Endothelin-1 -induced incorporation of cholesterol into rat adrenals

The effect of endothelin-1 (ET-1) on cholesterol uptake by adrenal cortex was evaluated through several experimental approaches: infusion of ET-1 followed by measurement of endogenous cholesterol in excised adrenals; infusion of ET-1 followed by tritiated cholesterol incorporation into adrenal quart...

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Autores principales:	Romero, Damián Gastón, Pecci, Adali, Cozza, Eduardo Néstor
Publicado:	1996
Materias:	Adrenal Cholesterol Endothelin Steroidogenesis 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide cholesterol cholesterol monooxygenase (side chain cleaving) endothelin 1 endothelin receptor isomerase tritium adrenal cell adrenal cortex animal cell animal experiment animal tissue article cell culture cell fractionation cholesterol metabolism cholesterol transport controlled study enzyme activity infusion isotope labeling male nonhuman radioactivity rat Adrenal Glands Aldosterone Aminoglutethimide Animals Endothelin-1 Infusions, Intravenous Male Rats Rats, Inbred Strains Animalia Tritium
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v61_n5_p317_Romero http://hdl.handle.net/20.500.12110/paper_0039128X_v61_n5_p317_Romero
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0039128X_v61_n5_p317_Romero
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spelling	paper:paper_0039128X_v61_n5_p317_Romero2023-06-08T15:03:16Z Endothelin-1 -induced incorporation of cholesterol into rat adrenals Romero, Damián Gastón Pecci, Adali Cozza, Eduardo Néstor Adrenal Cholesterol Endothelin Steroidogenesis 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide cholesterol cholesterol monooxygenase (side chain cleaving) endothelin 1 endothelin receptor isomerase tritium adrenal cell adrenal cortex animal cell animal experiment animal tissue article cell culture cell fractionation cholesterol metabolism cholesterol transport controlled study enzyme activity infusion isotope labeling male nonhuman radioactivity rat Adrenal Glands Aldosterone Aminoglutethimide Animals Cholesterol Endothelin-1 Infusions, Intravenous Male Rats Rats, Inbred Strains Animalia Tritium The effect of endothelin-1 (ET-1) on cholesterol uptake by adrenal cortex was evaluated through several experimental approaches: infusion of ET-1 followed by measurement of endogenous cholesterol in excised adrenals; infusion of ET-1 followed by tritiated cholesterol incorporation into adrenal quarters in vitro; coinfusion of ET-1 with tritiated cholesterol-enriched serum and determination of adrenal-associated radioactivity; and tritiated cholesterol incorporation in incubations of adrenal cells. In all cases ET-1 increased cholesterol uptake. Subcellular fractionation showed an ET-1-mediated augmentation in mitochondrial fraction. This increase was mediated by the subpopulation B of adrenal receptors for ET-1. In addition, ET-1 also increased cytochrome P450-SCC (side-chain cleavage) activity. © 1996 by Elsevier Science Inc. Fil:Romero, D.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v61_n5_p317_Romero http://hdl.handle.net/20.500.12110/paper_0039128X_v61_n5_p317_Romero
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adrenal Cholesterol Endothelin Steroidogenesis 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide cholesterol cholesterol monooxygenase (side chain cleaving) endothelin 1 endothelin receptor isomerase tritium adrenal cell adrenal cortex animal cell animal experiment animal tissue article cell culture cell fractionation cholesterol metabolism cholesterol transport controlled study enzyme activity infusion isotope labeling male nonhuman radioactivity rat Adrenal Glands Aldosterone Aminoglutethimide Animals Cholesterol Endothelin-1 Infusions, Intravenous Male Rats Rats, Inbred Strains Animalia Tritium
spellingShingle	Adrenal Cholesterol Endothelin Steroidogenesis 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide cholesterol cholesterol monooxygenase (side chain cleaving) endothelin 1 endothelin receptor isomerase tritium adrenal cell adrenal cortex animal cell animal experiment animal tissue article cell culture cell fractionation cholesterol metabolism cholesterol transport controlled study enzyme activity infusion isotope labeling male nonhuman radioactivity rat Adrenal Glands Aldosterone Aminoglutethimide Animals Cholesterol Endothelin-1 Infusions, Intravenous Male Rats Rats, Inbred Strains Animalia Tritium Romero, Damián Gastón Pecci, Adali Cozza, Eduardo Néstor Endothelin-1 -induced incorporation of cholesterol into rat adrenals
topic_facet	Adrenal Cholesterol Endothelin Steroidogenesis 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide cholesterol cholesterol monooxygenase (side chain cleaving) endothelin 1 endothelin receptor isomerase tritium adrenal cell adrenal cortex animal cell animal experiment animal tissue article cell culture cell fractionation cholesterol metabolism cholesterol transport controlled study enzyme activity infusion isotope labeling male nonhuman radioactivity rat Adrenal Glands Aldosterone Aminoglutethimide Animals Cholesterol Endothelin-1 Infusions, Intravenous Male Rats Rats, Inbred Strains Animalia Tritium
description	The effect of endothelin-1 (ET-1) on cholesterol uptake by adrenal cortex was evaluated through several experimental approaches: infusion of ET-1 followed by measurement of endogenous cholesterol in excised adrenals; infusion of ET-1 followed by tritiated cholesterol incorporation into adrenal quarters in vitro; coinfusion of ET-1 with tritiated cholesterol-enriched serum and determination of adrenal-associated radioactivity; and tritiated cholesterol incorporation in incubations of adrenal cells. In all cases ET-1 increased cholesterol uptake. Subcellular fractionation showed an ET-1-mediated augmentation in mitochondrial fraction. This increase was mediated by the subpopulation B of adrenal receptors for ET-1. In addition, ET-1 also increased cytochrome P450-SCC (side-chain cleavage) activity. © 1996 by Elsevier Science Inc.
author	Romero, Damián Gastón Pecci, Adali Cozza, Eduardo Néstor
author_facet	Romero, Damián Gastón Pecci, Adali Cozza, Eduardo Néstor
author_sort	Romero, Damián Gastón
title	Endothelin-1 -induced incorporation of cholesterol into rat adrenals
title_short	Endothelin-1 -induced incorporation of cholesterol into rat adrenals
title_full	Endothelin-1 -induced incorporation of cholesterol into rat adrenals
title_fullStr	Endothelin-1 -induced incorporation of cholesterol into rat adrenals
title_full_unstemmed	Endothelin-1 -induced incorporation of cholesterol into rat adrenals
title_sort	endothelin-1 -induced incorporation of cholesterol into rat adrenals
publishDate	1996
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v61_n5_p317_Romero http://hdl.handle.net/20.500.12110/paper_0039128X_v61_n5_p317_Romero
work_keys_str_mv	AT romerodamiangaston endothelin1inducedincorporationofcholesterolintoratadrenals AT pecciadali endothelin1inducedincorporationofcholesterolintoratadrenals AT cozzaeduardonestor endothelin1inducedincorporationofcholesterolintoratadrenals
_version_	1768545225244934144

Endothelin-1 -induced incorporation of cholesterol into rat adrenals

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