Organogenesis induction in rice callus by cyanobacterial extracellular products
Cyanobacteria are prokaryotic photosynthetic micro-organisms that produce a wide array of bioactive substances, including plant growth regulators. Many substances have been added to plant tissue culture media in order to promote plant regeneration. We studied the effect of extracellular products fro...
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2002
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paper:paper_00319449_v52_n4_p263_Zaccaro2023-06-08T14:59:46Z Organogenesis induction in rice callus by cyanobacterial extracellular products Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina Storni de Cano, Mónica M. Zulpa de Caire, Gloria A,b- total chlorophyll Microchaete tenera Plant regeneration Rice callus histology Acetic acid Agriculture Biomass Biomaterials Chlorophyll Histology Photosynthesis Plants (botany) Tissue culture Cyanobacteria Bacteria Acetic Acid Bacteria Chlorophylls Histology Photosynthesis Plants Tissue Culture Cyanobacteria Microchaete tenera Oryza sativa Prokaryota Cyanobacteria are prokaryotic photosynthetic micro-organisms that produce a wide array of bioactive substances, including plant growth regulators. Many substances have been added to plant tissue culture media in order to promote plant regeneration. We studied the effect of extracellular products from Microchaete tenera Thuret ex. Born. et Flah. strain 14b on regeneration, pigments content and histology of rice callus. Calli obtained from mature embryos of Oryza sativa L. cv. Yerua PA in Murashige & Skoog's medium + 3mg/l 2,4-dichlorophenoxyacetic acid were cultivated in regeneration media, Murashige & Skoog's medium + extracellular products or Murashige & Skoog's medium + 0.5mg/l 6-benzyladenine. Histological studies confirmed the presence of shoots and roots in calli treated with cyanobacterial extracellular products and only roots with 6-benzyladenine. Chlorophyll a, b and total, and carotenoids contents in extracellular products-treated calli were: 5.01 ± 0.41, 1.81 ± 0.20, 7.87 ± 0.65 and 1.97 ± 0.21 (μg/g fresh weight), respectively and chlorophyll a/b and carotenoids/total chlorophyll ratio, 2.77 ± 0.19 and 0.25 ± 0.02 respectively. With 6-benzyladenine the values were 14.21 ± 1.40, 17.21 ± 1.68, 31.33 ± 0.28 and 3.92 ± 0.28 and 0.82 ± 0.10 and 0.12 ± 0.01, respectively. Biochemical studies indicate that chlorophyll content is not positively correlated with organogenesis. Organogenesis induction is consistent with the probable presence of plant growth regulators in cyanobacterial extracellular products which could result in beneficial effects when applied to agricultural practice. Fil:Zaccaro, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stella, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Storni De Cano, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zulpa De Caire, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319449_v52_n4_p263_Zaccaro http://hdl.handle.net/20.500.12110/paper_00319449_v52_n4_p263_Zaccaro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
A,b- total chlorophyll Microchaete tenera Plant regeneration Rice callus histology Acetic acid Agriculture Biomass Biomaterials Chlorophyll Histology Photosynthesis Plants (botany) Tissue culture Cyanobacteria Bacteria Acetic Acid Bacteria Chlorophylls Histology Photosynthesis Plants Tissue Culture Cyanobacteria Microchaete tenera Oryza sativa Prokaryota |
| spellingShingle |
A,b- total chlorophyll Microchaete tenera Plant regeneration Rice callus histology Acetic acid Agriculture Biomass Biomaterials Chlorophyll Histology Photosynthesis Plants (botany) Tissue culture Cyanobacteria Bacteria Acetic Acid Bacteria Chlorophylls Histology Photosynthesis Plants Tissue Culture Cyanobacteria Microchaete tenera Oryza sativa Prokaryota Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina Storni de Cano, Mónica M. Zulpa de Caire, Gloria Organogenesis induction in rice callus by cyanobacterial extracellular products |
| topic_facet |
A,b- total chlorophyll Microchaete tenera Plant regeneration Rice callus histology Acetic acid Agriculture Biomass Biomaterials Chlorophyll Histology Photosynthesis Plants (botany) Tissue culture Cyanobacteria Bacteria Acetic Acid Bacteria Chlorophylls Histology Photosynthesis Plants Tissue Culture Cyanobacteria Microchaete tenera Oryza sativa Prokaryota |
| description |
Cyanobacteria are prokaryotic photosynthetic micro-organisms that produce a wide array of bioactive substances, including plant growth regulators. Many substances have been added to plant tissue culture media in order to promote plant regeneration. We studied the effect of extracellular products from Microchaete tenera Thuret ex. Born. et Flah. strain 14b on regeneration, pigments content and histology of rice callus. Calli obtained from mature embryos of Oryza sativa L. cv. Yerua PA in Murashige & Skoog's medium + 3mg/l 2,4-dichlorophenoxyacetic acid were cultivated in regeneration media, Murashige & Skoog's medium + extracellular products or Murashige & Skoog's medium + 0.5mg/l 6-benzyladenine. Histological studies confirmed the presence of shoots and roots in calli treated with cyanobacterial extracellular products and only roots with 6-benzyladenine. Chlorophyll a, b and total, and carotenoids contents in extracellular products-treated calli were: 5.01 ± 0.41, 1.81 ± 0.20, 7.87 ± 0.65 and 1.97 ± 0.21 (μg/g fresh weight), respectively and chlorophyll a/b and carotenoids/total chlorophyll ratio, 2.77 ± 0.19 and 0.25 ± 0.02 respectively. With 6-benzyladenine the values were 14.21 ± 1.40, 17.21 ± 1.68, 31.33 ± 0.28 and 3.92 ± 0.28 and 0.82 ± 0.10 and 0.12 ± 0.01, respectively. Biochemical studies indicate that chlorophyll content is not positively correlated with organogenesis. Organogenesis induction is consistent with the probable presence of plant growth regulators in cyanobacterial extracellular products which could result in beneficial effects when applied to agricultural practice. |
| author |
Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina Storni de Cano, Mónica M. Zulpa de Caire, Gloria |
| author_facet |
Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina Storni de Cano, Mónica M. Zulpa de Caire, Gloria |
| author_sort |
Zaccaro, María Cristina |
| title |
Organogenesis induction in rice callus by cyanobacterial extracellular products |
| title_short |
Organogenesis induction in rice callus by cyanobacterial extracellular products |
| title_full |
Organogenesis induction in rice callus by cyanobacterial extracellular products |
| title_fullStr |
Organogenesis induction in rice callus by cyanobacterial extracellular products |
| title_full_unstemmed |
Organogenesis induction in rice callus by cyanobacterial extracellular products |
| title_sort |
organogenesis induction in rice callus by cyanobacterial extracellular products |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319449_v52_n4_p263_Zaccaro http://hdl.handle.net/20.500.12110/paper_00319449_v52_n4_p263_Zaccaro |
| work_keys_str_mv |
AT zaccaromariacristina organogenesisinductioninricecallusbycyanobacterialextracellularproducts AT stelladerosellinianamariacristina organogenesisinductioninricecallusbycyanobacterialextracellularproducts AT stornidecanomonicam organogenesisinductioninricecallusbycyanobacterialextracellularproducts AT zulpadecairegloria organogenesisinductioninricecallusbycyanobacterialextracellularproducts |
| _version_ |
1768543074674278400 |