Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor
The roles of chemical kinetics and mass transfer in three types of bioreactors (packed-column reactors, rotating disk bioreactors and amperometric detector), used with continuous-flow sample/reagent(s) processing, are discussed in detail. A normalized quantitative comparison between these types of r...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00399140_v66_n1_p92_Salinas http://hdl.handle.net/20.500.12110/paper_00399140_v66_n1_p92_Salinas |
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paper:paper_00399140_v66_n1_p92_Salinas2023-06-08T15:03:43Z Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor Flow system Horseradish peroxidase Osmium Packed-column reactors Rotating disk bioreactors Bioassay Biocatalysts Bioreactors Escherichia coli Hydrogen peroxide Immunosensors Osmium Oxidation Polymerization Cell growth Flow system Horseradish peroxidase Packed-column reactors Enzymes The roles of chemical kinetics and mass transfer in three types of bioreactors (packed-column reactors, rotating disk bioreactors and amperometric detector), used with continuous-flow sample/reagent(s) processing, are discussed in detail. A normalized quantitative comparison between these types of reactors clearly shows that rotating disk reactors afford a significantly more efficient utilization of active sites and permit the effective utilization of very small amounts of biocatalysts. Horseradish peroxidase (EC 1.11.1.7), in presence of hydrogen peroxide catalyses the oxidation of [Os(bpy)2Cl(pyCOOH)]Cl. The electrochemical reduction back of this cosubstrate is detected on glassy carbon electrode surface at 0.00 V. Furthermore, the critical effect of substrate and cosubstrate concentration on amperometric immunosensors construction in which HRP is used as an enzymatic label was studied. © 2004 Elsevier B.V. All rights reserved. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00399140_v66_n1_p92_Salinas http://hdl.handle.net/20.500.12110/paper_00399140_v66_n1_p92_Salinas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Flow system Horseradish peroxidase Osmium Packed-column reactors Rotating disk bioreactors Bioassay Biocatalysts Bioreactors Escherichia coli Hydrogen peroxide Immunosensors Osmium Oxidation Polymerization Cell growth Flow system Horseradish peroxidase Packed-column reactors Enzymes |
| spellingShingle |
Flow system Horseradish peroxidase Osmium Packed-column reactors Rotating disk bioreactors Bioassay Biocatalysts Bioreactors Escherichia coli Hydrogen peroxide Immunosensors Osmium Oxidation Polymerization Cell growth Flow system Horseradish peroxidase Packed-column reactors Enzymes Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor |
| topic_facet |
Flow system Horseradish peroxidase Osmium Packed-column reactors Rotating disk bioreactors Bioassay Biocatalysts Bioreactors Escherichia coli Hydrogen peroxide Immunosensors Osmium Oxidation Polymerization Cell growth Flow system Horseradish peroxidase Packed-column reactors Enzymes |
| description |
The roles of chemical kinetics and mass transfer in three types of bioreactors (packed-column reactors, rotating disk bioreactors and amperometric detector), used with continuous-flow sample/reagent(s) processing, are discussed in detail. A normalized quantitative comparison between these types of reactors clearly shows that rotating disk reactors afford a significantly more efficient utilization of active sites and permit the effective utilization of very small amounts of biocatalysts. Horseradish peroxidase (EC 1.11.1.7), in presence of hydrogen peroxide catalyses the oxidation of [Os(bpy)2Cl(pyCOOH)]Cl. The electrochemical reduction back of this cosubstrate is detected on glassy carbon electrode surface at 0.00 V. Furthermore, the critical effect of substrate and cosubstrate concentration on amperometric immunosensors construction in which HRP is used as an enzymatic label was studied. © 2004 Elsevier B.V. All rights reserved. |
| title |
Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor |
| title_short |
Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor |
| title_full |
Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor |
| title_fullStr |
Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor |
| title_full_unstemmed |
Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor |
| title_sort |
continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00399140_v66_n1_p92_Salinas http://hdl.handle.net/20.500.12110/paper_00399140_v66_n1_p92_Salinas |
| _version_ |
1768545823448104960 |