In vitro anti-herpetic activity of sulfated polysaccharide fractions from Caulerpa racemosa
Sulfated heteropolysaccharide fractions isolated from the marine green algae Caulerpa racemosa were partially characterised by IR, GC, GC-MS and SEC. The hot water extracted polysaccharide fractions show anti-herpectic activity. A sulfated polysaccharide fraction was isolated from the hot water extr...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319422_v65_n23_p3151_Ghosh http://hdl.handle.net/20.500.12110/paper_00319422_v65_n23_p3151_Ghosh |
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paper:paper_00319422_v65_n23_p3151_Ghosh2023-06-08T14:59:37Z In vitro anti-herpetic activity of sulfated polysaccharide fractions from Caulerpa racemosa Antiviral activity Caulerpa racemosa Caulerpaceae Herpes simplex virus Sulfated heteropolysaccharide aciclovir arabinose Caulerpa racemosa extract dextran galactose glucose polymer polysaccharide sugar sulfate unclassified drug water xylose animal cell anticoagulation antiviral activity article Caulerpa racemosa concentration response controlled study cytotoxicity drug activity fractionation gel permeation chromatography green alga Herpes simplex virus 1 Herpes simplex virus 2 hydrodynamics infrared spectroscopy linkage analysis methylation nonhuman sulfation Vero cell Animals Anticoagulants Antiviral Agents Caulerpa Cell Survival Cercopithecus aethiops Chromatography, Gel Polysaccharides Simplexvirus Spectrophotometry, Infrared Vero Cells algae Caulerpa Caulerpa racemosa Caulerpaceae Chlorophyta Human herpesvirus 1 Simplexvirus Sulfated heteropolysaccharide fractions isolated from the marine green algae Caulerpa racemosa were partially characterised by IR, GC, GC-MS and SEC. The hot water extracted polysaccharide fractions show anti-herpectic activity. A sulfated polysaccharide fraction was isolated from the hot water extract of the green alga Caulerpa racemosa and designated HWE. This polymer, which contained galactose, glucose, arabinose and xylose as the major component sugars, had [α] D 30 +46.2° in water and contained 9% sulfate hemiester groups. Sugar linkage analysis indicates that HWE was branched and mainly contained 1,3- and 1,3,6-linked galactose, 1,3,4-linked arabinose, 1,4-linked glucose and terminal- and 1,4-linked xylose residues. Sulfation was deduced from infrared spectroscopy and methylation analysis to occur on O-6 of galactose and O-3 of arabinose. The native polysaccharide could be fractionated by size exclusion chromatography into two overlapping fractions and the major fraction has a hydrodynamic volume similar to that of 70 kDa dextran. HWE was a selective inhibitor of reference strains and TK - acyclovir-resistant strains of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in Vero cells, with antiviral effective concentration 50% (EC 50 ) values in the range of 2.2-4.2 μg/ml and lacking cytotoxic effects. Furthermore, HWE did not exhibit anticoagulant properties at concentrations near the EC 50 . © 2004 Elsevier Ltd. All rights reserved. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319422_v65_n23_p3151_Ghosh http://hdl.handle.net/20.500.12110/paper_00319422_v65_n23_p3151_Ghosh |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiviral activity Caulerpa racemosa Caulerpaceae Herpes simplex virus Sulfated heteropolysaccharide aciclovir arabinose Caulerpa racemosa extract dextran galactose glucose polymer polysaccharide sugar sulfate unclassified drug water xylose animal cell anticoagulation antiviral activity article Caulerpa racemosa concentration response controlled study cytotoxicity drug activity fractionation gel permeation chromatography green alga Herpes simplex virus 1 Herpes simplex virus 2 hydrodynamics infrared spectroscopy linkage analysis methylation nonhuman sulfation Vero cell Animals Anticoagulants Antiviral Agents Caulerpa Cell Survival Cercopithecus aethiops Chromatography, Gel Polysaccharides Simplexvirus Spectrophotometry, Infrared Vero Cells algae Caulerpa Caulerpa racemosa Caulerpaceae Chlorophyta Human herpesvirus 1 Simplexvirus |
| spellingShingle |
Antiviral activity Caulerpa racemosa Caulerpaceae Herpes simplex virus Sulfated heteropolysaccharide aciclovir arabinose Caulerpa racemosa extract dextran galactose glucose polymer polysaccharide sugar sulfate unclassified drug water xylose animal cell anticoagulation antiviral activity article Caulerpa racemosa concentration response controlled study cytotoxicity drug activity fractionation gel permeation chromatography green alga Herpes simplex virus 1 Herpes simplex virus 2 hydrodynamics infrared spectroscopy linkage analysis methylation nonhuman sulfation Vero cell Animals Anticoagulants Antiviral Agents Caulerpa Cell Survival Cercopithecus aethiops Chromatography, Gel Polysaccharides Simplexvirus Spectrophotometry, Infrared Vero Cells algae Caulerpa Caulerpa racemosa Caulerpaceae Chlorophyta Human herpesvirus 1 Simplexvirus In vitro anti-herpetic activity of sulfated polysaccharide fractions from Caulerpa racemosa |
| topic_facet |
Antiviral activity Caulerpa racemosa Caulerpaceae Herpes simplex virus Sulfated heteropolysaccharide aciclovir arabinose Caulerpa racemosa extract dextran galactose glucose polymer polysaccharide sugar sulfate unclassified drug water xylose animal cell anticoagulation antiviral activity article Caulerpa racemosa concentration response controlled study cytotoxicity drug activity fractionation gel permeation chromatography green alga Herpes simplex virus 1 Herpes simplex virus 2 hydrodynamics infrared spectroscopy linkage analysis methylation nonhuman sulfation Vero cell Animals Anticoagulants Antiviral Agents Caulerpa Cell Survival Cercopithecus aethiops Chromatography, Gel Polysaccharides Simplexvirus Spectrophotometry, Infrared Vero Cells algae Caulerpa Caulerpa racemosa Caulerpaceae Chlorophyta Human herpesvirus 1 Simplexvirus |
| description |
Sulfated heteropolysaccharide fractions isolated from the marine green algae Caulerpa racemosa were partially characterised by IR, GC, GC-MS and SEC. The hot water extracted polysaccharide fractions show anti-herpectic activity. A sulfated polysaccharide fraction was isolated from the hot water extract of the green alga Caulerpa racemosa and designated HWE. This polymer, which contained galactose, glucose, arabinose and xylose as the major component sugars, had [α] D 30 +46.2° in water and contained 9% sulfate hemiester groups. Sugar linkage analysis indicates that HWE was branched and mainly contained 1,3- and 1,3,6-linked galactose, 1,3,4-linked arabinose, 1,4-linked glucose and terminal- and 1,4-linked xylose residues. Sulfation was deduced from infrared spectroscopy and methylation analysis to occur on O-6 of galactose and O-3 of arabinose. The native polysaccharide could be fractionated by size exclusion chromatography into two overlapping fractions and the major fraction has a hydrodynamic volume similar to that of 70 kDa dextran. HWE was a selective inhibitor of reference strains and TK - acyclovir-resistant strains of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in Vero cells, with antiviral effective concentration 50% (EC 50 ) values in the range of 2.2-4.2 μg/ml and lacking cytotoxic effects. Furthermore, HWE did not exhibit anticoagulant properties at concentrations near the EC 50 . © 2004 Elsevier Ltd. All rights reserved. |
| title |
In vitro anti-herpetic activity of sulfated polysaccharide fractions from Caulerpa racemosa |
| title_short |
In vitro anti-herpetic activity of sulfated polysaccharide fractions from Caulerpa racemosa |
| title_full |
In vitro anti-herpetic activity of sulfated polysaccharide fractions from Caulerpa racemosa |
| title_fullStr |
In vitro anti-herpetic activity of sulfated polysaccharide fractions from Caulerpa racemosa |
| title_full_unstemmed |
In vitro anti-herpetic activity of sulfated polysaccharide fractions from Caulerpa racemosa |
| title_sort |
in vitro anti-herpetic activity of sulfated polysaccharide fractions from caulerpa racemosa |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319422_v65_n23_p3151_Ghosh http://hdl.handle.net/20.500.12110/paper_00319422_v65_n23_p3151_Ghosh |
| _version_ |
1768546434476408832 |