Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition

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Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overex...

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Autores principales:	Almasia, Natalia Inés, Fernández, Paula Virginia, Hopp, Horacio Esteban, Estevez, Jose Manuel, Carrari, Fernando O., Vazquez Rovere, Cecilia
Publicado:	2012
Materias:	green fluorescent protein SN1 protein, Solanum tuberosum vegetable protein article cell division cell membrane cell wall chemistry cytology gene expression regulation gene silencing genetics infrared spectroscopy mass fragmentography metabolism molecular genetics nucleotide sequence physiology plant epidermis plant leaf potato Solanaceae transgenic plant Cell Division Cell Membrane Cell Wall Gas Chromatography-Mass Spectrometry Gene Expression Regulation, Plant Gene Silencing Green Fluorescent Proteins Molecular Sequence Data Plant Epidermis Plant Leaves Plant Proteins Plants, Genetically Modified Solanum tuberosum Spectroscopy, Fourier Transform Infrared Arabidopsis Nicotiana benthamiana
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v158_n1_p252_Nahirnak http://hdl.handle.net/20.500.12110/paper_00320889_v158_n1_p252_Nahirnak
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00320889_v158_n1_p252_Nahirnak2023-06-08T15:00:00Z Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition Almasia, Natalia Inés Fernández, Paula Virginia Hopp, Horacio Esteban Estevez, Jose Manuel Carrari, Fernando O. Vazquez Rovere, Cecilia green fluorescent protein SN1 protein, Solanum tuberosum vegetable protein article cell division cell membrane cell wall chemistry cytology gene expression regulation gene silencing genetics infrared spectroscopy mass fragmentography metabolism molecular genetics nucleotide sequence physiology plant epidermis plant leaf potato Solanaceae transgenic plant Cell Division Cell Membrane Cell Wall Gas Chromatography-Mass Spectrometry Gene Expression Regulation, Plant Gene Silencing Green Fluorescent Proteins Molecular Sequence Data Plant Epidermis Plant Leaves Plant Proteins Plants, Genetically Modified Solanaceae Solanum tuberosum Spectroscopy, Fourier Transform Infrared Arabidopsis Nicotiana benthamiana Solanum tuberosum Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was accompanied by an overall reduction in leaf size and severe alterations of leaf shape. Analysis of the adaxial epidermis of mature leaves revealed that silenced lines had 70% to 90% increases in mean cell size with respect to wild-type leaves. Consequently, the number of epidermal cells was significantly reduced in these lines. Confocal microscopy analysis after agroinfiltration of Nicotiana benthamiana leaves showed that SN1-green fluorescent protein fusion protein was localized in plasma membrane, and bimolecular fluorescence complementation assays revealed that SN1 self-interacted in vivo. We further focused our study on leaf metabolism by applying a combination of gas chromatography coupled to mass spectrometry, Fourier transform infrared spectroscopy, and spectrophotometric techniques. These targeted analyses allowed a detailed examination of the changes occurring in 46 intermediate compounds from primary metabolic pathways and in seven cell wall constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense. © 2011 American Society of Plant Biologists. All Rights Reserved. Fil:Almasia, N.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernandez, P.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carrari, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez-Rovere, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v158_n1_p252_Nahirnak http://hdl.handle.net/20.500.12110/paper_00320889_v158_n1_p252_Nahirnak
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	green fluorescent protein SN1 protein, Solanum tuberosum vegetable protein article cell division cell membrane cell wall chemistry cytology gene expression regulation gene silencing genetics infrared spectroscopy mass fragmentography metabolism molecular genetics nucleotide sequence physiology plant epidermis plant leaf potato Solanaceae transgenic plant Cell Division Cell Membrane Cell Wall Gas Chromatography-Mass Spectrometry Gene Expression Regulation, Plant Gene Silencing Green Fluorescent Proteins Molecular Sequence Data Plant Epidermis Plant Leaves Plant Proteins Plants, Genetically Modified Solanaceae Solanum tuberosum Spectroscopy, Fourier Transform Infrared Arabidopsis Nicotiana benthamiana Solanum tuberosum
spellingShingle	green fluorescent protein SN1 protein, Solanum tuberosum vegetable protein article cell division cell membrane cell wall chemistry cytology gene expression regulation gene silencing genetics infrared spectroscopy mass fragmentography metabolism molecular genetics nucleotide sequence physiology plant epidermis plant leaf potato Solanaceae transgenic plant Cell Division Cell Membrane Cell Wall Gas Chromatography-Mass Spectrometry Gene Expression Regulation, Plant Gene Silencing Green Fluorescent Proteins Molecular Sequence Data Plant Epidermis Plant Leaves Plant Proteins Plants, Genetically Modified Solanaceae Solanum tuberosum Spectroscopy, Fourier Transform Infrared Arabidopsis Nicotiana benthamiana Solanum tuberosum Almasia, Natalia Inés Fernández, Paula Virginia Hopp, Horacio Esteban Estevez, Jose Manuel Carrari, Fernando O. Vazquez Rovere, Cecilia Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition
topic_facet	green fluorescent protein SN1 protein, Solanum tuberosum vegetable protein article cell division cell membrane cell wall chemistry cytology gene expression regulation gene silencing genetics infrared spectroscopy mass fragmentography metabolism molecular genetics nucleotide sequence physiology plant epidermis plant leaf potato Solanaceae transgenic plant Cell Division Cell Membrane Cell Wall Gas Chromatography-Mass Spectrometry Gene Expression Regulation, Plant Gene Silencing Green Fluorescent Proteins Molecular Sequence Data Plant Epidermis Plant Leaves Plant Proteins Plants, Genetically Modified Solanaceae Solanum tuberosum Spectroscopy, Fourier Transform Infrared Arabidopsis Nicotiana benthamiana Solanum tuberosum
description	Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was accompanied by an overall reduction in leaf size and severe alterations of leaf shape. Analysis of the adaxial epidermis of mature leaves revealed that silenced lines had 70% to 90% increases in mean cell size with respect to wild-type leaves. Consequently, the number of epidermal cells was significantly reduced in these lines. Confocal microscopy analysis after agroinfiltration of Nicotiana benthamiana leaves showed that SN1-green fluorescent protein fusion protein was localized in plasma membrane, and bimolecular fluorescence complementation assays revealed that SN1 self-interacted in vivo. We further focused our study on leaf metabolism by applying a combination of gas chromatography coupled to mass spectrometry, Fourier transform infrared spectroscopy, and spectrophotometric techniques. These targeted analyses allowed a detailed examination of the changes occurring in 46 intermediate compounds from primary metabolic pathways and in seven cell wall constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense. © 2011 American Society of Plant Biologists. All Rights Reserved.
author	Almasia, Natalia Inés Fernández, Paula Virginia Hopp, Horacio Esteban Estevez, Jose Manuel Carrari, Fernando O. Vazquez Rovere, Cecilia
author_facet	Almasia, Natalia Inés Fernández, Paula Virginia Hopp, Horacio Esteban Estevez, Jose Manuel Carrari, Fernando O. Vazquez Rovere, Cecilia
author_sort	Almasia, Natalia Inés
title	Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition
title_short	Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition
title_full	Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition
title_fullStr	Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition
title_full_unstemmed	Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition
title_sort	potato snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v158_n1_p252_Nahirnak http://hdl.handle.net/20.500.12110/paper_00320889_v158_n1_p252_Nahirnak
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_version_	1768541598216355840

Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition

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