Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria

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In this work we confirm by a metabolic method the existence of at least two enzymes with 11β- and 18-hydroxylase activities in rat adrenal mitochondria. The method was based on the ability of cortisol (F), a foreign alternative substrate, to inhibit competitively metabolite productions from various...

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Autor principal:	Cozza, Eduardo Néstor
Publicado:	1995
Materias:	11β, 18-hydroxylases adrenal aldosterone Cortisol cytochrome P450 steroidogenesis 18 hydroxycorticosterone 18 hydroxydeoxycorticosterone cytochrome p450 deoxycorticosterone hydrocortisone mitochondrial enzyme oxygenase steroid 11beta monooxygenase aldosterone release animal tissue article enzyme activity enzyme localization enzyme substrate male mitochondrion molecular cloning nonhuman rat zona glomerulosa 18-Hydroxycorticosterone Adrenal Glands Aldosterone Aldosterone Antagonists Animal Desoxycorticosterone Hydrocortisone Male Mitochondria Mixed Function Oxygenases Rats Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Animalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v60_n6_p447_Matkovic http://hdl.handle.net/20.500.12110/paper_0039128X_v60_n6_p447_Matkovic
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_0039128X_v60_n6_p447_Matkovic2023-06-08T15:03:15Z Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria Cozza, Eduardo Néstor 11β, 18-hydroxylases adrenal aldosterone Cortisol cytochrome P450 steroidogenesis 18 hydroxycorticosterone 18 hydroxydeoxycorticosterone aldosterone cytochrome p450 deoxycorticosterone hydrocortisone mitochondrial enzyme oxygenase steroid 11beta monooxygenase aldosterone release animal tissue article enzyme activity enzyme localization enzyme substrate male mitochondrion molecular cloning nonhuman rat steroidogenesis zona glomerulosa 18-Hydroxycorticosterone Adrenal Glands Aldosterone Aldosterone Antagonists Animal Desoxycorticosterone Hydrocortisone Male Mitochondria Mixed Function Oxygenases Rats Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Animalia In this work we confirm by a metabolic method the existence of at least two enzymes with 11β- and 18-hydroxylase activities in rat adrenal mitochondria. The method was based on the ability of cortisol (F), a foreign alternative substrate, to inhibit competitively metabolite productions from various precursors. F inhibited a) aldosterone (ALDO) production from 11-deoxycorticosterone (DOC) without affecting the yields of corticosterone (B) and 18-hydroxy-11-deoxycorticosterone (18-OHDOC); b) 18-hydroxycorticosterone and aldosterone productions from B (Ki = 2.5 ± 0.5 μM); and c) ALDO production from 18-OHDOC. These results suggest the existence of two categories of enzymes with both 11β- and 18-hydroxylase activities, one comprising those that catalyze the conversions of DOC to B and 18-OHDOC (F-insensitive reactions [FIS]) and the other one comprising the enzymes involved in the conversions of B to 18-OHB and ALDO and that of 18-OHDOC to ALDO (F-sensitive reactions [FS]). The cloned enzymes CYP11B1 and CYP11B2 would pertain respectively to the FIS and FS categories. © 1995. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v60_n6_p447_Matkovic http://hdl.handle.net/20.500.12110/paper_0039128X_v60_n6_p447_Matkovic
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	11β, 18-hydroxylases adrenal aldosterone Cortisol cytochrome P450 steroidogenesis 18 hydroxycorticosterone 18 hydroxydeoxycorticosterone aldosterone cytochrome p450 deoxycorticosterone hydrocortisone mitochondrial enzyme oxygenase steroid 11beta monooxygenase aldosterone release animal tissue article enzyme activity enzyme localization enzyme substrate male mitochondrion molecular cloning nonhuman rat steroidogenesis zona glomerulosa 18-Hydroxycorticosterone Adrenal Glands Aldosterone Aldosterone Antagonists Animal Desoxycorticosterone Hydrocortisone Male Mitochondria Mixed Function Oxygenases Rats Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Animalia
spellingShingle	11β, 18-hydroxylases adrenal aldosterone Cortisol cytochrome P450 steroidogenesis 18 hydroxycorticosterone 18 hydroxydeoxycorticosterone aldosterone cytochrome p450 deoxycorticosterone hydrocortisone mitochondrial enzyme oxygenase steroid 11beta monooxygenase aldosterone release animal tissue article enzyme activity enzyme localization enzyme substrate male mitochondrion molecular cloning nonhuman rat steroidogenesis zona glomerulosa 18-Hydroxycorticosterone Adrenal Glands Aldosterone Aldosterone Antagonists Animal Desoxycorticosterone Hydrocortisone Male Mitochondria Mixed Function Oxygenases Rats Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Animalia Cozza, Eduardo Néstor Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria
topic_facet	11β, 18-hydroxylases adrenal aldosterone Cortisol cytochrome P450 steroidogenesis 18 hydroxycorticosterone 18 hydroxydeoxycorticosterone aldosterone cytochrome p450 deoxycorticosterone hydrocortisone mitochondrial enzyme oxygenase steroid 11beta monooxygenase aldosterone release animal tissue article enzyme activity enzyme localization enzyme substrate male mitochondrion molecular cloning nonhuman rat steroidogenesis zona glomerulosa 18-Hydroxycorticosterone Adrenal Glands Aldosterone Aldosterone Antagonists Animal Desoxycorticosterone Hydrocortisone Male Mitochondria Mixed Function Oxygenases Rats Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Animalia
description	In this work we confirm by a metabolic method the existence of at least two enzymes with 11β- and 18-hydroxylase activities in rat adrenal mitochondria. The method was based on the ability of cortisol (F), a foreign alternative substrate, to inhibit competitively metabolite productions from various precursors. F inhibited a) aldosterone (ALDO) production from 11-deoxycorticosterone (DOC) without affecting the yields of corticosterone (B) and 18-hydroxy-11-deoxycorticosterone (18-OHDOC); b) 18-hydroxycorticosterone and aldosterone productions from B (Ki = 2.5 ± 0.5 μM); and c) ALDO production from 18-OHDOC. These results suggest the existence of two categories of enzymes with both 11β- and 18-hydroxylase activities, one comprising those that catalyze the conversions of DOC to B and 18-OHDOC (F-insensitive reactions [FIS]) and the other one comprising the enzymes involved in the conversions of B to 18-OHB and ALDO and that of 18-OHDOC to ALDO (F-sensitive reactions [FS]). The cloned enzymes CYP11B1 and CYP11B2 would pertain respectively to the FIS and FS categories. © 1995.
author	Cozza, Eduardo Néstor
author_facet	Cozza, Eduardo Néstor
author_sort	Cozza, Eduardo Néstor
title	Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria
title_short	Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria
title_full	Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria
title_fullStr	Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria
title_full_unstemmed	Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria
title_sort	inhibition of aldosterone formation by cortisol in rat adrenal mitochondria
publishDate	1995
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v60_n6_p447_Matkovic http://hdl.handle.net/20.500.12110/paper_0039128X_v60_n6_p447_Matkovic
work_keys_str_mv	AT cozzaeduardonestor inhibitionofaldosteroneformationbycortisolinratadrenalmitochondria
_version_	1768544495514681344

Inhibition of aldosterone formation by cortisol in rat adrenal mitochondria

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