Molecular characterization of a putative sucrose:Fructan 6-fructosyltransferase (6-SFT) of the cold-resistant patagonian grass bromus pictus associated with fructan accumulation under low temperatures
Fructans are fructose polymers synthesized from sucrose in the plant vacuole. They represent short- and long-term carbohydrate reserves and have been associated with abiotic stress tolerance in graminean species. We report the isolation and characterization of a putative sucrose: fructan 6-fructosyl...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320781_v50_n3_p489_DelViso http://hdl.handle.net/20.500.12110/paper_00320781_v50_n3_p489_DelViso |
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paper:paper_00320781_v50_n3_p489_DelViso2023-06-08T14:59:54Z Molecular characterization of a putative sucrose:Fructan 6-fructosyltransferase (6-SFT) of the cold-resistant patagonian grass bromus pictus associated with fructan accumulation under low temperatures del Viso, Florencia Puebla, Andrea Fabiana Fusari, Corina Mariana Casabuono, Adriana Cristina Couto, Alicia Susana Pontis Videla, Horacio G. Hopp, Horacio Esteban Heinz, Ruth Amelia 6-SFT Abiotic stress Bromus pictus Fructan Low temperatures Patagonia Bromus Bromus pictus Graminea complementary DNA fructan glycosyltransferase plant RNA sucrose - fructan 6-fructosyltransferase sucrose fructan 6 fructosyltransferase vegetable protein amino acid sequence article biosynthesis Bromus cold DNA sequence enzymology gene expression regulation genetics metabolism molecular cloning molecular genetics plant gene sequence alignment Amino Acid Sequence Bromus Cloning, Molecular Cold Temperature DNA, Complementary Fructans Gene Expression Regulation, Plant Genes, Plant Hexosyltransferases Molecular Sequence Data Plant Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA Fructans are fructose polymers synthesized from sucrose in the plant vacuole. They represent short- and long-term carbohydrate reserves and have been associated with abiotic stress tolerance in graminean species. We report the isolation and characterization of a putative sucrose: fructan 6-fructosyltransferase (6-SFT) gene from a Patagonian grass species, Bromus pictus, tolerant to drought and cold temperatures. Structural and functional analyses of this gene were performed by Southern and Northern blot. Sugar content, quality and fructosyltransferase activity were studied using HPAEC-PAD (high-pH anion-exchange chromatography with pulsed amperometric detection),enzymatic and colorimetric assays. The putative 6-SFT gene had all the conserved motifs of fructosyl-transferase and showed 90% identity at the amino acid level with other 6-SFTs from winter cereals. Expression studies, and determination of sugar content and fructosyl-transferase activity were performed on fi ve sections of the leaf. Bp6- SFT wa s expressed predominantly in leaf bases, where fructosyltransferase activity and fructan content are higher. Bp6-SFT expression and accumulation of fructans showed different patterns in the evaluated leaf sections during a 7 d time course experiment under chilling treatment. The transcriptional pattern suggests that the B. pictus 6-SFT gene is highly expressed in basal leaf sections even under control temperate conditions, in contrast to previous reports in other graminean species. Low temperatures caused an increase in Bp6-SFT expression and fructan accumulation in leaf bases. This is the fi rst study of the isolation and molecular characterization of a fructosyltransferase in a native species from the Patagonian region. Expression in heterologous systems will confi rm the functionality, allowing future developments in generation of functional markers for assisted breeding or biotechnological applications. Fil:Del Viso, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Puebla, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fusari, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casabuono, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pontis, H.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320781_v50_n3_p489_DelViso http://hdl.handle.net/20.500.12110/paper_00320781_v50_n3_p489_DelViso |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
6-SFT Abiotic stress Bromus pictus Fructan Low temperatures Patagonia Bromus Bromus pictus Graminea complementary DNA fructan glycosyltransferase plant RNA sucrose - fructan 6-fructosyltransferase sucrose fructan 6 fructosyltransferase vegetable protein amino acid sequence article biosynthesis Bromus cold DNA sequence enzymology gene expression regulation genetics metabolism molecular cloning molecular genetics plant gene sequence alignment Amino Acid Sequence Bromus Cloning, Molecular Cold Temperature DNA, Complementary Fructans Gene Expression Regulation, Plant Genes, Plant Hexosyltransferases Molecular Sequence Data Plant Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA |
| spellingShingle |
6-SFT Abiotic stress Bromus pictus Fructan Low temperatures Patagonia Bromus Bromus pictus Graminea complementary DNA fructan glycosyltransferase plant RNA sucrose - fructan 6-fructosyltransferase sucrose fructan 6 fructosyltransferase vegetable protein amino acid sequence article biosynthesis Bromus cold DNA sequence enzymology gene expression regulation genetics metabolism molecular cloning molecular genetics plant gene sequence alignment Amino Acid Sequence Bromus Cloning, Molecular Cold Temperature DNA, Complementary Fructans Gene Expression Regulation, Plant Genes, Plant Hexosyltransferases Molecular Sequence Data Plant Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA del Viso, Florencia Puebla, Andrea Fabiana Fusari, Corina Mariana Casabuono, Adriana Cristina Couto, Alicia Susana Pontis Videla, Horacio G. Hopp, Horacio Esteban Heinz, Ruth Amelia Molecular characterization of a putative sucrose:Fructan 6-fructosyltransferase (6-SFT) of the cold-resistant patagonian grass bromus pictus associated with fructan accumulation under low temperatures |
| topic_facet |
6-SFT Abiotic stress Bromus pictus Fructan Low temperatures Patagonia Bromus Bromus pictus Graminea complementary DNA fructan glycosyltransferase plant RNA sucrose - fructan 6-fructosyltransferase sucrose fructan 6 fructosyltransferase vegetable protein amino acid sequence article biosynthesis Bromus cold DNA sequence enzymology gene expression regulation genetics metabolism molecular cloning molecular genetics plant gene sequence alignment Amino Acid Sequence Bromus Cloning, Molecular Cold Temperature DNA, Complementary Fructans Gene Expression Regulation, Plant Genes, Plant Hexosyltransferases Molecular Sequence Data Plant Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA |
| description |
Fructans are fructose polymers synthesized from sucrose in the plant vacuole. They represent short- and long-term carbohydrate reserves and have been associated with abiotic stress tolerance in graminean species. We report the isolation and characterization of a putative sucrose: fructan 6-fructosyltransferase (6-SFT) gene from a Patagonian grass species, Bromus pictus, tolerant to drought and cold temperatures. Structural and functional analyses of this gene were performed by Southern and Northern blot. Sugar content, quality and fructosyltransferase activity were studied using HPAEC-PAD (high-pH anion-exchange chromatography with pulsed amperometric detection),enzymatic and colorimetric assays. The putative 6-SFT gene had all the conserved motifs of fructosyl-transferase and showed 90% identity at the amino acid level with other 6-SFTs from winter cereals. Expression studies, and determination of sugar content and fructosyl-transferase activity were performed on fi ve sections of the leaf. Bp6- SFT wa s expressed predominantly in leaf bases, where fructosyltransferase activity and fructan content are higher. Bp6-SFT expression and accumulation of fructans showed different patterns in the evaluated leaf sections during a 7 d time course experiment under chilling treatment. The transcriptional pattern suggests that the B. pictus 6-SFT gene is highly expressed in basal leaf sections even under control temperate conditions, in contrast to previous reports in other graminean species. Low temperatures caused an increase in Bp6-SFT expression and fructan accumulation in leaf bases. This is the fi rst study of the isolation and molecular characterization of a fructosyltransferase in a native species from the Patagonian region. Expression in heterologous systems will confi rm the functionality, allowing future developments in generation of functional markers for assisted breeding or biotechnological applications. |
| author |
del Viso, Florencia Puebla, Andrea Fabiana Fusari, Corina Mariana Casabuono, Adriana Cristina Couto, Alicia Susana Pontis Videla, Horacio G. Hopp, Horacio Esteban Heinz, Ruth Amelia |
| author_facet |
del Viso, Florencia Puebla, Andrea Fabiana Fusari, Corina Mariana Casabuono, Adriana Cristina Couto, Alicia Susana Pontis Videla, Horacio G. Hopp, Horacio Esteban Heinz, Ruth Amelia |
| author_sort |
del Viso, Florencia |
| title |
Molecular characterization of a putative sucrose:Fructan 6-fructosyltransferase (6-SFT) of the cold-resistant patagonian grass bromus pictus associated with fructan accumulation under low temperatures |
| title_short |
Molecular characterization of a putative sucrose:Fructan 6-fructosyltransferase (6-SFT) of the cold-resistant patagonian grass bromus pictus associated with fructan accumulation under low temperatures |
| title_full |
Molecular characterization of a putative sucrose:Fructan 6-fructosyltransferase (6-SFT) of the cold-resistant patagonian grass bromus pictus associated with fructan accumulation under low temperatures |
| title_fullStr |
Molecular characterization of a putative sucrose:Fructan 6-fructosyltransferase (6-SFT) of the cold-resistant patagonian grass bromus pictus associated with fructan accumulation under low temperatures |
| title_full_unstemmed |
Molecular characterization of a putative sucrose:Fructan 6-fructosyltransferase (6-SFT) of the cold-resistant patagonian grass bromus pictus associated with fructan accumulation under low temperatures |
| title_sort |
molecular characterization of a putative sucrose:fructan 6-fructosyltransferase (6-sft) of the cold-resistant patagonian grass bromus pictus associated with fructan accumulation under low temperatures |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320781_v50_n3_p489_DelViso http://hdl.handle.net/20.500.12110/paper_00320781_v50_n3_p489_DelViso |
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