Nitric oxide and HSV vaginal infection in BALB/c mice
Here we study the role of nitric oxide in the vaginal infection of Balb/c mice with herpes simplex virus type 2. Inducible nitric oxide synthase (iNOS) mRNA was detected by RT-PCR in vaginal tissue and inguinal lymph nodes early postinfection. iNOS was also found to be activated in cells recovered f...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00426822_v309_n1_p75_Benencia http://hdl.handle.net/20.500.12110/paper_00426822_v309_n1_p75_Benencia |
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paper:paper_00426822_v309_n1_p75_Benencia2023-06-08T15:04:51Z Nitric oxide and HSV vaginal infection in BALB/c mice aminoguanidine macrophage inflammatory protein 2 messenger RNA n acetyl s nitrosopenicillamine nitric oxide nitric oxide donor nitric oxide synthase nitric oxide synthase inhibitor RANTES animal cell animal experiment animal model animal tissue article cell count controlled study enzyme activation exudate female Herpes simplex virus 2 histopathology inguinal lymph node mouse nonhuman polymorphonuclear cell priority journal protein expression reverse transcription polymerase chain reaction vaginitis virus replication virus titration Animalia Human herpesvirus 2 Simplexvirus Here we study the role of nitric oxide in the vaginal infection of Balb/c mice with herpes simplex virus type 2. Inducible nitric oxide synthase (iNOS) mRNA was detected by RT-PCR in vaginal tissue and inguinal lymph nodes early postinfection. iNOS was also found to be activated in cells recovered from vaginal washings of infected animals. Animals treated with aminoguanidine (AG), an iNOS inhibitor, showed a dose-dependent increase in vaginal pathology after viral infection compared to controls. Viral titers in vaginal washings and vaginas were higher in AG-treated mice. Treated animals presented higher PMN counts in vaginal washings compared to controls. Histopathology studies revealed a profound inflammatory exudate in vaginal tissue of treated animals. Finally, RT-PCR analysis showed increased expression of the chemokines MIP-2 and RANTES in vaginal tissue and inguinal lymph nodes of these animals. © 2003 Elsevier Science (USA). All rights reserved. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00426822_v309_n1_p75_Benencia http://hdl.handle.net/20.500.12110/paper_00426822_v309_n1_p75_Benencia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
aminoguanidine macrophage inflammatory protein 2 messenger RNA n acetyl s nitrosopenicillamine nitric oxide nitric oxide donor nitric oxide synthase nitric oxide synthase inhibitor RANTES animal cell animal experiment animal model animal tissue article cell count controlled study enzyme activation exudate female Herpes simplex virus 2 histopathology inguinal lymph node mouse nonhuman polymorphonuclear cell priority journal protein expression reverse transcription polymerase chain reaction vaginitis virus replication virus titration Animalia Human herpesvirus 2 Simplexvirus |
| spellingShingle |
aminoguanidine macrophage inflammatory protein 2 messenger RNA n acetyl s nitrosopenicillamine nitric oxide nitric oxide donor nitric oxide synthase nitric oxide synthase inhibitor RANTES animal cell animal experiment animal model animal tissue article cell count controlled study enzyme activation exudate female Herpes simplex virus 2 histopathology inguinal lymph node mouse nonhuman polymorphonuclear cell priority journal protein expression reverse transcription polymerase chain reaction vaginitis virus replication virus titration Animalia Human herpesvirus 2 Simplexvirus Nitric oxide and HSV vaginal infection in BALB/c mice |
| topic_facet |
aminoguanidine macrophage inflammatory protein 2 messenger RNA n acetyl s nitrosopenicillamine nitric oxide nitric oxide donor nitric oxide synthase nitric oxide synthase inhibitor RANTES animal cell animal experiment animal model animal tissue article cell count controlled study enzyme activation exudate female Herpes simplex virus 2 histopathology inguinal lymph node mouse nonhuman polymorphonuclear cell priority journal protein expression reverse transcription polymerase chain reaction vaginitis virus replication virus titration Animalia Human herpesvirus 2 Simplexvirus |
| description |
Here we study the role of nitric oxide in the vaginal infection of Balb/c mice with herpes simplex virus type 2. Inducible nitric oxide synthase (iNOS) mRNA was detected by RT-PCR in vaginal tissue and inguinal lymph nodes early postinfection. iNOS was also found to be activated in cells recovered from vaginal washings of infected animals. Animals treated with aminoguanidine (AG), an iNOS inhibitor, showed a dose-dependent increase in vaginal pathology after viral infection compared to controls. Viral titers in vaginal washings and vaginas were higher in AG-treated mice. Treated animals presented higher PMN counts in vaginal washings compared to controls. Histopathology studies revealed a profound inflammatory exudate in vaginal tissue of treated animals. Finally, RT-PCR analysis showed increased expression of the chemokines MIP-2 and RANTES in vaginal tissue and inguinal lymph nodes of these animals. © 2003 Elsevier Science (USA). All rights reserved. |
| title |
Nitric oxide and HSV vaginal infection in BALB/c mice |
| title_short |
Nitric oxide and HSV vaginal infection in BALB/c mice |
| title_full |
Nitric oxide and HSV vaginal infection in BALB/c mice |
| title_fullStr |
Nitric oxide and HSV vaginal infection in BALB/c mice |
| title_full_unstemmed |
Nitric oxide and HSV vaginal infection in BALB/c mice |
| title_sort |
nitric oxide and hsv vaginal infection in balb/c mice |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00426822_v309_n1_p75_Benencia http://hdl.handle.net/20.500.12110/paper_00426822_v309_n1_p75_Benencia |
| _version_ |
1768542212570742784 |