A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production

Abstract Recent studies have shown that low concentrations of brassinolide induce a rapid generation of nitric oxide in mesophyll cells of maize leaves, which can be easily detected by fluorimetric methods. In this work we describe a series of natural and synthetic brassinosteroids that are able to...

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Autores principales:	Acebedo, Sofìa Lorena, Ramirez, Javier Alberto
Publicado:	2015
Materias:	Bioactivity assay Brassinosteroids Nitric oxide brassinosteroid nitric oxide brassinolide heterocyclic steroid analytic method Article bioassay controlled study fluorometry in vitro study intermethod comparison maize mesophyll cell nonhuman plant leaf tomato cytology metabolism plant cell procedures Lycopersicon esculentum Zea mays Biological Assay Fluorometry Nitric Oxide Plant Cells Plant Leaves Steroids, Heterocyclic
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v102_n_p46_Tossi http://hdl.handle.net/20.500.12110/paper_0039128X_v102_n_p46_Tossi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0039128X_v102_n_p46_Tossi
record_format	dspace
spelling	paper:paper_0039128X_v102_n_p46_Tossi2023-06-08T15:03:11Z A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production Acebedo, Sofìa Lorena Ramirez, Javier Alberto Bioactivity assay Brassinosteroids Nitric oxide brassinosteroid nitric oxide brassinolide brassinosteroid heterocyclic steroid nitric oxide analytic method Article bioassay controlled study fluorometry in vitro study intermethod comparison maize mesophyll cell nonhuman plant leaf tomato bioassay cytology fluorometry metabolism plant cell procedures Lycopersicon esculentum Zea mays Biological Assay Brassinosteroids Fluorometry Nitric Oxide Plant Cells Plant Leaves Steroids, Heterocyclic Zea mays Abstract Recent studies have shown that low concentrations of brassinolide induce a rapid generation of nitric oxide in mesophyll cells of maize leaves, which can be easily detected by fluorimetric methods. In this work we describe a series of natural and synthetic brassinosteroids that are able to trigger in vitro NO production in tomato cells that exhibits dose-response behavior. We propose that this effect can be used to develop a new rapid and very sensitive bioassay for brassinosteroid activity that offers several advantages when compared to the current methodologies. © 2015 Elsevier Inc. Fil:Acebedo, S.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramírez, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v102_n_p46_Tossi http://hdl.handle.net/20.500.12110/paper_0039128X_v102_n_p46_Tossi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bioactivity assay Brassinosteroids Nitric oxide brassinosteroid nitric oxide brassinolide brassinosteroid heterocyclic steroid nitric oxide analytic method Article bioassay controlled study fluorometry in vitro study intermethod comparison maize mesophyll cell nonhuman plant leaf tomato bioassay cytology fluorometry metabolism plant cell procedures Lycopersicon esculentum Zea mays Biological Assay Brassinosteroids Fluorometry Nitric Oxide Plant Cells Plant Leaves Steroids, Heterocyclic Zea mays
spellingShingle	Bioactivity assay Brassinosteroids Nitric oxide brassinosteroid nitric oxide brassinolide brassinosteroid heterocyclic steroid nitric oxide analytic method Article bioassay controlled study fluorometry in vitro study intermethod comparison maize mesophyll cell nonhuman plant leaf tomato bioassay cytology fluorometry metabolism plant cell procedures Lycopersicon esculentum Zea mays Biological Assay Brassinosteroids Fluorometry Nitric Oxide Plant Cells Plant Leaves Steroids, Heterocyclic Zea mays Acebedo, Sofìa Lorena Ramirez, Javier Alberto A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production
topic_facet	Bioactivity assay Brassinosteroids Nitric oxide brassinosteroid nitric oxide brassinolide brassinosteroid heterocyclic steroid nitric oxide analytic method Article bioassay controlled study fluorometry in vitro study intermethod comparison maize mesophyll cell nonhuman plant leaf tomato bioassay cytology fluorometry metabolism plant cell procedures Lycopersicon esculentum Zea mays Biological Assay Brassinosteroids Fluorometry Nitric Oxide Plant Cells Plant Leaves Steroids, Heterocyclic Zea mays
description	Abstract Recent studies have shown that low concentrations of brassinolide induce a rapid generation of nitric oxide in mesophyll cells of maize leaves, which can be easily detected by fluorimetric methods. In this work we describe a series of natural and synthetic brassinosteroids that are able to trigger in vitro NO production in tomato cells that exhibits dose-response behavior. We propose that this effect can be used to develop a new rapid and very sensitive bioassay for brassinosteroid activity that offers several advantages when compared to the current methodologies. © 2015 Elsevier Inc.
author	Acebedo, Sofìa Lorena Ramirez, Javier Alberto
author_facet	Acebedo, Sofìa Lorena Ramirez, Javier Alberto
author_sort	Acebedo, Sofìa Lorena
title	A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production
title_short	A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production
title_full	A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production
title_fullStr	A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production
title_full_unstemmed	A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production
title_sort	bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v102_n_p46_Tossi http://hdl.handle.net/20.500.12110/paper_0039128X_v102_n_p46_Tossi
work_keys_str_mv	AT acebedosofialorena abioassayforbrassinosteroidactivitybasedontheinvitrofluorimetricdetectionofnitricoxideproduction AT ramirezjavieralberto abioassayforbrassinosteroidactivitybasedontheinvitrofluorimetricdetectionofnitricoxideproduction AT acebedosofialorena bioassayforbrassinosteroidactivitybasedontheinvitrofluorimetricdetectionofnitricoxideproduction AT ramirezjavieralberto bioassayforbrassinosteroidactivitybasedontheinvitrofluorimetricdetectionofnitricoxideproduction
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A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production

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