Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi

We report the molecular characterization of a middle repetitive DNA sequence, named C6, isolated from the Trypanosoma cruzi genome. C6 appears to be a composite repeated element since 3 subregions may be defined within it on the basis of sequence similarities with other T. cruzi genomic sequences. S...

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Publicado:	1997
Materias:	Diagnostic tool Genomic distribution Polymorphic marker Sequence analysis Trypanosoma cruzi repetitive DNA article dna sequence gene location nonhuman nucleotide repeat parasite identification priority journal sequence analysis sequence homology species difference trypanosoma cruzi Animals Base Sequence Blotting, Southern Chagas Disease DNA, Protozoan Electrophoresis, Gel, Pulsed-Field Genome, Protozoan Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Species Specificity Trypanosoma cruzi Protozoa Trypanosoma
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v115_n6_p563_Araya http://hdl.handle.net/20.500.12110/paper_00311820_v115_n6_p563_Araya
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00311820_v115_n6_p563_Araya
record_format	dspace
spelling	paper:paper_00311820_v115_n6_p563_Araya2023-06-08T14:56:55Z Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi Diagnostic tool Genomic distribution Polymorphic marker Sequence analysis Trypanosoma cruzi repetitive DNA article dna sequence gene location nonhuman nucleotide repeat parasite identification priority journal sequence analysis sequence homology species difference trypanosoma cruzi Animals Base Sequence Blotting, Southern Chagas Disease DNA, Protozoan Electrophoresis, Gel, Pulsed-Field Genome, Protozoan Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Species Specificity Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi We report the molecular characterization of a middle repetitive DNA sequence, named C6, isolated from the Trypanosoma cruzi genome. C6 appears to be a composite repeated element since 3 subregions may be defined within it on the basis of sequence similarities with other T. cruzi genomic sequences. Sequences homologous to C6 are interspersed in the genome and can be mapped out on most chromosomal bands of different T. cruzi. strains. The copy number of the C6 element is about 1000 per haploid genome. Given the species specificity and different genomic distribution of C6 homologous sequences among the T. cruzi strains the C6 element could be a useful probe for diagnosis and typing of parasites. C6 is a polymorphic marker with potential as a tool for physical mapping of the T. cruzi genome. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v115_n6_p563_Araya http://hdl.handle.net/20.500.12110/paper_00311820_v115_n6_p563_Araya
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Diagnostic tool Genomic distribution Polymorphic marker Sequence analysis Trypanosoma cruzi repetitive DNA article dna sequence gene location nonhuman nucleotide repeat parasite identification priority journal sequence analysis sequence homology species difference trypanosoma cruzi Animals Base Sequence Blotting, Southern Chagas Disease DNA, Protozoan Electrophoresis, Gel, Pulsed-Field Genome, Protozoan Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Species Specificity Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi
spellingShingle	Diagnostic tool Genomic distribution Polymorphic marker Sequence analysis Trypanosoma cruzi repetitive DNA article dna sequence gene location nonhuman nucleotide repeat parasite identification priority journal sequence analysis sequence homology species difference trypanosoma cruzi Animals Base Sequence Blotting, Southern Chagas Disease DNA, Protozoan Electrophoresis, Gel, Pulsed-Field Genome, Protozoan Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Species Specificity Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi
topic_facet	Diagnostic tool Genomic distribution Polymorphic marker Sequence analysis Trypanosoma cruzi repetitive DNA article dna sequence gene location nonhuman nucleotide repeat parasite identification priority journal sequence analysis sequence homology species difference trypanosoma cruzi Animals Base Sequence Blotting, Southern Chagas Disease DNA, Protozoan Electrophoresis, Gel, Pulsed-Field Genome, Protozoan Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Species Specificity Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi
description	We report the molecular characterization of a middle repetitive DNA sequence, named C6, isolated from the Trypanosoma cruzi genome. C6 appears to be a composite repeated element since 3 subregions may be defined within it on the basis of sequence similarities with other T. cruzi genomic sequences. Sequences homologous to C6 are interspersed in the genome and can be mapped out on most chromosomal bands of different T. cruzi. strains. The copy number of the C6 element is about 1000 per haploid genome. Given the species specificity and different genomic distribution of C6 homologous sequences among the T. cruzi strains the C6 element could be a useful probe for diagnosis and typing of parasites. C6 is a polymorphic marker with potential as a tool for physical mapping of the T. cruzi genome.
title	Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi
title_short	Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi
title_full	Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi
title_fullStr	Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi
title_full_unstemmed	Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi
title_sort	characterization of an interspersed repetitive dna element in the genome of trypanosoma cruzi
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00311820_v115_n6_p563_Araya http://hdl.handle.net/20.500.12110/paper_00311820_v115_n6_p563_Araya
_version_	1768542929594351616

Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi

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