O-glycosylated cell wall proteins are essential in root hair growth

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Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early...

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Autores principales:	Velasquez, Silvia Melina, Ricardi, Martiniano Maria, Fernández, Paula Virginia, Nadra, Alejandro Daniel, Ciancia, Marina, Iusem, Norberto Daniel, Estevez, Jose Manuel
Publicado:	2011
Materias:	arabinogalactan arabinosyltransferase cell membrane protein extensin glycan glycoprotein green fluorescent protein hydroxyproline rich glycoprotein procollagen proline 2 oxoglutarate 4 dioxygenase proline rich protein transferase unclassified drug adsorption dicotyledon enzyme activity hair inhibition polysaccharide protein Arabidopsis arabinosylation article cell growth cell wall chemical analysis chemical reaction glycosylation hydroxylation nonhuman priority journal protein expression protein function root hair Arabidopsis Proteins Arabinose Carbohydrate Conformation Cell Wall Gene Expression Regulation, Plant Genes, Plant Glycoproteins Glycosylation Glycosyltransferases Hydroxylation Hydroxyproline Models, Biological Mutation Pentosyltransferases Phenotype Plant Proteins Plant Roots Polysaccharides Procollagen-Proline Dioxygenase Proline Protein Conformation Protein Processing, Post-Translational Protein Structure, Secondary Arabidopsis thaliana
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00368075_v332_n6036_p1401_Velasquez http://hdl.handle.net/20.500.12110/paper_00368075_v332_n6036_p1401_Velasquez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00368075_v332_n6036_p1401_Velasquez
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spelling	paper:paper_00368075_v332_n6036_p1401_Velasquez2023-06-08T15:02:06Z O-glycosylated cell wall proteins are essential in root hair growth Velasquez, Silvia Melina Ricardi, Martiniano Maria Fernández, Paula Virginia Nadra, Alejandro Daniel Ciancia, Marina Iusem, Norberto Daniel Estevez, Jose Manuel arabinogalactan arabinosyltransferase cell membrane protein extensin glycan glycoprotein green fluorescent protein hydroxyproline rich glycoprotein procollagen proline 2 oxoglutarate 4 dioxygenase proline rich protein transferase unclassified drug adsorption dicotyledon enzyme activity hair inhibition polysaccharide protein Arabidopsis arabinosylation article cell growth cell wall chemical analysis chemical reaction glycosylation hydroxylation nonhuman priority journal protein expression protein function root hair Arabidopsis Arabidopsis Proteins Arabinose Carbohydrate Conformation Cell Wall Gene Expression Regulation, Plant Genes, Plant Glycoproteins Glycosylation Glycosyltransferases Hydroxylation Hydroxyproline Models, Biological Mutation Pentosyltransferases Phenotype Plant Proteins Plant Roots Polysaccharides Procollagen-Proline Dioxygenase Proline Protein Conformation Protein Processing, Post-Translational Protein Structure, Secondary Arabidopsis thaliana Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana. Fil:Velasquez, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ricardi, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernandez, P.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nadra, A.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00368075_v332_n6036_p1401_Velasquez http://hdl.handle.net/20.500.12110/paper_00368075_v332_n6036_p1401_Velasquez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	arabinogalactan arabinosyltransferase cell membrane protein extensin glycan glycoprotein green fluorescent protein hydroxyproline rich glycoprotein procollagen proline 2 oxoglutarate 4 dioxygenase proline rich protein transferase unclassified drug adsorption dicotyledon enzyme activity hair inhibition polysaccharide protein Arabidopsis arabinosylation article cell growth cell wall chemical analysis chemical reaction glycosylation hydroxylation nonhuman priority journal protein expression protein function root hair Arabidopsis Arabidopsis Proteins Arabinose Carbohydrate Conformation Cell Wall Gene Expression Regulation, Plant Genes, Plant Glycoproteins Glycosylation Glycosyltransferases Hydroxylation Hydroxyproline Models, Biological Mutation Pentosyltransferases Phenotype Plant Proteins Plant Roots Polysaccharides Procollagen-Proline Dioxygenase Proline Protein Conformation Protein Processing, Post-Translational Protein Structure, Secondary Arabidopsis thaliana
spellingShingle	arabinogalactan arabinosyltransferase cell membrane protein extensin glycan glycoprotein green fluorescent protein hydroxyproline rich glycoprotein procollagen proline 2 oxoglutarate 4 dioxygenase proline rich protein transferase unclassified drug adsorption dicotyledon enzyme activity hair inhibition polysaccharide protein Arabidopsis arabinosylation article cell growth cell wall chemical analysis chemical reaction glycosylation hydroxylation nonhuman priority journal protein expression protein function root hair Arabidopsis Arabidopsis Proteins Arabinose Carbohydrate Conformation Cell Wall Gene Expression Regulation, Plant Genes, Plant Glycoproteins Glycosylation Glycosyltransferases Hydroxylation Hydroxyproline Models, Biological Mutation Pentosyltransferases Phenotype Plant Proteins Plant Roots Polysaccharides Procollagen-Proline Dioxygenase Proline Protein Conformation Protein Processing, Post-Translational Protein Structure, Secondary Arabidopsis thaliana Velasquez, Silvia Melina Ricardi, Martiniano Maria Fernández, Paula Virginia Nadra, Alejandro Daniel Ciancia, Marina Iusem, Norberto Daniel Estevez, Jose Manuel O-glycosylated cell wall proteins are essential in root hair growth
topic_facet	arabinogalactan arabinosyltransferase cell membrane protein extensin glycan glycoprotein green fluorescent protein hydroxyproline rich glycoprotein procollagen proline 2 oxoglutarate 4 dioxygenase proline rich protein transferase unclassified drug adsorption dicotyledon enzyme activity hair inhibition polysaccharide protein Arabidopsis arabinosylation article cell growth cell wall chemical analysis chemical reaction glycosylation hydroxylation nonhuman priority journal protein expression protein function root hair Arabidopsis Arabidopsis Proteins Arabinose Carbohydrate Conformation Cell Wall Gene Expression Regulation, Plant Genes, Plant Glycoproteins Glycosylation Glycosyltransferases Hydroxylation Hydroxyproline Models, Biological Mutation Pentosyltransferases Phenotype Plant Proteins Plant Roots Polysaccharides Procollagen-Proline Dioxygenase Proline Protein Conformation Protein Processing, Post-Translational Protein Structure, Secondary Arabidopsis thaliana
description	Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.
author	Velasquez, Silvia Melina Ricardi, Martiniano Maria Fernández, Paula Virginia Nadra, Alejandro Daniel Ciancia, Marina Iusem, Norberto Daniel Estevez, Jose Manuel
author_facet	Velasquez, Silvia Melina Ricardi, Martiniano Maria Fernández, Paula Virginia Nadra, Alejandro Daniel Ciancia, Marina Iusem, Norberto Daniel Estevez, Jose Manuel
author_sort	Velasquez, Silvia Melina
title	O-glycosylated cell wall proteins are essential in root hair growth
title_short	O-glycosylated cell wall proteins are essential in root hair growth
title_full	O-glycosylated cell wall proteins are essential in root hair growth
title_fullStr	O-glycosylated cell wall proteins are essential in root hair growth
title_full_unstemmed	O-glycosylated cell wall proteins are essential in root hair growth
title_sort	o-glycosylated cell wall proteins are essential in root hair growth
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00368075_v332_n6036_p1401_Velasquez http://hdl.handle.net/20.500.12110/paper_00368075_v332_n6036_p1401_Velasquez
work_keys_str_mv	AT velasquezsilviamelina oglycosylatedcellwallproteinsareessentialinroothairgrowth AT ricardimartinianomaria oglycosylatedcellwallproteinsareessentialinroothairgrowth AT fernandezpaulavirginia oglycosylatedcellwallproteinsareessentialinroothairgrowth AT nadraalejandrodaniel oglycosylatedcellwallproteinsareessentialinroothairgrowth AT cianciamarina oglycosylatedcellwallproteinsareessentialinroothairgrowth AT iusemnorbertodaniel oglycosylatedcellwallproteinsareessentialinroothairgrowth AT estevezjosemanuel oglycosylatedcellwallproteinsareessentialinroothairgrowth
_version_	1768544630755819520

O-glycosylated cell wall proteins are essential in root hair growth

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