combinations Carboxylesterases Chlorpyrifos-oxon Cholinesterase Cholinesterase inhibition Different substrates Dose-response curves High concentration Hydrolyzing activity In-vitro In-vivo Invertebrate Low concentrations Organophosphorous P-nitrophenyl acetate Selective inhibitors Specific activity Synergistic effect Hydrolysis Ionization of gases Mixtures Naphthalene Pesticides Substrates Volatile fatty acids Binary mixtures 1 naphthyl acetate 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide 2 naphthyl acetate 4 nitrophenyl acetate acetylthiocholine butyric acid 4 nitrophenyl ester cholinesterase insecticide pesticide unclassified drug acetate antagonism concentration (composition) dose-response relationship enzyme activity inhibition inhibitor insecticide oxygen snail animal experiment animal tissue article concentration (parameters) dose response enzyme inhibition enzyme substrate homogenate hydrolysis IC 50 in vitro study nonhuman Planorabarius corneus snail Acanthaceae Azinphosmethyl Carboxylesterase Chlorpyrifos Cholinesterase Inhibitors Cholinesterases Drug Synergism Oxygen Pesticides Gastropoda Invertebrata Planorbarius corneus |
| description |
In this study, the cholinesterase (ChE) and carboxylesterase (CES) activities present in whole organism homogenates from Planorbarius corneus and their in vitro sensitivity to organophosphorous (OP) pesticides were studied. Firstly, a characterization of ChE and CES activities using different substrates and selective inhibitors was performed. Secondly, the effects of azinphos-methyl oxon (AZM-oxon) and chlorpyrifos oxon (CPF-oxon), the active oxygen analogs of the OP insecticides AZM and CPF, on ChE and CES activities were evaluated. Finally, it was analyzed whether binary mixtures of the pesticide oxons cause additive, antagonistic or synergistic ChE inhibition in P. corneus homogenates. The results showed that the extracts of P. corneus preferentially hydrolyzed acetylthiocholine (AcSCh) over propionylthiocholine (PrSCh) and butyrylthiocholine (BuSCh). Besides, AcSCh hydrolyzing activity was inhibited by low concentrations of BW284c51, a selective inhibitor of AChE activity, and also by high concentrations of substrate. These facts suggest the presence of a typical AChE activity in this species. However, the different dose-response curves observed with BW284c51 when using PrSCh or BuSCh instead of AcSCh suggest the presence of at least another ChE activity. This would probably correspond to an atypical BuChE. Regarding CES activity, the highest specific activity was obtained when using 2-naphthyl acetate (2-NA), followed by 1-naphthyl acetate (1-NA); p-nitrophenyl acetate (p-NPA), and p-nitrophenyl butyrate (p-NPB). The comparison of the IC50 values revealed that, regardless of the substrate used, CES activity was approximately one order of magnitude more sensitive to AZM-oxon than ChE activity. Although ChE activity was very sensitive to CPF-oxon, CES activity measured with 1-NA, 2-NA, and p-NPA was poorly inhibited by this pesticide. In contrast, CES activity measured with p-NPB was equally sensitive to CPF-oxon than ChE activity. Several specific binary combinations of AZM-oxon and CPF-oxon caused a synergistic effect on the ChE inhibition in P. corneus homogenates. The degree of synergism tended to increase as the ratio of AZM-oxon to CPF-oxon decreased. These results suggest that synergism is likely to occur in P. corneus snails exposed in vivo to binary mixtures of the OPs AZM and CPF. © 2012 Elsevier Ltd. |
| author |
Kristoff, Gisela Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina |
| author_facet |
Kristoff, Gisela Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina |
| author_sort |
Kristoff, Gisela |
| title |
Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in Planorbarius corneus |
| title_short |
Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in Planorbarius corneus |
| title_full |
Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in Planorbarius corneus |
| title_fullStr |
Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in Planorbarius corneus |
| title_full_unstemmed |
Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in Planorbarius corneus |
| title_sort |
binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in planorbarius corneus |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00456535_v88_n4_p450_Cacciatore http://hdl.handle.net/20.500.12110/paper_00456535_v88_n4_p450_Cacciatore |
| work_keys_str_mv |
AT kristoffgisela binarymixturesofazinphosmethyloxonandchlorpyrifosoxonproduceinvitrosynergisticcholinesteraseinhibitioninplanorbariuscorneus AT verrengiaguerreronoemirosario binarymixturesofazinphosmethyloxonandchlorpyrifosoxonproduceinvitrosynergisticcholinesteraseinhibitioninplanorbariuscorneus AT cochonadrianacristina binarymixturesofazinphosmethyloxonandchlorpyrifosoxonproduceinvitrosynergisticcholinesteraseinhibitioninplanorbariuscorneus |
| _version_ |
1768544543888637952 |
| spelling |
paper:paper_00456535_v88_n4_p450_Cacciatore2023-06-08T15:05:20Z Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in Planorbarius corneus Kristoff, Gisela Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina Carboxylesterase Cholinesterase Invertebrate Mixtures Pesticides Active oxygen Azinphosmethyl Binary combinations Carboxylesterases Chlorpyrifos-oxon Cholinesterase Cholinesterase inhibition Different substrates Dose-response curves High concentration Hydrolyzing activity In-vitro In-vivo Invertebrate Low concentrations Organophosphorous P-nitrophenyl acetate Selective inhibitors Specific activity Synergistic effect Hydrolysis Ionization of gases Mixtures Naphthalene Pesticides Substrates Volatile fatty acids Binary mixtures 1 naphthyl acetate 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide 2 naphthyl acetate 4 nitrophenyl acetate acetylthiocholine butyric acid 4 nitrophenyl ester cholinesterase insecticide pesticide unclassified drug acetate antagonism concentration (composition) dose-response relationship enzyme activity inhibition inhibitor insecticide oxygen snail animal experiment animal tissue article concentration (parameters) dose response enzyme inhibition enzyme substrate homogenate hydrolysis IC 50 in vitro study nonhuman Planorabarius corneus snail Acanthaceae Azinphosmethyl Carboxylesterase Chlorpyrifos Cholinesterase Inhibitors Cholinesterases Drug Synergism Oxygen Pesticides Gastropoda Invertebrata Planorbarius corneus In this study, the cholinesterase (ChE) and carboxylesterase (CES) activities present in whole organism homogenates from Planorbarius corneus and their in vitro sensitivity to organophosphorous (OP) pesticides were studied. Firstly, a characterization of ChE and CES activities using different substrates and selective inhibitors was performed. Secondly, the effects of azinphos-methyl oxon (AZM-oxon) and chlorpyrifos oxon (CPF-oxon), the active oxygen analogs of the OP insecticides AZM and CPF, on ChE and CES activities were evaluated. Finally, it was analyzed whether binary mixtures of the pesticide oxons cause additive, antagonistic or synergistic ChE inhibition in P. corneus homogenates. The results showed that the extracts of P. corneus preferentially hydrolyzed acetylthiocholine (AcSCh) over propionylthiocholine (PrSCh) and butyrylthiocholine (BuSCh). Besides, AcSCh hydrolyzing activity was inhibited by low concentrations of BW284c51, a selective inhibitor of AChE activity, and also by high concentrations of substrate. These facts suggest the presence of a typical AChE activity in this species. However, the different dose-response curves observed with BW284c51 when using PrSCh or BuSCh instead of AcSCh suggest the presence of at least another ChE activity. This would probably correspond to an atypical BuChE. Regarding CES activity, the highest specific activity was obtained when using 2-naphthyl acetate (2-NA), followed by 1-naphthyl acetate (1-NA); p-nitrophenyl acetate (p-NPA), and p-nitrophenyl butyrate (p-NPB). The comparison of the IC50 values revealed that, regardless of the substrate used, CES activity was approximately one order of magnitude more sensitive to AZM-oxon than ChE activity. Although ChE activity was very sensitive to CPF-oxon, CES activity measured with 1-NA, 2-NA, and p-NPA was poorly inhibited by this pesticide. In contrast, CES activity measured with p-NPB was equally sensitive to CPF-oxon than ChE activity. Several specific binary combinations of AZM-oxon and CPF-oxon caused a synergistic effect on the ChE inhibition in P. corneus homogenates. The degree of synergism tended to increase as the ratio of AZM-oxon to CPF-oxon decreased. These results suggest that synergism is likely to occur in P. corneus snails exposed in vivo to binary mixtures of the OPs AZM and CPF. © 2012 Elsevier Ltd. Fil:Kristoff, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Verrengia Guerrero, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cochón, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00456535_v88_n4_p450_Cacciatore http://hdl.handle.net/20.500.12110/paper_00456535_v88_n4_p450_Cacciatore |