Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia

Four macrocyclic trichothecenes, roridin A, roridin E, verrucarin A and verrucarin J, produced by the hypocrealean epibiont of Baccharis coridifolia, were evaluated for their inhibitory activity against the arenavirus Junin (JUNV), the etiological agent of Argentine hemorrhagic fever. The trichothec...

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Autores principales: García, Cybele Carina, Rosso, María Luciana, Bertoni, María Delia Luisa, Maier, Marta Silvia, Damonte, Elsa Beatriz
Publicado: 2002
Materias:
Antiviral activity
Arenaviruses
Asteraceae
Baccharis coridifolia
Junin virus
Trichothecenes
antivirus agent
macrocyclic compound
roridin A
roridin e
sesquiterpenoid
unclassified drug
verrucarin A
verrucarin j
animal cell
antiviral activity
Arenavirus
article
baccharis coridifolia
cell viability
concentration response
controlled study
dose response
drug screening
fungus
hemorrhagic fever
IC 50
nonhuman
virion
virus adsorption
virus inhibition
Animals
Antiviral Agents
Cercopithecus aethiops
Dose-Response Relationship, Drug
Plant Extracts
Vero Cells
Animalia
Argentina (fish)
Baccharis
Fungi
Hypocreales
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320943_v68_n3_p209_Garcia
http://hdl.handle.net/20.500.12110/paper_00320943_v68_n3_p209_Garcia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00320943_v68_n3_p209_Garcia
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spelling paper:paper_00320943_v68_n3_p209_Garcia2023-06-08T15:00:06Z Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia García, Cybele Carina Rosso, María Luciana Bertoni, María Delia Luisa Maier, Marta Silvia Damonte, Elsa Beatriz Antiviral activity Arenaviruses Asteraceae Baccharis coridifolia Junin virus Trichothecenes antivirus agent macrocyclic compound roridin A roridin e sesquiterpenoid unclassified drug verrucarin A verrucarin j animal cell antiviral activity Arenavirus article baccharis coridifolia cell viability concentration response controlled study dose response drug screening fungus hemorrhagic fever IC 50 Junin virus nonhuman virion virus adsorption virus inhibition Animals Antiviral Agents Asteraceae Cercopithecus aethiops Dose-Response Relationship, Drug Junin virus Plant Extracts Trichothecenes Vero Cells Animalia Arenavirus Argentina (fish) Asteraceae Baccharis Fungi Hypocreales Junin virus Four macrocyclic trichothecenes, roridin A, roridin E, verrucarin A and verrucarin J, produced by the hypocrealean epibiont of Baccharis coridifolia, were evaluated for their inhibitory activity against the arenavirus Junin (JUNV), the etiological agent of Argentine hemorrhagic fever. The trichothecenes achieved a dose-dependent inhibition of JUNV multiplication at concentrations not affecting cell viability. The 50% inhibitory concentration (IC50) values determined by a virus yield inhibition assay were in the range 1.2-4.9 ng/ml. The most active compound was verrucarin J which reduced JUNV yield more than 2 log units and had a similar effect against the arenavirus Tacaribe. The trichothecenes lacked virucidal effects on JUNV virions. From time of addition and removal experiments, it can be concluded that verrucarin J inhibited a late stage in the replicative cycle of JUNV, after 5 h of adsorption. Fil:García, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rosso, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bertoni, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maier, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320943_v68_n3_p209_Garcia http://hdl.handle.net/20.500.12110/paper_00320943_v68_n3_p209_Garcia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antiviral activity
Arenaviruses
Asteraceae
Baccharis coridifolia
Junin virus
Trichothecenes
antivirus agent
macrocyclic compound
roridin A
roridin e
sesquiterpenoid
unclassified drug
verrucarin A
verrucarin j
animal cell
antiviral activity
Arenavirus
article
baccharis coridifolia
cell viability
concentration response
controlled study
dose response
drug screening
fungus
hemorrhagic fever
IC 50
Junin virus
nonhuman
virion
virus adsorption
virus inhibition
Animals
Antiviral Agents
Asteraceae
Cercopithecus aethiops
Dose-Response Relationship, Drug
Junin virus
Plant Extracts
Trichothecenes
Vero Cells
Animalia
Arenavirus
Argentina (fish)
Asteraceae
Baccharis
Fungi
Hypocreales
Junin virus
spellingShingle Antiviral activity
Arenaviruses
Asteraceae
Baccharis coridifolia
Junin virus
Trichothecenes
antivirus agent
macrocyclic compound
roridin A
roridin e
sesquiterpenoid
unclassified drug
verrucarin A
verrucarin j
animal cell
antiviral activity
Arenavirus
article
baccharis coridifolia
cell viability
concentration response
controlled study
dose response
drug screening
fungus
hemorrhagic fever
IC 50
Junin virus
nonhuman
virion
virus adsorption
virus inhibition
Animals
Antiviral Agents
Asteraceae
Cercopithecus aethiops
Dose-Response Relationship, Drug
Junin virus
Plant Extracts
Trichothecenes
Vero Cells
Animalia
Arenavirus
Argentina (fish)
Asteraceae
Baccharis
Fungi
Hypocreales
Junin virus
García, Cybele Carina
Rosso, María Luciana
Bertoni, María Delia Luisa
Maier, Marta Silvia
Damonte, Elsa Beatriz
Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia
topic_facet Antiviral activity
Arenaviruses
Asteraceae
Baccharis coridifolia
Junin virus
Trichothecenes
antivirus agent
macrocyclic compound
roridin A
roridin e
sesquiterpenoid
unclassified drug
verrucarin A
verrucarin j
animal cell
antiviral activity
Arenavirus
article
baccharis coridifolia
cell viability
concentration response
controlled study
dose response
drug screening
fungus
hemorrhagic fever
IC 50
Junin virus
nonhuman
virion
virus adsorption
virus inhibition
Animals
Antiviral Agents
Asteraceae
Cercopithecus aethiops
Dose-Response Relationship, Drug
Junin virus
Plant Extracts
Trichothecenes
Vero Cells
Animalia
Arenavirus
Argentina (fish)
Asteraceae
Baccharis
Fungi
Hypocreales
Junin virus
description Four macrocyclic trichothecenes, roridin A, roridin E, verrucarin A and verrucarin J, produced by the hypocrealean epibiont of Baccharis coridifolia, were evaluated for their inhibitory activity against the arenavirus Junin (JUNV), the etiological agent of Argentine hemorrhagic fever. The trichothecenes achieved a dose-dependent inhibition of JUNV multiplication at concentrations not affecting cell viability. The 50% inhibitory concentration (IC50) values determined by a virus yield inhibition assay were in the range 1.2-4.9 ng/ml. The most active compound was verrucarin J which reduced JUNV yield more than 2 log units and had a similar effect against the arenavirus Tacaribe. The trichothecenes lacked virucidal effects on JUNV virions. From time of addition and removal experiments, it can be concluded that verrucarin J inhibited a late stage in the replicative cycle of JUNV, after 5 h of adsorption.
author García, Cybele Carina
Rosso, María Luciana
Bertoni, María Delia Luisa
Maier, Marta Silvia
Damonte, Elsa Beatriz
author_facet García, Cybele Carina
Rosso, María Luciana
Bertoni, María Delia Luisa
Maier, Marta Silvia
Damonte, Elsa Beatriz
author_sort García, Cybele Carina
title Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia
title_short Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia
title_full Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia
title_fullStr Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia
title_full_unstemmed Evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia
title_sort evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of baccharis coridifolia
publishDate 2002
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320943_v68_n3_p209_Garcia
http://hdl.handle.net/20.500.12110/paper_00320943_v68_n3_p209_Garcia
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