Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves
Tuber formation in potato (Solanum tuberosum L.) is regulated by hormonal and environmental signals that are thought to be integrated in the leaves. The molecular mechanisms that mediate the responses to tuberization-related signals in leaves remain largely unknown. In this study we analyzed the rol...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v232_n1_p37_Pais http://hdl.handle.net/20.500.12110/paper_00320935_v232_n1_p37_Pais |
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paper:paper_00320935_v232_n1_p37_Pais2023-06-08T15:00:03Z Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves Pais, Silvia Marina Gibberellic acid Patatin Pin2 PP2A Sucrose Tuberization phosphoprotein phosphatase 2 primer DNA amino acid sequence article chemistry enzyme active site enzymology metabolism molecular genetics nucleotide sequence plant leaf potato reverse transcription polymerase chain reaction sequence homology signal transduction Western blotting Amino Acid Sequence Base Sequence Blotting, Western Catalytic Domain DNA Primers Molecular Sequence Data Plant Leaves Protein Phosphatase 2 Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Solanum tuberosum Tuber formation in potato (Solanum tuberosum L.) is regulated by hormonal and environmental signals that are thought to be integrated in the leaves. The molecular mechanisms that mediate the responses to tuberization-related signals in leaves remain largely unknown. In this study we analyzed the roles of protein phosphatase type 2A catalytic subunits (PP2Ac) in the leaf responses to conditions that affect tuberization. The responses were monitored by analyzing the expression of the "tuber-specific" genes Patatin and Pin2, which are induced in tubers and leaves during tuber induction. Experiments using PP2A inhibitors, together with PP2Ac expression profiles under conditions that affect tuberization indicate that high sucrose/nitrogen ratio, which promotes tuber formation, increases the transcript levels of Patatin and Pin2, by increasing the activity of PP2As without affecting PP2Ac mRNA or protein levels. Gibberellic acid (GA), a negative regulator of tuberization, down-regulates the transcription of catalytic subunits of PP2As from the subfamily I and decreases their enzyme levels. In addition, GA inhibits the expression of Patatin and Pin2 possibly by a PP2A-independent mechanism. PP2Ac down-regulation by GA may inhibit tuberization signaling downstream of the inductive effects of high sucrose/nitrogen ratio. These results are consistent with the hypothesis that PP2As of the subfamily I may positively modulate the signaling pathways that lead to the transcriptional activation of "tuber-specific" genes in leaves, and act as molecular switches regulated by both positive and negative modulators of tuberization. © 2010 Springer-Verlag. Fil:País, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v232_n1_p37_Pais http://hdl.handle.net/20.500.12110/paper_00320935_v232_n1_p37_Pais |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Gibberellic acid Patatin Pin2 PP2A Sucrose Tuberization phosphoprotein phosphatase 2 primer DNA amino acid sequence article chemistry enzyme active site enzymology metabolism molecular genetics nucleotide sequence plant leaf potato reverse transcription polymerase chain reaction sequence homology signal transduction Western blotting Amino Acid Sequence Base Sequence Blotting, Western Catalytic Domain DNA Primers Molecular Sequence Data Plant Leaves Protein Phosphatase 2 Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Solanum tuberosum |
| spellingShingle |
Gibberellic acid Patatin Pin2 PP2A Sucrose Tuberization phosphoprotein phosphatase 2 primer DNA amino acid sequence article chemistry enzyme active site enzymology metabolism molecular genetics nucleotide sequence plant leaf potato reverse transcription polymerase chain reaction sequence homology signal transduction Western blotting Amino Acid Sequence Base Sequence Blotting, Western Catalytic Domain DNA Primers Molecular Sequence Data Plant Leaves Protein Phosphatase 2 Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Solanum tuberosum Pais, Silvia Marina Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves |
| topic_facet |
Gibberellic acid Patatin Pin2 PP2A Sucrose Tuberization phosphoprotein phosphatase 2 primer DNA amino acid sequence article chemistry enzyme active site enzymology metabolism molecular genetics nucleotide sequence plant leaf potato reverse transcription polymerase chain reaction sequence homology signal transduction Western blotting Amino Acid Sequence Base Sequence Blotting, Western Catalytic Domain DNA Primers Molecular Sequence Data Plant Leaves Protein Phosphatase 2 Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Solanum tuberosum |
| description |
Tuber formation in potato (Solanum tuberosum L.) is regulated by hormonal and environmental signals that are thought to be integrated in the leaves. The molecular mechanisms that mediate the responses to tuberization-related signals in leaves remain largely unknown. In this study we analyzed the roles of protein phosphatase type 2A catalytic subunits (PP2Ac) in the leaf responses to conditions that affect tuberization. The responses were monitored by analyzing the expression of the "tuber-specific" genes Patatin and Pin2, which are induced in tubers and leaves during tuber induction. Experiments using PP2A inhibitors, together with PP2Ac expression profiles under conditions that affect tuberization indicate that high sucrose/nitrogen ratio, which promotes tuber formation, increases the transcript levels of Patatin and Pin2, by increasing the activity of PP2As without affecting PP2Ac mRNA or protein levels. Gibberellic acid (GA), a negative regulator of tuberization, down-regulates the transcription of catalytic subunits of PP2As from the subfamily I and decreases their enzyme levels. In addition, GA inhibits the expression of Patatin and Pin2 possibly by a PP2A-independent mechanism. PP2Ac down-regulation by GA may inhibit tuberization signaling downstream of the inductive effects of high sucrose/nitrogen ratio. These results are consistent with the hypothesis that PP2As of the subfamily I may positively modulate the signaling pathways that lead to the transcriptional activation of "tuber-specific" genes in leaves, and act as molecular switches regulated by both positive and negative modulators of tuberization. © 2010 Springer-Verlag. |
| author |
Pais, Silvia Marina |
| author_facet |
Pais, Silvia Marina |
| author_sort |
Pais, Silvia Marina |
| title |
Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves |
| title_short |
Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves |
| title_full |
Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves |
| title_fullStr |
Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves |
| title_full_unstemmed |
Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves |
| title_sort |
protein phosphatases type 2a mediate tuberization signaling in solanum tuberosum l. leaves |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v232_n1_p37_Pais http://hdl.handle.net/20.500.12110/paper_00320935_v232_n1_p37_Pais |
| work_keys_str_mv |
AT paissilviamarina proteinphosphatasestype2amediatetuberizationsignalinginsolanumtuberosumlleaves |
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1768545454338867200 |