Changes in motility and induction of enzymatic activity by nitrogen and phosphate deficiency in benthic Halamphora luciae (Bacillariophyceae) from Argentina

Cells of Halamphora luciae were isolated from the mudflat of the Bahía Blanca estuary. Unialgal axenic cultures were grown in f/2 with limiting concentrations of N or P and in f/2 medium deprived of one or both macronutrients. On the one hand, limited macronutrient supply did not affect growth rate,...

Descripción completa

Detalles Bibliográficos
Autores principales:	Maidana, Nora Irene, Matulewicz, María Cristina, Rodriguez, María Cecilia
Publicado:	2016
Materias:	Alkaline phosphatase Biovolume EPS Frustule morphology Halamphora Motility Nitrogen and phosphorus deficiency Protease
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318884_v55_n5_p493_Daglio http://hdl.handle.net/20.500.12110/paper_00318884_v55_n5_p493_Daglio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00318884_v55_n5_p493_Daglio
record_format	dspace
spelling	paper:paper_00318884_v55_n5_p493_Daglio2023-06-08T14:57:37Z Changes in motility and induction of enzymatic activity by nitrogen and phosphate deficiency in benthic Halamphora luciae (Bacillariophyceae) from Argentina Maidana, Nora Irene Matulewicz, María Cristina Rodriguez, María Cecilia Alkaline phosphatase Biovolume EPS Frustule morphology Halamphora Motility Nitrogen and phosphorus deficiency Protease Cells of Halamphora luciae were isolated from the mudflat of the Bahía Blanca estuary. Unialgal axenic cultures were grown in f/2 with limiting concentrations of N or P and in f/2 medium deprived of one or both macronutrients. On the one hand, limited macronutrient supply did not affect growth rate, but final cell yield was lower than in the complete medium. On the other hand, N or P deprivation reduced growth rates as well as final cell yield in the stationary phase. P deficiency enhanced the secretion of soluble extracellular polymeric substances (EPS), the percentage and speed of gliding cells, as well as the activity of alkaline phosphatase, especially after a previous prolonged P starvation. Regarding the morphology of the frustules, P deprivation reduced areola size but did not alter cell biovolume. N deficiency had a deleterious effect on the motility of the cells, and reduced the secretion of soluble EPS and cell biovolume. After incubation in N-deprived media for 10 days, cells exhibited enhanced protease activity. © 2016 International Phycological Society. Fil:Maidana, N.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318884_v55_n5_p493_Daglio http://hdl.handle.net/20.500.12110/paper_00318884_v55_n5_p493_Daglio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Alkaline phosphatase Biovolume EPS Frustule morphology Halamphora Motility Nitrogen and phosphorus deficiency Protease
spellingShingle	Alkaline phosphatase Biovolume EPS Frustule morphology Halamphora Motility Nitrogen and phosphorus deficiency Protease Maidana, Nora Irene Matulewicz, María Cristina Rodriguez, María Cecilia Changes in motility and induction of enzymatic activity by nitrogen and phosphate deficiency in benthic Halamphora luciae (Bacillariophyceae) from Argentina
topic_facet	Alkaline phosphatase Biovolume EPS Frustule morphology Halamphora Motility Nitrogen and phosphorus deficiency Protease
description	Cells of Halamphora luciae were isolated from the mudflat of the Bahía Blanca estuary. Unialgal axenic cultures were grown in f/2 with limiting concentrations of N or P and in f/2 medium deprived of one or both macronutrients. On the one hand, limited macronutrient supply did not affect growth rate, but final cell yield was lower than in the complete medium. On the other hand, N or P deprivation reduced growth rates as well as final cell yield in the stationary phase. P deficiency enhanced the secretion of soluble extracellular polymeric substances (EPS), the percentage and speed of gliding cells, as well as the activity of alkaline phosphatase, especially after a previous prolonged P starvation. Regarding the morphology of the frustules, P deprivation reduced areola size but did not alter cell biovolume. N deficiency had a deleterious effect on the motility of the cells, and reduced the secretion of soluble EPS and cell biovolume. After incubation in N-deprived media for 10 days, cells exhibited enhanced protease activity. © 2016 International Phycological Society.
author	Maidana, Nora Irene Matulewicz, María Cristina Rodriguez, María Cecilia
author_facet	Maidana, Nora Irene Matulewicz, María Cristina Rodriguez, María Cecilia
author_sort	Maidana, Nora Irene
title	Changes in motility and induction of enzymatic activity by nitrogen and phosphate deficiency in benthic Halamphora luciae (Bacillariophyceae) from Argentina
title_short	Changes in motility and induction of enzymatic activity by nitrogen and phosphate deficiency in benthic Halamphora luciae (Bacillariophyceae) from Argentina
title_full	Changes in motility and induction of enzymatic activity by nitrogen and phosphate deficiency in benthic Halamphora luciae (Bacillariophyceae) from Argentina
title_fullStr	Changes in motility and induction of enzymatic activity by nitrogen and phosphate deficiency in benthic Halamphora luciae (Bacillariophyceae) from Argentina
title_full_unstemmed	Changes in motility and induction of enzymatic activity by nitrogen and phosphate deficiency in benthic Halamphora luciae (Bacillariophyceae) from Argentina
title_sort	changes in motility and induction of enzymatic activity by nitrogen and phosphate deficiency in benthic halamphora luciae (bacillariophyceae) from argentina
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318884_v55_n5_p493_Daglio http://hdl.handle.net/20.500.12110/paper_00318884_v55_n5_p493_Daglio
work_keys_str_mv	AT maidananorairene changesinmotilityandinductionofenzymaticactivitybynitrogenandphosphatedeficiencyinbenthichalamphoraluciaebacillariophyceaefromargentina AT matulewiczmariacristina changesinmotilityandinductionofenzymaticactivitybynitrogenandphosphatedeficiencyinbenthichalamphoraluciaebacillariophyceaefromargentina AT rodriguezmariacecilia changesinmotilityandinductionofenzymaticactivitybynitrogenandphosphatedeficiencyinbenthichalamphoraluciaebacillariophyceaefromargentina
_version_	1768544167924858880

Changes in motility and induction of enzymatic activity by nitrogen and phosphate deficiency in benthic Halamphora luciae (Bacillariophyceae) from Argentina

Ejemplares similares