Immunocytochemical distribution of corticotropin-releasing factor in the brain and hypophysis of larval Bufo arenarum; Effect of KClO4 during early development
The maturation of the corticotropin-releasing factor (CRF) neuronal system was evaluated by immunocytochemistry and morphometry in Bufo arenarum, during spontaneous metamorphosis and in tadpoles with inhibited thryroid function. The first appearance of CRF immunoreactive fibers was at the end of pre...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00408166_v29_n6_p643_Miranda http://hdl.handle.net/20.500.12110/paper_00408166_v29_n6_p643_Miranda |
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paper:paper_00408166_v29_n6_p643_Miranda2023-06-08T15:04:39Z Immunocytochemical distribution of corticotropin-releasing factor in the brain and hypophysis of larval Bufo arenarum; Effect of KClO4 during early development Amphibia Brain Corticotropin-releasing factor Potassium perchlorate Thyroid hormones corticotropin releasing factor potassium perchlorate steroid hormone thyroid hormone animal cell animal tissue article brain hormone synthesis hypophysis immunocytochemistry interpeduncular nucleus larval development median eminence metamorphosis morphometrics negative feedback nonhuman tadpole thyroid function toad The maturation of the corticotropin-releasing factor (CRF) neuronal system was evaluated by immunocytochemistry and morphometry in Bufo arenarum, during spontaneous metamorphosis and in tadpoles with inhibited thryroid function. The first appearance of CRF immunoreactive fibers was at the end of premetamorphosis (stage VIII). These fibers were found in small numbers and weakly stained in the median eminence and infundibular stalk. With the advance of larval development, CRF-like material stained intensely and tended to aggregate in the external zone of the median eminence. At climax stages, immunoreactive fibers and perikarya (weakly stained) were identified in the interpeduncular nucleus and in the dorsal infundibular nucleus. Morphometric and immunocytochemical results indicate that the maturation of the CRF neuronal system in Bufo arenarum occurs just before metamorphic climax, coinciding with high levels of thyroid and steroid hormones. We have also found that in larvae with inhibited thyroid function, the CRF neuronal system is able to develop, and that thyroid hormone could exert a negative feedback control on the synthesis of CRF. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00408166_v29_n6_p643_Miranda http://hdl.handle.net/20.500.12110/paper_00408166_v29_n6_p643_Miranda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amphibia Brain Corticotropin-releasing factor Potassium perchlorate Thyroid hormones corticotropin releasing factor potassium perchlorate steroid hormone thyroid hormone animal cell animal tissue article brain hormone synthesis hypophysis immunocytochemistry interpeduncular nucleus larval development median eminence metamorphosis morphometrics negative feedback nonhuman tadpole thyroid function toad |
| spellingShingle |
Amphibia Brain Corticotropin-releasing factor Potassium perchlorate Thyroid hormones corticotropin releasing factor potassium perchlorate steroid hormone thyroid hormone animal cell animal tissue article brain hormone synthesis hypophysis immunocytochemistry interpeduncular nucleus larval development median eminence metamorphosis morphometrics negative feedback nonhuman tadpole thyroid function toad Immunocytochemical distribution of corticotropin-releasing factor in the brain and hypophysis of larval Bufo arenarum; Effect of KClO4 during early development |
| topic_facet |
Amphibia Brain Corticotropin-releasing factor Potassium perchlorate Thyroid hormones corticotropin releasing factor potassium perchlorate steroid hormone thyroid hormone animal cell animal tissue article brain hormone synthesis hypophysis immunocytochemistry interpeduncular nucleus larval development median eminence metamorphosis morphometrics negative feedback nonhuman tadpole thyroid function toad |
| description |
The maturation of the corticotropin-releasing factor (CRF) neuronal system was evaluated by immunocytochemistry and morphometry in Bufo arenarum, during spontaneous metamorphosis and in tadpoles with inhibited thryroid function. The first appearance of CRF immunoreactive fibers was at the end of premetamorphosis (stage VIII). These fibers were found in small numbers and weakly stained in the median eminence and infundibular stalk. With the advance of larval development, CRF-like material stained intensely and tended to aggregate in the external zone of the median eminence. At climax stages, immunoreactive fibers and perikarya (weakly stained) were identified in the interpeduncular nucleus and in the dorsal infundibular nucleus. Morphometric and immunocytochemical results indicate that the maturation of the CRF neuronal system in Bufo arenarum occurs just before metamorphic climax, coinciding with high levels of thyroid and steroid hormones. We have also found that in larvae with inhibited thyroid function, the CRF neuronal system is able to develop, and that thyroid hormone could exert a negative feedback control on the synthesis of CRF. |
| title |
Immunocytochemical distribution of corticotropin-releasing factor in the brain and hypophysis of larval Bufo arenarum; Effect of KClO4 during early development |
| title_short |
Immunocytochemical distribution of corticotropin-releasing factor in the brain and hypophysis of larval Bufo arenarum; Effect of KClO4 during early development |
| title_full |
Immunocytochemical distribution of corticotropin-releasing factor in the brain and hypophysis of larval Bufo arenarum; Effect of KClO4 during early development |
| title_fullStr |
Immunocytochemical distribution of corticotropin-releasing factor in the brain and hypophysis of larval Bufo arenarum; Effect of KClO4 during early development |
| title_full_unstemmed |
Immunocytochemical distribution of corticotropin-releasing factor in the brain and hypophysis of larval Bufo arenarum; Effect of KClO4 during early development |
| title_sort |
immunocytochemical distribution of corticotropin-releasing factor in the brain and hypophysis of larval bufo arenarum; effect of kclo4 during early development |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00408166_v29_n6_p643_Miranda http://hdl.handle.net/20.500.12110/paper_00408166_v29_n6_p643_Miranda |
| _version_ |
1768542259162120192 |