Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor and the first protein involved in a variety of physiological and toxicological processes, including those of xenobiotic metabolizing enzymes. AhR has been found in the ovary of many species and seems to mediate the ovaria...

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Autores principales:	Barreiro, Karina Andrea, Di Yorio, María Paula, Paz, Dante Agustin, Faletti, Alicia Graciela
Publicado:	2011
Materias:	α-naphthoflavone AhR Follicular growth Ovulation alpha naphthoflavone chorionic gonadotropin animal experiment animal tissue article corpus luteum drug mechanism female immunohistochemistry nonhuman oocyte organ weight ovary ovary follicle development oviduct ovulation rat Animals Benzoflavones Dose-Response Relationship, Drug Drug Administration Schedule Female Humans Ovarian Follicle Random Allocation Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Rattus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0041008X_v252_n1_p11_Barreiro http://hdl.handle.net/20.500.12110/paper_0041008X_v252_n1_p11_Barreiro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_0041008X_v252_n1_p11_Barreiro2023-06-08T15:04:43Z Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat Barreiro, Karina Andrea Di Yorio, María Paula Paz, Dante Agustin Faletti, Alicia Graciela α-naphthoflavone AhR Follicular growth Ovulation alpha naphthoflavone chorionic gonadotropin animal experiment animal tissue article corpus luteum drug mechanism female immunohistochemistry nonhuman oocyte organ weight ovary ovary follicle development oviduct ovulation rat Animals Benzoflavones Dose-Response Relationship, Drug Drug Administration Schedule Female Humans Ovarian Follicle Ovulation Random Allocation Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Rattus The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor and the first protein involved in a variety of physiological and toxicological processes, including those of xenobiotic metabolizing enzymes. AhR has been found in the ovary of many species and seems to mediate the ovarian toxicity of many environmental contaminants, which are AhR ligands. However, the role of AhR in the ovarian function is unknown. Therefore, the aim of this work was to study the action of α-naphthoflavone (αNF), known to be an AhR antagonist, on both follicular growth and ovulation. Immature Sprague-Dawley rats were daily injected intraperitoneally with αNF (0.1-80. mg/kg) or vehicle for 12. days, and primed with gonadotrophins (eCG/hCG) to induce follicular growth and ovulation. Ovaries were obtained 20. h after hCG administration. By means of immunohistochemistry, we found that the numbers of primordial, primary and antral follicles were increased in rats treated with 80. mg/kg αNF and that there were no differences with other doses. Likewise, the ovarian weight and the ovulation rate, measured by both number of oocytes within oviducts and corpora lutea in ovarian sections, were increased when the rats received either 1 or 10. mg/kg daily. Although further studies are necessary to know the mechanism of action of αNF, it is possible that the different ovarian processes can be differentially responsive to the presence of different levels of αNF, and that the same or different endogenous AhR ligands can be involved in these ovarian processes in a cell type-dependent manner. © 2011 Elsevier Inc. Fil:Barreiro, K.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0041008X_v252_n1_p11_Barreiro http://hdl.handle.net/20.500.12110/paper_0041008X_v252_n1_p11_Barreiro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	α-naphthoflavone AhR Follicular growth Ovulation alpha naphthoflavone chorionic gonadotropin animal experiment animal tissue article corpus luteum drug mechanism female immunohistochemistry nonhuman oocyte organ weight ovary ovary follicle development oviduct ovulation rat Animals Benzoflavones Dose-Response Relationship, Drug Drug Administration Schedule Female Humans Ovarian Follicle Ovulation Random Allocation Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Rattus
spellingShingle	α-naphthoflavone AhR Follicular growth Ovulation alpha naphthoflavone chorionic gonadotropin animal experiment animal tissue article corpus luteum drug mechanism female immunohistochemistry nonhuman oocyte organ weight ovary ovary follicle development oviduct ovulation rat Animals Benzoflavones Dose-Response Relationship, Drug Drug Administration Schedule Female Humans Ovarian Follicle Ovulation Random Allocation Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Rattus Barreiro, Karina Andrea Di Yorio, María Paula Paz, Dante Agustin Faletti, Alicia Graciela Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat
topic_facet	α-naphthoflavone AhR Follicular growth Ovulation alpha naphthoflavone chorionic gonadotropin animal experiment animal tissue article corpus luteum drug mechanism female immunohistochemistry nonhuman oocyte organ weight ovary ovary follicle development oviduct ovulation rat Animals Benzoflavones Dose-Response Relationship, Drug Drug Administration Schedule Female Humans Ovarian Follicle Ovulation Random Allocation Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Rattus
description	The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor and the first protein involved in a variety of physiological and toxicological processes, including those of xenobiotic metabolizing enzymes. AhR has been found in the ovary of many species and seems to mediate the ovarian toxicity of many environmental contaminants, which are AhR ligands. However, the role of AhR in the ovarian function is unknown. Therefore, the aim of this work was to study the action of α-naphthoflavone (αNF), known to be an AhR antagonist, on both follicular growth and ovulation. Immature Sprague-Dawley rats were daily injected intraperitoneally with αNF (0.1-80. mg/kg) or vehicle for 12. days, and primed with gonadotrophins (eCG/hCG) to induce follicular growth and ovulation. Ovaries were obtained 20. h after hCG administration. By means of immunohistochemistry, we found that the numbers of primordial, primary and antral follicles were increased in rats treated with 80. mg/kg αNF and that there were no differences with other doses. Likewise, the ovarian weight and the ovulation rate, measured by both number of oocytes within oviducts and corpora lutea in ovarian sections, were increased when the rats received either 1 or 10. mg/kg daily. Although further studies are necessary to know the mechanism of action of αNF, it is possible that the different ovarian processes can be differentially responsive to the presence of different levels of αNF, and that the same or different endogenous AhR ligands can be involved in these ovarian processes in a cell type-dependent manner. © 2011 Elsevier Inc.
author	Barreiro, Karina Andrea Di Yorio, María Paula Paz, Dante Agustin Faletti, Alicia Graciela
author_facet	Barreiro, Karina Andrea Di Yorio, María Paula Paz, Dante Agustin Faletti, Alicia Graciela
author_sort	Barreiro, Karina Andrea
title	Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat
title_short	Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat
title_full	Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat
title_fullStr	Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat
title_full_unstemmed	Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat
title_sort	daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0041008X_v252_n1_p11_Barreiro http://hdl.handle.net/20.500.12110/paper_0041008X_v252_n1_p11_Barreiro
work_keys_str_mv	AT barreirokarinaandrea dailytreatmentwithanaphthoflavoneenhancesfolliculargrowthandovulationrateintherat AT diyoriomariapaula dailytreatmentwithanaphthoflavoneenhancesfolliculargrowthandovulationrateintherat AT pazdanteagustin dailytreatmentwithanaphthoflavoneenhancesfolliculargrowthandovulationrateintherat AT falettialiciagraciela dailytreatmentwithanaphthoflavoneenhancesfolliculargrowthandovulationrateintherat
_version_	1768544722579619840

Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat

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