Differentiation of human embryonic stem cells into cardiomyocytes
Background: The role of stem cells in the treatment of several conditions, especially heart diseases, is under permanent investigation. Human embryonic stem cells have been successfully differentiated in vitro into cardiomyocytes. Methods of cell culture and cardiomyocyte differentiation are well es...
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2009
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paper:paper_00347000_v77_n6_p458_Scassa2023-06-08T15:00:58Z Differentiation of human embryonic stem cells into cardiomyocytes Scassa, María Elida Questa, María Losino, Noelia Ivana Luzzani, Carlos Daniel Guberman, Alejandra Sonia Cardiomyocyte Embryonic stem cells Myogenesis structural protein transcription factor article cell culture cell differentiation cell growth embryonic stem cell heart disease heart muscle cell human human cell immunohistochemistry in vitro study pluripotent stem cell protein expression protein structure reverse transcription polymerase chain reaction signal transduction tissue regeneration Background: The role of stem cells in the treatment of several conditions, especially heart diseases, is under permanent investigation. Human embryonic stem cells have been successfully differentiated in vitro into cardiomyocytes. Methods of cell culture and cardiomyocyte differentiation are well established; signals regulating cardiogenesis have been identified and the cardiomyocytes generated have been used in models of myocardial regeneration. However, several questions still remain and are currently under active investigation. Objective: To develop a culture system that is suitable for the induction of embryonic stem cells to cardiomyocyte differentiation. Material and Methods: Four human embryonic stem cell lines were used. The cells were cultured and differentiation was induced using methods previously described. The presence of cells in an undifferentiated state and cardiomyocyte differentiation was detected by immunohistochemical studies (fluorescent staining) and RT-PCR. Results: The methodology used allowed stem cells growth in the culture, and maintained them in an undifferentiated state. Cardiomyocyte differentiation was achieved in the four cell lines used, yet with uneven efficacy. This was confirmed by the expression of myocardial transcription factors and heart structural proteins. Conclusions: Our system allowed human embryonic stem cell growth and differentiation in the culture. These preliminary results encourage us to continue developing our methods with induced pluripotent stem cells. Fil:Scassa, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Questa, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Losino, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00347000_v77_n6_p458_Scassa http://hdl.handle.net/20.500.12110/paper_00347000_v77_n6_p458_Scassa |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cardiomyocyte Embryonic stem cells Myogenesis structural protein transcription factor article cell culture cell differentiation cell growth embryonic stem cell heart disease heart muscle cell human human cell immunohistochemistry in vitro study pluripotent stem cell protein expression protein structure reverse transcription polymerase chain reaction signal transduction tissue regeneration |
| spellingShingle |
Cardiomyocyte Embryonic stem cells Myogenesis structural protein transcription factor article cell culture cell differentiation cell growth embryonic stem cell heart disease heart muscle cell human human cell immunohistochemistry in vitro study pluripotent stem cell protein expression protein structure reverse transcription polymerase chain reaction signal transduction tissue regeneration Scassa, María Elida Questa, María Losino, Noelia Ivana Luzzani, Carlos Daniel Guberman, Alejandra Sonia Differentiation of human embryonic stem cells into cardiomyocytes |
| topic_facet |
Cardiomyocyte Embryonic stem cells Myogenesis structural protein transcription factor article cell culture cell differentiation cell growth embryonic stem cell heart disease heart muscle cell human human cell immunohistochemistry in vitro study pluripotent stem cell protein expression protein structure reverse transcription polymerase chain reaction signal transduction tissue regeneration |
| description |
Background: The role of stem cells in the treatment of several conditions, especially heart diseases, is under permanent investigation. Human embryonic stem cells have been successfully differentiated in vitro into cardiomyocytes. Methods of cell culture and cardiomyocyte differentiation are well established; signals regulating cardiogenesis have been identified and the cardiomyocytes generated have been used in models of myocardial regeneration. However, several questions still remain and are currently under active investigation. Objective: To develop a culture system that is suitable for the induction of embryonic stem cells to cardiomyocyte differentiation. Material and Methods: Four human embryonic stem cell lines were used. The cells were cultured and differentiation was induced using methods previously described. The presence of cells in an undifferentiated state and cardiomyocyte differentiation was detected by immunohistochemical studies (fluorescent staining) and RT-PCR. Results: The methodology used allowed stem cells growth in the culture, and maintained them in an undifferentiated state. Cardiomyocyte differentiation was achieved in the four cell lines used, yet with uneven efficacy. This was confirmed by the expression of myocardial transcription factors and heart structural proteins. Conclusions: Our system allowed human embryonic stem cell growth and differentiation in the culture. These preliminary results encourage us to continue developing our methods with induced pluripotent stem cells. |
| author |
Scassa, María Elida Questa, María Losino, Noelia Ivana Luzzani, Carlos Daniel Guberman, Alejandra Sonia |
| author_facet |
Scassa, María Elida Questa, María Losino, Noelia Ivana Luzzani, Carlos Daniel Guberman, Alejandra Sonia |
| author_sort |
Scassa, María Elida |
| title |
Differentiation of human embryonic stem cells into cardiomyocytes |
| title_short |
Differentiation of human embryonic stem cells into cardiomyocytes |
| title_full |
Differentiation of human embryonic stem cells into cardiomyocytes |
| title_fullStr |
Differentiation of human embryonic stem cells into cardiomyocytes |
| title_full_unstemmed |
Differentiation of human embryonic stem cells into cardiomyocytes |
| title_sort |
differentiation of human embryonic stem cells into cardiomyocytes |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00347000_v77_n6_p458_Scassa http://hdl.handle.net/20.500.12110/paper_00347000_v77_n6_p458_Scassa |
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1768543360742588416 |