α-Difluoromethylornithine modifies FSH secretion and puberty onset in the female rat (43954)
FSH secretion is high in immature female rats from Postnatal Day 5 to 18 and decreases thereafter. This is a relatively steroid-independent event of cerebral origin and of importance for puberty onset. Polyamines, a group of ubiquitous amines, play an essential role in tissue growth and differentiat...
Guardado en:
| Publicado: |
1996
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v211_n1_p76_Thyssen http://hdl.handle.net/20.500.12110/paper_00379727_v211_n1_p76_Thyssen |
| Aporte de: |
| id |
paper:paper_00379727_v211_n1_p76_Thyssen |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00379727_v211_n1_p76_Thyssen2023-06-08T15:02:24Z α-Difluoromethylornithine modifies FSH secretion and puberty onset in the female rat (43954) eflornithine animal experiment article brain development controlled study estrus cycle female fertility follitropin blood level follitropin release nonhuman puberty rat reproduction Animals Body Weight Eflornithine Enzyme Inhibitors Female Follicle Stimulating Hormone Ornithine Decarboxylase Prolactin Rats Rats, Sprague-Dawley Sexual Maturation Animalia FSH secretion is high in immature female rats from Postnatal Day 5 to 18 and decreases thereafter. This is a relatively steroid-independent event of cerebral origin and of importance for puberty onset. Polyamines, a group of ubiquitous amines, play an essential role in tissue growth and differentiation, body weight increment, brain organization, and molecular mechanisms of hormonal action. Polyamine levels as well as the activity of ornithine decarboxylase, the limiting enzyme in polyamines biosynthesis, are highest during development. Inhibition of their synthesis during this period by α-difluoromethylornithine (DFMO), a specific and irreversible inhibitor of ornithine decarboxylase, impairs normal brain development. The present study tested the hypothesis that polyamines play a role during brain organization of reproduction. DFMO was administered following different schedules in female newborn rats, and the effect on pituitary secretion, puberty onset, and fertility was evaluated. In three groups (daily injections from Day 1 to 9, or from Day 1 to 6, or injections on alternate days from Day 1 to 9), a delay in vaginal opening and first estrous was observed. When vaginal opening was plotted against body weight, it was evident that in groups daily injected with DFMO vaginal opening occurred at a lower body weight. In the group treated on alternate days, a delay occurred but at a higher body weight than in controls. In this group, serum FSH levels on Day 10 and 20, but not on Day 30, were higher in DFMO rats. In the group treated from Day 1 to 6 daily, DFMO Increased serum FSH on Postnatal Day 20. After vaginal opening, estrous cyclicity in control and DFMO injected rats was similar. There was no significant effect of treatment on fertility and litter weight or number of offspring at birth. It is concluded that DFMO, an inhibitor of ornithine decarboxylase, administered during the first week of life in female rats is followed by prolonged high FSH serum levels and delayed puberty, but once puberty occurs, fertility is normal. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v211_n1_p76_Thyssen http://hdl.handle.net/20.500.12110/paper_00379727_v211_n1_p76_Thyssen |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
eflornithine animal experiment article brain development controlled study estrus cycle female fertility follitropin blood level follitropin release nonhuman puberty rat reproduction Animals Body Weight Eflornithine Enzyme Inhibitors Female Follicle Stimulating Hormone Ornithine Decarboxylase Prolactin Rats Rats, Sprague-Dawley Sexual Maturation Animalia |
| spellingShingle |
eflornithine animal experiment article brain development controlled study estrus cycle female fertility follitropin blood level follitropin release nonhuman puberty rat reproduction Animals Body Weight Eflornithine Enzyme Inhibitors Female Follicle Stimulating Hormone Ornithine Decarboxylase Prolactin Rats Rats, Sprague-Dawley Sexual Maturation Animalia α-Difluoromethylornithine modifies FSH secretion and puberty onset in the female rat (43954) |
| topic_facet |
eflornithine animal experiment article brain development controlled study estrus cycle female fertility follitropin blood level follitropin release nonhuman puberty rat reproduction Animals Body Weight Eflornithine Enzyme Inhibitors Female Follicle Stimulating Hormone Ornithine Decarboxylase Prolactin Rats Rats, Sprague-Dawley Sexual Maturation Animalia |
| description |
FSH secretion is high in immature female rats from Postnatal Day 5 to 18 and decreases thereafter. This is a relatively steroid-independent event of cerebral origin and of importance for puberty onset. Polyamines, a group of ubiquitous amines, play an essential role in tissue growth and differentiation, body weight increment, brain organization, and molecular mechanisms of hormonal action. Polyamine levels as well as the activity of ornithine decarboxylase, the limiting enzyme in polyamines biosynthesis, are highest during development. Inhibition of their synthesis during this period by α-difluoromethylornithine (DFMO), a specific and irreversible inhibitor of ornithine decarboxylase, impairs normal brain development. The present study tested the hypothesis that polyamines play a role during brain organization of reproduction. DFMO was administered following different schedules in female newborn rats, and the effect on pituitary secretion, puberty onset, and fertility was evaluated. In three groups (daily injections from Day 1 to 9, or from Day 1 to 6, or injections on alternate days from Day 1 to 9), a delay in vaginal opening and first estrous was observed. When vaginal opening was plotted against body weight, it was evident that in groups daily injected with DFMO vaginal opening occurred at a lower body weight. In the group treated on alternate days, a delay occurred but at a higher body weight than in controls. In this group, serum FSH levels on Day 10 and 20, but not on Day 30, were higher in DFMO rats. In the group treated from Day 1 to 6 daily, DFMO Increased serum FSH on Postnatal Day 20. After vaginal opening, estrous cyclicity in control and DFMO injected rats was similar. There was no significant effect of treatment on fertility and litter weight or number of offspring at birth. It is concluded that DFMO, an inhibitor of ornithine decarboxylase, administered during the first week of life in female rats is followed by prolonged high FSH serum levels and delayed puberty, but once puberty occurs, fertility is normal. |
| title |
α-Difluoromethylornithine modifies FSH secretion and puberty onset in the female rat (43954) |
| title_short |
α-Difluoromethylornithine modifies FSH secretion and puberty onset in the female rat (43954) |
| title_full |
α-Difluoromethylornithine modifies FSH secretion and puberty onset in the female rat (43954) |
| title_fullStr |
α-Difluoromethylornithine modifies FSH secretion and puberty onset in the female rat (43954) |
| title_full_unstemmed |
α-Difluoromethylornithine modifies FSH secretion and puberty onset in the female rat (43954) |
| title_sort |
α-difluoromethylornithine modifies fsh secretion and puberty onset in the female rat (43954) |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v211_n1_p76_Thyssen http://hdl.handle.net/20.500.12110/paper_00379727_v211_n1_p76_Thyssen |
| _version_ |
1768543361298333696 |