Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells

The polyphenol curcumin (diferuloylmethane) is the active componenet of the spice plant Curcuma longa and has been shown to exert multiple actions on mammalian cells. We have studied its effect on folliculostellate (FS) TtT/GF mouse pituitary cells, representative of a multifunctional, endocrine ina...

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Publicado:	2010
Materias:	Apoptosis Cell cycle Curcumin Folliculostellate pituitary cells Interleukin-6 Nuclear factor-κB Proliferation Tlr4 Vascular endothelial growth factor 7 aminodactinomycin agar caspase 3 curcumin cyclin D1 DNA endotoxin fluorescein isothiocyanate immunoglobulin enhancer binding protein interleukin 6 lipocortin 5 messenger RNA protein bcl 2 toll like receptor 4 vasculotropin A angiogenesis animal cell antineoplastic activity apoptosis article cell culture cell cycle arrest cell cycle G2 phase cell cycle M phase cell death cell growth cell proliferation cell type colony formation controlled study cytokine production cytokine release DNA fragmentation drug inhibition endocrine cell fluorescence activated cell sorting growth inhibition hypophysis cell immune response immunohistochemistry monolayer culture nonhuman nucleotide sequence pituitary folliculostellate cell priority journal protein cleavage protein expression receptor down regulation RNA synthesis Animals Anti-Inflammatory Agents, Non-Steroidal Cell Cycle Cell Division Cell Line, Tumor Male Mice Pituitary Gland, Anterior Pituitary Neoplasms Rats Rats, Sprague-Dawley RNA, Messenger Vascular Endothelial Growth Factor A
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v91_n2_p200_Schaaf http://hdl.handle.net/20.500.12110/paper_00283835_v91_n2_p200_Schaaf
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00283835_v91_n2_p200_Schaaf2023-06-08T14:55:06Z Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells Apoptosis Cell cycle Curcumin Folliculostellate pituitary cells Interleukin-6 Nuclear factor-κB Proliferation Tlr4 Vascular endothelial growth factor 7 aminodactinomycin agar caspase 3 curcumin cyclin D1 DNA endotoxin fluorescein isothiocyanate immunoglobulin enhancer binding protein interleukin 6 lipocortin 5 messenger RNA protein bcl 2 toll like receptor 4 vasculotropin A angiogenesis animal cell antineoplastic activity apoptosis article cell culture cell cycle arrest cell cycle G2 phase cell cycle M phase cell death cell growth cell proliferation cell type colony formation controlled study cytokine production cytokine release DNA fragmentation drug inhibition endocrine cell fluorescence activated cell sorting growth inhibition hypophysis cell immune response immunohistochemistry monolayer culture nonhuman nucleotide sequence pituitary folliculostellate cell priority journal protein cleavage protein expression receptor down regulation RNA synthesis Animals Anti-Inflammatory Agents, Non-Steroidal Apoptosis Cell Cycle Cell Division Cell Line, Tumor Curcumin Interleukin-6 Male Mice Pituitary Gland, Anterior Pituitary Neoplasms Rats Rats, Sprague-Dawley RNA, Messenger Vascular Endothelial Growth Factor A The polyphenol curcumin (diferuloylmethane) is the active componenet of the spice plant Curcuma longa and has been shown to exert multiple actions on mammalian cells. We have studied its effect on folliculostellate (FS) TtT/GF mouse pituitary cells, representative of a multifunctional, endocrine inactive cell type of the anterior pituitary. Proliferation of TtT/GF cells was inhibited by curcumin in a monolayer cell culture and in the colony formation assay in soft agar. Fluorescence-activated cell-sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M accompanied by inhibition of cyclin D1 protein expression. Curcumin had a small effect on necrosis of TtT/GF cells, but it mainly stimulated apoptosis as demonstrated by FACS analysis (Annexin V-fluorescein isothiocyannate/7-aminoactinomycin D staining). Curcumin-induced apoptosis involved suppression of Bcl-2, stimulation of cleaved caspase-3 and induction of DNA fragmentation. Functional studies on FS cell-derived compounds showed that curcumin inhibited mRNA synthesis and release of angiogenic vascular endothelial growth factor-A (VEGF-A). Immune-like functions of FS cells were impaired since curcumin downregulated Toll-like receptor 4, reduced nuclear factor-κB expression and suppressed bacterial endotoxin-induced interleukin-6 (IL-6) secretion. The inhibitory action of curcumin on VEGF-A and IL-6 production was also found in primary rat pituitary cell cultures, in which FS cells are the only source of these proteins. The observed effects of curcumin on FS cell growth, apoptosis and functions may have therapeutic consequences for the intrapituitary regulation of hormone production and release as well as for pituitary tumor pathogenesis. Copyright © 2010 S. Karger AG. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v91_n2_p200_Schaaf http://hdl.handle.net/20.500.12110/paper_00283835_v91_n2_p200_Schaaf
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Apoptosis Cell cycle Curcumin Folliculostellate pituitary cells Interleukin-6 Nuclear factor-κB Proliferation Tlr4 Vascular endothelial growth factor 7 aminodactinomycin agar caspase 3 curcumin cyclin D1 DNA endotoxin fluorescein isothiocyanate immunoglobulin enhancer binding protein interleukin 6 lipocortin 5 messenger RNA protein bcl 2 toll like receptor 4 vasculotropin A angiogenesis animal cell antineoplastic activity apoptosis article cell culture cell cycle arrest cell cycle G2 phase cell cycle M phase cell death cell growth cell proliferation cell type colony formation controlled study cytokine production cytokine release DNA fragmentation drug inhibition endocrine cell fluorescence activated cell sorting growth inhibition hypophysis cell immune response immunohistochemistry monolayer culture nonhuman nucleotide sequence pituitary folliculostellate cell priority journal protein cleavage protein expression receptor down regulation RNA synthesis Animals Anti-Inflammatory Agents, Non-Steroidal Apoptosis Cell Cycle Cell Division Cell Line, Tumor Curcumin Interleukin-6 Male Mice Pituitary Gland, Anterior Pituitary Neoplasms Rats Rats, Sprague-Dawley RNA, Messenger Vascular Endothelial Growth Factor A
spellingShingle	Apoptosis Cell cycle Curcumin Folliculostellate pituitary cells Interleukin-6 Nuclear factor-κB Proliferation Tlr4 Vascular endothelial growth factor 7 aminodactinomycin agar caspase 3 curcumin cyclin D1 DNA endotoxin fluorescein isothiocyanate immunoglobulin enhancer binding protein interleukin 6 lipocortin 5 messenger RNA protein bcl 2 toll like receptor 4 vasculotropin A angiogenesis animal cell antineoplastic activity apoptosis article cell culture cell cycle arrest cell cycle G2 phase cell cycle M phase cell death cell growth cell proliferation cell type colony formation controlled study cytokine production cytokine release DNA fragmentation drug inhibition endocrine cell fluorescence activated cell sorting growth inhibition hypophysis cell immune response immunohistochemistry monolayer culture nonhuman nucleotide sequence pituitary folliculostellate cell priority journal protein cleavage protein expression receptor down regulation RNA synthesis Animals Anti-Inflammatory Agents, Non-Steroidal Apoptosis Cell Cycle Cell Division Cell Line, Tumor Curcumin Interleukin-6 Male Mice Pituitary Gland, Anterior Pituitary Neoplasms Rats Rats, Sprague-Dawley RNA, Messenger Vascular Endothelial Growth Factor A Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
topic_facet	Apoptosis Cell cycle Curcumin Folliculostellate pituitary cells Interleukin-6 Nuclear factor-κB Proliferation Tlr4 Vascular endothelial growth factor 7 aminodactinomycin agar caspase 3 curcumin cyclin D1 DNA endotoxin fluorescein isothiocyanate immunoglobulin enhancer binding protein interleukin 6 lipocortin 5 messenger RNA protein bcl 2 toll like receptor 4 vasculotropin A angiogenesis animal cell antineoplastic activity apoptosis article cell culture cell cycle arrest cell cycle G2 phase cell cycle M phase cell death cell growth cell proliferation cell type colony formation controlled study cytokine production cytokine release DNA fragmentation drug inhibition endocrine cell fluorescence activated cell sorting growth inhibition hypophysis cell immune response immunohistochemistry monolayer culture nonhuman nucleotide sequence pituitary folliculostellate cell priority journal protein cleavage protein expression receptor down regulation RNA synthesis Animals Anti-Inflammatory Agents, Non-Steroidal Apoptosis Cell Cycle Cell Division Cell Line, Tumor Curcumin Interleukin-6 Male Mice Pituitary Gland, Anterior Pituitary Neoplasms Rats Rats, Sprague-Dawley RNA, Messenger Vascular Endothelial Growth Factor A
description	The polyphenol curcumin (diferuloylmethane) is the active componenet of the spice plant Curcuma longa and has been shown to exert multiple actions on mammalian cells. We have studied its effect on folliculostellate (FS) TtT/GF mouse pituitary cells, representative of a multifunctional, endocrine inactive cell type of the anterior pituitary. Proliferation of TtT/GF cells was inhibited by curcumin in a monolayer cell culture and in the colony formation assay in soft agar. Fluorescence-activated cell-sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M accompanied by inhibition of cyclin D1 protein expression. Curcumin had a small effect on necrosis of TtT/GF cells, but it mainly stimulated apoptosis as demonstrated by FACS analysis (Annexin V-fluorescein isothiocyannate/7-aminoactinomycin D staining). Curcumin-induced apoptosis involved suppression of Bcl-2, stimulation of cleaved caspase-3 and induction of DNA fragmentation. Functional studies on FS cell-derived compounds showed that curcumin inhibited mRNA synthesis and release of angiogenic vascular endothelial growth factor-A (VEGF-A). Immune-like functions of FS cells were impaired since curcumin downregulated Toll-like receptor 4, reduced nuclear factor-κB expression and suppressed bacterial endotoxin-induced interleukin-6 (IL-6) secretion. The inhibitory action of curcumin on VEGF-A and IL-6 production was also found in primary rat pituitary cell cultures, in which FS cells are the only source of these proteins. The observed effects of curcumin on FS cell growth, apoptosis and functions may have therapeutic consequences for the intrapituitary regulation of hormone production and release as well as for pituitary tumor pathogenesis. Copyright © 2010 S. Karger AG.
title	Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
title_short	Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
title_full	Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
title_fullStr	Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
title_full_unstemmed	Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
title_sort	curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v91_n2_p200_Schaaf http://hdl.handle.net/20.500.12110/paper_00283835_v91_n2_p200_Schaaf
_version_	1768545453259882496

Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells

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