Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds

Euterpe edulis Martius, a tropical palm species characterized as highly recalcitrant, accumulated dehydrin proteins in both the endosperm and the embryo of the mature seed, as detected by Western blot analysis and immunogold electron microscopy. Three major bands at molecular masses of approximately...

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Autores principales:	Distéfano, Ana Julia, Lainez, Verónica Renee, Del Vas, Mariana
Publicado:	2007
Materias:	Dehydrin Euterpe edulis Palmito Recalcitrant seed Subcellular immunolocalization Western blot dehydrin proteins, plant unclassified drug vegetable protein Arecaceae article metabolism plant plant seed ultrastructure Western blotting Blotting, Western Plant Proteins Plant Shoots Seeds
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0033183X_v231_n1-2_p1_Panza http://hdl.handle.net/20.500.12110/paper_0033183X_v231_n1-2_p1_Panza
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0033183X_v231_n1-2_p1_Panza
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spelling	paper:paper_0033183X_v231_n1-2_p1_Panza2023-06-08T15:00:23Z Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds Distéfano, Ana Julia Lainez, Verónica Renee Del Vas, Mariana Dehydrin Euterpe edulis Palmito Recalcitrant seed Subcellular immunolocalization Western blot dehydrin proteins, plant unclassified drug vegetable protein Arecaceae article metabolism plant plant seed ultrastructure Western blotting Arecaceae Blotting, Western Plant Proteins Plant Shoots Seeds Euterpe edulis Euterpe edulis Martius, a tropical palm species characterized as highly recalcitrant, accumulated dehydrin proteins in both the endosperm and the embryo of the mature seed, as detected by Western blot analysis and immunogold electron microscopy. Three major bands at molecular masses of approximately 16, 18, and 24 kDa were identified in both samples analysed. Immunogold electron microscopy studies detected the presence of dehydrins in the embryo and endosperm. In both cases, dehydrins were immunolocalized in cytoplasm and chromatin. No labelling associated with either membranes or organelles was detected. It is known that dehydrins are produced as part of the developmental program of orthodox seeds and are also present in some recalcitrant seeds of temperate regions. The constitutive presence of dehydrins in embryos of extremely recalcitrant species of tropical origin has not been previously reported. © 2007 Springer-Verlag. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Láinez, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Vas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0033183X_v231_n1-2_p1_Panza http://hdl.handle.net/20.500.12110/paper_0033183X_v231_n1-2_p1_Panza
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Dehydrin Euterpe edulis Palmito Recalcitrant seed Subcellular immunolocalization Western blot dehydrin proteins, plant unclassified drug vegetable protein Arecaceae article metabolism plant plant seed ultrastructure Western blotting Arecaceae Blotting, Western Plant Proteins Plant Shoots Seeds Euterpe edulis
spellingShingle	Dehydrin Euterpe edulis Palmito Recalcitrant seed Subcellular immunolocalization Western blot dehydrin proteins, plant unclassified drug vegetable protein Arecaceae article metabolism plant plant seed ultrastructure Western blotting Arecaceae Blotting, Western Plant Proteins Plant Shoots Seeds Euterpe edulis Distéfano, Ana Julia Lainez, Verónica Renee Del Vas, Mariana Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds
topic_facet	Dehydrin Euterpe edulis Palmito Recalcitrant seed Subcellular immunolocalization Western blot dehydrin proteins, plant unclassified drug vegetable protein Arecaceae article metabolism plant plant seed ultrastructure Western blotting Arecaceae Blotting, Western Plant Proteins Plant Shoots Seeds Euterpe edulis
description	Euterpe edulis Martius, a tropical palm species characterized as highly recalcitrant, accumulated dehydrin proteins in both the endosperm and the embryo of the mature seed, as detected by Western blot analysis and immunogold electron microscopy. Three major bands at molecular masses of approximately 16, 18, and 24 kDa were identified in both samples analysed. Immunogold electron microscopy studies detected the presence of dehydrins in the embryo and endosperm. In both cases, dehydrins were immunolocalized in cytoplasm and chromatin. No labelling associated with either membranes or organelles was detected. It is known that dehydrins are produced as part of the developmental program of orthodox seeds and are also present in some recalcitrant seeds of temperate regions. The constitutive presence of dehydrins in embryos of extremely recalcitrant species of tropical origin has not been previously reported. © 2007 Springer-Verlag.
author	Distéfano, Ana Julia Lainez, Verónica Renee Del Vas, Mariana
author_facet	Distéfano, Ana Julia Lainez, Verónica Renee Del Vas, Mariana
author_sort	Distéfano, Ana Julia
title	Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds
title_short	Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds
title_full	Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds
title_fullStr	Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds
title_full_unstemmed	Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds
title_sort	detection of dehydrin-like proteins in embryos and endosperm of mature euterpe edulis seeds
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0033183X_v231_n1-2_p1_Panza http://hdl.handle.net/20.500.12110/paper_0033183X_v231_n1-2_p1_Panza
work_keys_str_mv	AT distefanoanajulia detectionofdehydrinlikeproteinsinembryosandendospermofmatureeuterpeedulisseeds AT lainezveronicarenee detectionofdehydrinlikeproteinsinembryosandendospermofmatureeuterpeedulisseeds AT delvasmariana detectionofdehydrinlikeproteinsinembryosandendospermofmatureeuterpeedulisseeds
_version_	1768543268044275712

Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds

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