Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)

The effect of copper-imposed oxidative stress on isocitrate lyase (ICL) activity was studied in a Chlamydomonas reinhardtii wild type and a copper-tolerant pleiotropic mutant strain. Suspension batch bioassays were performed adding copper chloride under moderate irradiation. Two harvest points were...

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Autores principales: Sztrum, Abelardo Andrés, Sabatini, Sebastián Eduardo, Rodriguez, María Cecilia
Publicado: 2012
Materias:
Ascorbate peroxidase
Catalase
Chlamydomonas reinhardtii
Copper
Isocitrate lyase
Lipid peroxidation
Oxidative stress
Chlamydomonadales
Chlorophyceae
Mastigophora (flagellates)
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318884_v51_n2_p135_Sztrum
http://hdl.handle.net/20.500.12110/paper_00318884_v51_n2_p135_Sztrum
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00318884_v51_n2_p135_Sztrum
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spelling paper:paper_00318884_v51_n2_p135_Sztrum2023-06-08T14:57:37Z Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) Sztrum, Abelardo Andrés Sabatini, Sebastián Eduardo Rodriguez, María Cecilia Ascorbate peroxidase Catalase Chlamydomonas reinhardtii Copper Isocitrate lyase Lipid peroxidation Oxidative stress Chlamydomonadales Chlamydomonas reinhardtii Chlorophyceae Mastigophora (flagellates) The effect of copper-imposed oxidative stress on isocitrate lyase (ICL) activity was studied in a Chlamydomonas reinhardtii wild type and a copper-tolerant pleiotropic mutant strain. Suspension batch bioassays were performed adding copper chloride under moderate irradiation. Two harvest points were selected to represent the acute and restoration phases and oxidative stress indicators were measured: antioxidative metabolites, phenolic compounds, ascorbic acid, abundance of thiobarbituric reactive substances, and activities of detoxifying enzymes such as catalase and ascorbate peroxidase. Copper stress under moderate irradiance favours acetate assimilation through enhanced ICL activity, especially in the acute phase. Though the mutant strain exhibited at least twofold ICL activity in the acute phase compared to the wild type, there was no significant difference in copper tolerance between strains. The higher ICL activity under copper stress may be an adaptation to promote mixotrophic organic carbon assimilation when photosynthetic carbon dioxide fixation is impaired. Thus, the higher ICL activity has the potential to be a useful indicator in acetate-flagellate bioassays. Fil:Sztrum, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sabatini, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318884_v51_n2_p135_Sztrum http://hdl.handle.net/20.500.12110/paper_00318884_v51_n2_p135_Sztrum
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ascorbate peroxidase
Catalase
Chlamydomonas reinhardtii
Copper
Isocitrate lyase
Lipid peroxidation
Oxidative stress
Chlamydomonadales
Chlamydomonas reinhardtii
Chlorophyceae
Mastigophora (flagellates)
spellingShingle Ascorbate peroxidase
Catalase
Chlamydomonas reinhardtii
Copper
Isocitrate lyase
Lipid peroxidation
Oxidative stress
Chlamydomonadales
Chlamydomonas reinhardtii
Chlorophyceae
Mastigophora (flagellates)
Sztrum, Abelardo Andrés
Sabatini, Sebastián Eduardo
Rodriguez, María Cecilia
Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
topic_facet Ascorbate peroxidase
Catalase
Chlamydomonas reinhardtii
Copper
Isocitrate lyase
Lipid peroxidation
Oxidative stress
Chlamydomonadales
Chlamydomonas reinhardtii
Chlorophyceae
Mastigophora (flagellates)
description The effect of copper-imposed oxidative stress on isocitrate lyase (ICL) activity was studied in a Chlamydomonas reinhardtii wild type and a copper-tolerant pleiotropic mutant strain. Suspension batch bioassays were performed adding copper chloride under moderate irradiation. Two harvest points were selected to represent the acute and restoration phases and oxidative stress indicators were measured: antioxidative metabolites, phenolic compounds, ascorbic acid, abundance of thiobarbituric reactive substances, and activities of detoxifying enzymes such as catalase and ascorbate peroxidase. Copper stress under moderate irradiance favours acetate assimilation through enhanced ICL activity, especially in the acute phase. Though the mutant strain exhibited at least twofold ICL activity in the acute phase compared to the wild type, there was no significant difference in copper tolerance between strains. The higher ICL activity under copper stress may be an adaptation to promote mixotrophic organic carbon assimilation when photosynthetic carbon dioxide fixation is impaired. Thus, the higher ICL activity has the potential to be a useful indicator in acetate-flagellate bioassays.
author Sztrum, Abelardo Andrés
Sabatini, Sebastián Eduardo
Rodriguez, María Cecilia
author_facet Sztrum, Abelardo Andrés
Sabatini, Sebastián Eduardo
Rodriguez, María Cecilia
author_sort Sztrum, Abelardo Andrés
title Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
title_short Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
title_full Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
title_fullStr Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
title_full_unstemmed Isocitrate lyase activity and antioxidant responses in copper-stressed cultures of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
title_sort isocitrate lyase activity and antioxidant responses in copper-stressed cultures of chlamydomonas reinhardtii (volvocales, chlorophyceae)
publishDate 2012
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318884_v51_n2_p135_Sztrum
http://hdl.handle.net/20.500.12110/paper_00318884_v51_n2_p135_Sztrum
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AT sabatinisebastianeduardo isocitratelyaseactivityandantioxidantresponsesincopperstressedculturesofchlamydomonasreinhardtiivolvocaleschlorophyceae
AT rodriguezmariacecilia isocitratelyaseactivityandantioxidantresponsesincopperstressedculturesofchlamydomonasreinhardtiivolvocaleschlorophyceae
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