Changes in fos expression in various brain regions during deoxycorticosterone acetate treatment: Relation to salt appetite, vasopressin mRNA and the mineralocorticoid receptor

Salt appetite, a conditioning factor for hypertension and cardiovascular diseases, is produced when high doses of mineralocorticoids are given to experimental animals. A commonly used procedure to identify neuronal activation is to determine the number of Fos-immunoreactive cells. In rats with estab...

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Publicado:	2001
Materias:	Adrenal steroid receptors Adrenal steroids Amygdala Fos Organum vasculosum laminae terminalis Paraventricular nucleus Salt appetite Supraoptic nucleus Vasopressin 4 aminobutyric acid receptor argipressin deoxycorticosterone acetate membrane receptor mineralocorticoid receptor protein fos sodium chloride vasopressin amygdaloid nucleus animal cell animal experiment animal tissue article brain region cardiovascular disease cell activation controlled study drug binding drug mechanism hypertension male nonhuman preoptic area preoptic nucleus priority journal protein expression rat sodium appetite stria terminalis supraoptic nucleus thalamus midline nucleus Non-programmatic Animals Appetite Arginine Vasopressin Brain Desoxycorticosterone Immunohistochemistry Male Proto-Oncogene Proteins c-fos Rats Rats, Sprague-Dawley Receptors, Mineralocorticoid RNA, Messenger Sodium Chloride Tissue Distribution
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v74_n6_p396_Pietranera http://hdl.handle.net/20.500.12110/paper_00283835_v74_n6_p396_Pietranera
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00283835_v74_n6_p396_Pietranera2023-06-08T14:55:04Z Changes in fos expression in various brain regions during deoxycorticosterone acetate treatment: Relation to salt appetite, vasopressin mRNA and the mineralocorticoid receptor Adrenal steroid receptors Adrenal steroids Amygdala Fos Organum vasculosum laminae terminalis Paraventricular nucleus Salt appetite Supraoptic nucleus Vasopressin 4 aminobutyric acid receptor argipressin deoxycorticosterone acetate membrane receptor mineralocorticoid receptor protein fos sodium chloride vasopressin amygdaloid nucleus animal cell animal experiment animal tissue article brain region cardiovascular disease cell activation controlled study drug binding drug mechanism hypertension male nonhuman preoptic area preoptic nucleus priority journal protein expression rat sodium appetite stria terminalis supraoptic nucleus thalamus midline nucleus Non-programmatic Animals Appetite Arginine Vasopressin Brain Desoxycorticosterone Immunohistochemistry Male Proto-Oncogene Proteins c-fos Rats Rats, Sprague-Dawley Receptors, Mineralocorticoid RNA, Messenger Sodium Chloride Tissue Distribution Salt appetite, a conditioning factor for hypertension and cardiovascular diseases, is produced when high doses of mineralocorticoids are given to experimental animals. A commonly used procedure to identify neuronal activation is to determine the number of Fos-immunoreactive cells. In rats with established salt appetite after 8 days of deoxycorticosterone acetate (DOCA) treatment, Fos-positive cells were studied in seven brain areas. Significant increases in Fos activity were recorded in the paraventricular (PVN) and supraoptic (SON) nuclei, median preoptic nucleus (MnPO), organum vasculosum of the lamina terminalis (OVLT), preoptic area (POA), bed nucleus of the stria terminalis (BNST) and amygdala (AMYG). In most of these areas, increased Fos expression was also observed early (2 h) after a single DOCA injection, well before salt appetite develops. Using a mineralocorticoid receptor (MR) antibody, we studied whether Fos-active regions also expressed MR. MR-positive cells were found in the OVLT, MnPO, AMYG and BNST, but not in the POA, PVN and SON. In the PVN and SON, nevertheless, prolonged or single DOCA treatment increased expression of mRNA for arginine vasopressin (AVP). The present demonstration of Fos activation, in conjunction with differential expression of MR and stimulation of AVP mRNA, suggests that a neuroanatomical pathway comprising the AMYG, osmosensitive brain regions and magnocellular nuclei becomes activated during DOCA effects on salt appetite. It is recognized, however, that DOCA effects may also depend on mechanisms and brain structures other than those considered in the present investigation. Since some Fos-positive regions were devoid of MR, a comprehensive view of DOCA-induced salt appetite should consider nonge-nomic pathways of steroid action, including the role of reduced DOC metabolites binding to GABAergic membrane receptors. Copyright © 2001 S. Karger AG, Basel. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v74_n6_p396_Pietranera http://hdl.handle.net/20.500.12110/paper_00283835_v74_n6_p396_Pietranera
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adrenal steroid receptors Adrenal steroids Amygdala Fos Organum vasculosum laminae terminalis Paraventricular nucleus Salt appetite Supraoptic nucleus Vasopressin 4 aminobutyric acid receptor argipressin deoxycorticosterone acetate membrane receptor mineralocorticoid receptor protein fos sodium chloride vasopressin amygdaloid nucleus animal cell animal experiment animal tissue article brain region cardiovascular disease cell activation controlled study drug binding drug mechanism hypertension male nonhuman preoptic area preoptic nucleus priority journal protein expression rat sodium appetite stria terminalis supraoptic nucleus thalamus midline nucleus Non-programmatic Animals Appetite Arginine Vasopressin Brain Desoxycorticosterone Immunohistochemistry Male Proto-Oncogene Proteins c-fos Rats Rats, Sprague-Dawley Receptors, Mineralocorticoid RNA, Messenger Sodium Chloride Tissue Distribution
spellingShingle	Adrenal steroid receptors Adrenal steroids Amygdala Fos Organum vasculosum laminae terminalis Paraventricular nucleus Salt appetite Supraoptic nucleus Vasopressin 4 aminobutyric acid receptor argipressin deoxycorticosterone acetate membrane receptor mineralocorticoid receptor protein fos sodium chloride vasopressin amygdaloid nucleus animal cell animal experiment animal tissue article brain region cardiovascular disease cell activation controlled study drug binding drug mechanism hypertension male nonhuman preoptic area preoptic nucleus priority journal protein expression rat sodium appetite stria terminalis supraoptic nucleus thalamus midline nucleus Non-programmatic Animals Appetite Arginine Vasopressin Brain Desoxycorticosterone Immunohistochemistry Male Proto-Oncogene Proteins c-fos Rats Rats, Sprague-Dawley Receptors, Mineralocorticoid RNA, Messenger Sodium Chloride Tissue Distribution Changes in fos expression in various brain regions during deoxycorticosterone acetate treatment: Relation to salt appetite, vasopressin mRNA and the mineralocorticoid receptor
topic_facet	Adrenal steroid receptors Adrenal steroids Amygdala Fos Organum vasculosum laminae terminalis Paraventricular nucleus Salt appetite Supraoptic nucleus Vasopressin 4 aminobutyric acid receptor argipressin deoxycorticosterone acetate membrane receptor mineralocorticoid receptor protein fos sodium chloride vasopressin amygdaloid nucleus animal cell animal experiment animal tissue article brain region cardiovascular disease cell activation controlled study drug binding drug mechanism hypertension male nonhuman preoptic area preoptic nucleus priority journal protein expression rat sodium appetite stria terminalis supraoptic nucleus thalamus midline nucleus Non-programmatic Animals Appetite Arginine Vasopressin Brain Desoxycorticosterone Immunohistochemistry Male Proto-Oncogene Proteins c-fos Rats Rats, Sprague-Dawley Receptors, Mineralocorticoid RNA, Messenger Sodium Chloride Tissue Distribution
description	Salt appetite, a conditioning factor for hypertension and cardiovascular diseases, is produced when high doses of mineralocorticoids are given to experimental animals. A commonly used procedure to identify neuronal activation is to determine the number of Fos-immunoreactive cells. In rats with established salt appetite after 8 days of deoxycorticosterone acetate (DOCA) treatment, Fos-positive cells were studied in seven brain areas. Significant increases in Fos activity were recorded in the paraventricular (PVN) and supraoptic (SON) nuclei, median preoptic nucleus (MnPO), organum vasculosum of the lamina terminalis (OVLT), preoptic area (POA), bed nucleus of the stria terminalis (BNST) and amygdala (AMYG). In most of these areas, increased Fos expression was also observed early (2 h) after a single DOCA injection, well before salt appetite develops. Using a mineralocorticoid receptor (MR) antibody, we studied whether Fos-active regions also expressed MR. MR-positive cells were found in the OVLT, MnPO, AMYG and BNST, but not in the POA, PVN and SON. In the PVN and SON, nevertheless, prolonged or single DOCA treatment increased expression of mRNA for arginine vasopressin (AVP). The present demonstration of Fos activation, in conjunction with differential expression of MR and stimulation of AVP mRNA, suggests that a neuroanatomical pathway comprising the AMYG, osmosensitive brain regions and magnocellular nuclei becomes activated during DOCA effects on salt appetite. It is recognized, however, that DOCA effects may also depend on mechanisms and brain structures other than those considered in the present investigation. Since some Fos-positive regions were devoid of MR, a comprehensive view of DOCA-induced salt appetite should consider nonge-nomic pathways of steroid action, including the role of reduced DOC metabolites binding to GABAergic membrane receptors. Copyright © 2001 S. Karger AG, Basel.
title	Changes in fos expression in various brain regions during deoxycorticosterone acetate treatment: Relation to salt appetite, vasopressin mRNA and the mineralocorticoid receptor
title_short	Changes in fos expression in various brain regions during deoxycorticosterone acetate treatment: Relation to salt appetite, vasopressin mRNA and the mineralocorticoid receptor
title_full	Changes in fos expression in various brain regions during deoxycorticosterone acetate treatment: Relation to salt appetite, vasopressin mRNA and the mineralocorticoid receptor
title_fullStr	Changes in fos expression in various brain regions during deoxycorticosterone acetate treatment: Relation to salt appetite, vasopressin mRNA and the mineralocorticoid receptor
title_full_unstemmed	Changes in fos expression in various brain regions during deoxycorticosterone acetate treatment: Relation to salt appetite, vasopressin mRNA and the mineralocorticoid receptor
title_sort	changes in fos expression in various brain regions during deoxycorticosterone acetate treatment: relation to salt appetite, vasopressin mrna and the mineralocorticoid receptor
publishDate	2001
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v74_n6_p396_Pietranera http://hdl.handle.net/20.500.12110/paper_00283835_v74_n6_p396_Pietranera
_version_	1768541880627232768

Changes in fos expression in various brain regions during deoxycorticosterone acetate treatment: Relation to salt appetite, vasopressin mRNA and the mineralocorticoid receptor

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