Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination
In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v149_n2_p286_Roqueiro http://hdl.handle.net/20.500.12110/paper_00319317_v149_n2_p286_Roqueiro |
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paper:paper_00319317_v149_n2_p286_Roqueiro2023-06-08T14:58:59Z Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination Roqueiro, Gonzalo Causin, Humberto Fabio Maroder, Horacio Luis Salix antioxidant ascorbate peroxidase catalase guaiacol peroxidase malonaldehyde peroxidase reactive oxygen metabolite superoxide dismutase analysis of variance article chloroplast cotyledon germination growth, development and aging light lipid peroxidation metabolism oxidation reduction reaction physiological stress plant leaf plant root plant seed radiation exposure seedling time transmission electron microscopy ultrastructure willow Analysis of Variance Antioxidants Ascorbate Peroxidases Catalase Chloroplasts Cotyledon Germination Light Lipid Peroxidation Malondialdehyde Microscopy, Electron, Transmission Oxidation-Reduction Peroxidase Plant Leaves Plant Roots Reactive Oxygen Species Salix Seedling Seeds Stress, Physiological Superoxide Dismutase Time Factors In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons. © 2013 Scandinavian Plant Physiology Society. Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Causin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v149_n2_p286_Roqueiro http://hdl.handle.net/20.500.12110/paper_00319317_v149_n2_p286_Roqueiro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Salix antioxidant ascorbate peroxidase catalase guaiacol peroxidase malonaldehyde peroxidase reactive oxygen metabolite superoxide dismutase analysis of variance article chloroplast cotyledon germination growth, development and aging light lipid peroxidation metabolism oxidation reduction reaction physiological stress plant leaf plant root plant seed radiation exposure seedling time transmission electron microscopy ultrastructure willow Analysis of Variance Antioxidants Ascorbate Peroxidases Catalase Chloroplasts Cotyledon Germination Light Lipid Peroxidation Malondialdehyde Microscopy, Electron, Transmission Oxidation-Reduction Peroxidase Plant Leaves Plant Roots Reactive Oxygen Species Salix Seedling Seeds Stress, Physiological Superoxide Dismutase Time Factors |
| spellingShingle |
Salix antioxidant ascorbate peroxidase catalase guaiacol peroxidase malonaldehyde peroxidase reactive oxygen metabolite superoxide dismutase analysis of variance article chloroplast cotyledon germination growth, development and aging light lipid peroxidation metabolism oxidation reduction reaction physiological stress plant leaf plant root plant seed radiation exposure seedling time transmission electron microscopy ultrastructure willow Analysis of Variance Antioxidants Ascorbate Peroxidases Catalase Chloroplasts Cotyledon Germination Light Lipid Peroxidation Malondialdehyde Microscopy, Electron, Transmission Oxidation-Reduction Peroxidase Plant Leaves Plant Roots Reactive Oxygen Species Salix Seedling Seeds Stress, Physiological Superoxide Dismutase Time Factors Roqueiro, Gonzalo Causin, Humberto Fabio Maroder, Horacio Luis Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination |
| topic_facet |
Salix antioxidant ascorbate peroxidase catalase guaiacol peroxidase malonaldehyde peroxidase reactive oxygen metabolite superoxide dismutase analysis of variance article chloroplast cotyledon germination growth, development and aging light lipid peroxidation metabolism oxidation reduction reaction physiological stress plant leaf plant root plant seed radiation exposure seedling time transmission electron microscopy ultrastructure willow Analysis of Variance Antioxidants Ascorbate Peroxidases Catalase Chloroplasts Cotyledon Germination Light Lipid Peroxidation Malondialdehyde Microscopy, Electron, Transmission Oxidation-Reduction Peroxidase Plant Leaves Plant Roots Reactive Oxygen Species Salix Seedling Seeds Stress, Physiological Superoxide Dismutase Time Factors |
| description |
In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons. © 2013 Scandinavian Plant Physiology Society. |
| author |
Roqueiro, Gonzalo Causin, Humberto Fabio Maroder, Horacio Luis |
| author_facet |
Roqueiro, Gonzalo Causin, Humberto Fabio Maroder, Horacio Luis |
| author_sort |
Roqueiro, Gonzalo |
| title |
Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination |
| title_short |
Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination |
| title_full |
Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination |
| title_fullStr |
Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination |
| title_full_unstemmed |
Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination |
| title_sort |
willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v149_n2_p286_Roqueiro http://hdl.handle.net/20.500.12110/paper_00319317_v149_n2_p286_Roqueiro |
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