Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake
We have studied the role of mineralocorticoid receptors (MR) and glucocorticoid receptors (GR) on salt appetite developed by deoxycorticosterone acetate (DOCA) treated rats. To this end, we measured the effects of DOCA given on alternate days on (1) salt intake; (2) MR and GR in hippocampus (HIPPO),...
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1995
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v61_n2_p117_Vallee http://hdl.handle.net/20.500.12110/paper_00283835_v61_n2_p117_Vallee |
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paper:paper_00283835_v61_n2_p117_Vallee2023-06-08T14:55:02Z Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake Adrenal steroid receptors Adrenal steroids Ornithine decarboxylase activity Salt intake 15beta,16beta methylenemexrenone corticosterone deoxycorticosterone acetate dexamethasone glucocorticoid antagonist glucocorticoid receptor mifepristone mineralocorticoid antagonist mineralocorticoid receptor ornithine decarboxylase adrenalectomy amygdaloid nucleus animal experiment animal tissue article controlled study enzyme activity hippocampus hypothalamus male nonhuman priority journal rat receptor binding salt intake scatchard plot Amygdala Animal Appetite Brain Desoxycorticosterone Hippocampus Hypothalamus Male Mifepristone Ornithine Decarboxylase Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Receptors, Mineralocorticoid Sodium Chloride, Dietary Spironolactone We have studied the role of mineralocorticoid receptors (MR) and glucocorticoid receptors (GR) on salt appetite developed by deoxycorticosterone acetate (DOCA) treated rats. To this end, we measured the effects of DOCA given on alternate days on (1) salt intake; (2) MR and GR in hippocampus (HIPPO), amygdala (AMYG), and hypothalamus (HT); (3) the activity of ornithine decarboxylase (ODC), a OR-mediated response, and (4) the salt intake after treatment with the antiglucocorticoid RU 486 or the antimineralocorticoid ZK 91587. First, we demonstrated that 10 but not 1 mg DOCA induced natriogenesis. Forty-eight hours after adrenalectomy and 24 h after the last DOCA injection, 10 but not 1 mg hormone reduced binding to OR in HIPPO, AMYG, and HT. Both doses of DOCA also reduced the binding to MR in HIPPO, without changes in AMYG; in HT the 1-mg dose was without effect, but the natriogenic dose (10 mg) highly increased binding of [3H]-corticosterone to MR. Scatchard analysis demonstrated increased B(max) and K(d) values in the HT of DOCA-treated rats. Occupation of GR by DOCA did not stimulate the ODC activity, in contrast to the few-fold increment effected by the glucocorticoid dexamethasone. Also, administration of RU 486 did not inhibit the salt intake promoted by DOCA, in contrast to ZK 91587 which partly delayed the natriogenic effect of DOCA. It is suggested that brain MR are involved in the natriogenic effect of DOCA, whereas the role of GR is inconclusive. Although GR were occupied by DOCA, this compound did not show agonistic properties regarding induction of ODC nor antagonism of dexamethasone-induced ODC, indicating that DOCA (or its metabolites) rendered inactive GR. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v61_n2_p117_Vallee http://hdl.handle.net/20.500.12110/paper_00283835_v61_n2_p117_Vallee |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adrenal steroid receptors Adrenal steroids Ornithine decarboxylase activity Salt intake 15beta,16beta methylenemexrenone corticosterone deoxycorticosterone acetate dexamethasone glucocorticoid antagonist glucocorticoid receptor mifepristone mineralocorticoid antagonist mineralocorticoid receptor ornithine decarboxylase adrenalectomy amygdaloid nucleus animal experiment animal tissue article controlled study enzyme activity hippocampus hypothalamus male nonhuman priority journal rat receptor binding salt intake scatchard plot Amygdala Animal Appetite Brain Desoxycorticosterone Hippocampus Hypothalamus Male Mifepristone Ornithine Decarboxylase Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Receptors, Mineralocorticoid Sodium Chloride, Dietary Spironolactone |
| spellingShingle |
Adrenal steroid receptors Adrenal steroids Ornithine decarboxylase activity Salt intake 15beta,16beta methylenemexrenone corticosterone deoxycorticosterone acetate dexamethasone glucocorticoid antagonist glucocorticoid receptor mifepristone mineralocorticoid antagonist mineralocorticoid receptor ornithine decarboxylase adrenalectomy amygdaloid nucleus animal experiment animal tissue article controlled study enzyme activity hippocampus hypothalamus male nonhuman priority journal rat receptor binding salt intake scatchard plot Amygdala Animal Appetite Brain Desoxycorticosterone Hippocampus Hypothalamus Male Mifepristone Ornithine Decarboxylase Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Receptors, Mineralocorticoid Sodium Chloride, Dietary Spironolactone Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake |
| topic_facet |
Adrenal steroid receptors Adrenal steroids Ornithine decarboxylase activity Salt intake 15beta,16beta methylenemexrenone corticosterone deoxycorticosterone acetate dexamethasone glucocorticoid antagonist glucocorticoid receptor mifepristone mineralocorticoid antagonist mineralocorticoid receptor ornithine decarboxylase adrenalectomy amygdaloid nucleus animal experiment animal tissue article controlled study enzyme activity hippocampus hypothalamus male nonhuman priority journal rat receptor binding salt intake scatchard plot Amygdala Animal Appetite Brain Desoxycorticosterone Hippocampus Hypothalamus Male Mifepristone Ornithine Decarboxylase Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Receptors, Mineralocorticoid Sodium Chloride, Dietary Spironolactone |
| description |
We have studied the role of mineralocorticoid receptors (MR) and glucocorticoid receptors (GR) on salt appetite developed by deoxycorticosterone acetate (DOCA) treated rats. To this end, we measured the effects of DOCA given on alternate days on (1) salt intake; (2) MR and GR in hippocampus (HIPPO), amygdala (AMYG), and hypothalamus (HT); (3) the activity of ornithine decarboxylase (ODC), a OR-mediated response, and (4) the salt intake after treatment with the antiglucocorticoid RU 486 or the antimineralocorticoid ZK 91587. First, we demonstrated that 10 but not 1 mg DOCA induced natriogenesis. Forty-eight hours after adrenalectomy and 24 h after the last DOCA injection, 10 but not 1 mg hormone reduced binding to OR in HIPPO, AMYG, and HT. Both doses of DOCA also reduced the binding to MR in HIPPO, without changes in AMYG; in HT the 1-mg dose was without effect, but the natriogenic dose (10 mg) highly increased binding of [3H]-corticosterone to MR. Scatchard analysis demonstrated increased B(max) and K(d) values in the HT of DOCA-treated rats. Occupation of GR by DOCA did not stimulate the ODC activity, in contrast to the few-fold increment effected by the glucocorticoid dexamethasone. Also, administration of RU 486 did not inhibit the salt intake promoted by DOCA, in contrast to ZK 91587 which partly delayed the natriogenic effect of DOCA. It is suggested that brain MR are involved in the natriogenic effect of DOCA, whereas the role of GR is inconclusive. Although GR were occupied by DOCA, this compound did not show agonistic properties regarding induction of ODC nor antagonism of dexamethasone-induced ODC, indicating that DOCA (or its metabolites) rendered inactive GR. |
| title |
Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake |
| title_short |
Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake |
| title_full |
Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake |
| title_fullStr |
Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake |
| title_full_unstemmed |
Further studies in deoxycorticosterone acetate treated rats: Brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake |
| title_sort |
further studies in deoxycorticosterone acetate treated rats: brain content of mineralocorticoid and glucocorticoid receptors and effect of steroid antagonists on salt intake |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v61_n2_p117_Vallee http://hdl.handle.net/20.500.12110/paper_00283835_v61_n2_p117_Vallee |
| _version_ |
1768546710613655552 |