Sulfated mannans from the red seaweed Nemalion helminthoides of the South Atlantic
Nemalion helminthoides, collected in the Argentine South Atlantic coast, was extracted with hot water and the crude product fractionated using cetrimide. The complexed material was subjected to fractional solubilization in solutions of increasing sodium chloride concentration and seven fractions wer...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319422_v70_n8_p1062_PerezRecalde http://hdl.handle.net/20.500.12110/paper_00319422_v70_n8_p1062_PerezRecalde |
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paper:paper_00319422_v70_n8_p1062_PerezRecalde2023-06-08T14:59:40Z Sulfated mannans from the red seaweed Nemalion helminthoides of the South Atlantic Pérez Recalde, María Mercedes Noseda, Miguel D. Pujol, Carlos Alberto Carlucci, María Josefina Matulewicz, María Cristina Antiherpetic activity Nemaliales Nemalion helminthoides Red seaweed Sulfated (1 → 3)-linked α-d-mannans Sulfated mannans mannan sulfate animal article Cercopithecus chemical structure chemistry drug effect Herpes simplex virus isolation and purification seaweed Vero cell Animals Cercopithecus aethiops Mannans Molecular Structure Seaweed Simplexvirus Sulfuric Acid Esters Vero Cells Nemaliales Nemalion helminthoides Nemalion helminthoides, collected in the Argentine South Atlantic coast, was extracted with hot water and the crude product fractionated using cetrimide. The complexed material was subjected to fractional solubilization in solutions of increasing sodium chloride concentration and seven fractions were separated and analyzed. Structural analysis of the main fractions, those soluble in 3.0 and 4.0 M NaCl (yields 21.0% and 13.8%, respectively) and those insoluble in 4.0 M NaCl (yield 20.0%), indicated that this seaweed biosynthesizes (1 → 3)-linked α-d-mannans that are sulfated at positions 4 and 6. Three mannan fractions comprising considerable amounts of xylose were also isolated in very low total yield (2.0%). The fractions that were soluble in 3.0 and 4.0 M NaCl showed low antiherpetic activity whereas this activity was considerable for the fraction solubilized in 2.0 M NaCl (yield 0.5%) which contained single stubs of β-d-xylose. A xylan, soluble in cetrimide solution, containing (1 → 3, 1 → 4)-linked β-d-xylose residues, was also isolated in minor amount. © 2009 Elsevier Ltd. All rights reserved. Fil:Pérez Recalde, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Noseda, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carlucci, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319422_v70_n8_p1062_PerezRecalde http://hdl.handle.net/20.500.12110/paper_00319422_v70_n8_p1062_PerezRecalde |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiherpetic activity Nemaliales Nemalion helminthoides Red seaweed Sulfated (1 → 3)-linked α-d-mannans Sulfated mannans mannan sulfate animal article Cercopithecus chemical structure chemistry drug effect Herpes simplex virus isolation and purification seaweed Vero cell Animals Cercopithecus aethiops Mannans Molecular Structure Seaweed Simplexvirus Sulfuric Acid Esters Vero Cells Nemaliales Nemalion helminthoides |
| spellingShingle |
Antiherpetic activity Nemaliales Nemalion helminthoides Red seaweed Sulfated (1 → 3)-linked α-d-mannans Sulfated mannans mannan sulfate animal article Cercopithecus chemical structure chemistry drug effect Herpes simplex virus isolation and purification seaweed Vero cell Animals Cercopithecus aethiops Mannans Molecular Structure Seaweed Simplexvirus Sulfuric Acid Esters Vero Cells Nemaliales Nemalion helminthoides Pérez Recalde, María Mercedes Noseda, Miguel D. Pujol, Carlos Alberto Carlucci, María Josefina Matulewicz, María Cristina Sulfated mannans from the red seaweed Nemalion helminthoides of the South Atlantic |
| topic_facet |
Antiherpetic activity Nemaliales Nemalion helminthoides Red seaweed Sulfated (1 → 3)-linked α-d-mannans Sulfated mannans mannan sulfate animal article Cercopithecus chemical structure chemistry drug effect Herpes simplex virus isolation and purification seaweed Vero cell Animals Cercopithecus aethiops Mannans Molecular Structure Seaweed Simplexvirus Sulfuric Acid Esters Vero Cells Nemaliales Nemalion helminthoides |
| description |
Nemalion helminthoides, collected in the Argentine South Atlantic coast, was extracted with hot water and the crude product fractionated using cetrimide. The complexed material was subjected to fractional solubilization in solutions of increasing sodium chloride concentration and seven fractions were separated and analyzed. Structural analysis of the main fractions, those soluble in 3.0 and 4.0 M NaCl (yields 21.0% and 13.8%, respectively) and those insoluble in 4.0 M NaCl (yield 20.0%), indicated that this seaweed biosynthesizes (1 → 3)-linked α-d-mannans that are sulfated at positions 4 and 6. Three mannan fractions comprising considerable amounts of xylose were also isolated in very low total yield (2.0%). The fractions that were soluble in 3.0 and 4.0 M NaCl showed low antiherpetic activity whereas this activity was considerable for the fraction solubilized in 2.0 M NaCl (yield 0.5%) which contained single stubs of β-d-xylose. A xylan, soluble in cetrimide solution, containing (1 → 3, 1 → 4)-linked β-d-xylose residues, was also isolated in minor amount. © 2009 Elsevier Ltd. All rights reserved. |
| author |
Pérez Recalde, María Mercedes Noseda, Miguel D. Pujol, Carlos Alberto Carlucci, María Josefina Matulewicz, María Cristina |
| author_facet |
Pérez Recalde, María Mercedes Noseda, Miguel D. Pujol, Carlos Alberto Carlucci, María Josefina Matulewicz, María Cristina |
| author_sort |
Pérez Recalde, María Mercedes |
| title |
Sulfated mannans from the red seaweed Nemalion helminthoides of the South Atlantic |
| title_short |
Sulfated mannans from the red seaweed Nemalion helminthoides of the South Atlantic |
| title_full |
Sulfated mannans from the red seaweed Nemalion helminthoides of the South Atlantic |
| title_fullStr |
Sulfated mannans from the red seaweed Nemalion helminthoides of the South Atlantic |
| title_full_unstemmed |
Sulfated mannans from the red seaweed Nemalion helminthoides of the South Atlantic |
| title_sort |
sulfated mannans from the red seaweed nemalion helminthoides of the south atlantic |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319422_v70_n8_p1062_PerezRecalde http://hdl.handle.net/20.500.12110/paper_00319422_v70_n8_p1062_PerezRecalde |
| work_keys_str_mv |
AT perezrecaldemariamercedes sulfatedmannansfromtheredseaweednemalionhelminthoidesofthesouthatlantic AT nosedamigueld sulfatedmannansfromtheredseaweednemalionhelminthoidesofthesouthatlantic AT pujolcarlosalberto sulfatedmannansfromtheredseaweednemalionhelminthoidesofthesouthatlantic AT carluccimariajosefina sulfatedmannansfromtheredseaweednemalionhelminthoidesofthesouthatlantic AT matulewiczmariacristina sulfatedmannansfromtheredseaweednemalionhelminthoidesofthesouthatlantic |
| _version_ |
1768544629471313920 |