Characterization of a cytosol progesterone receptor in bovine pineal gland
The possible existence of pineal progesterone (P) receptors was assessed in 105,000 g supernatants of bovine pineal glands incubated with3H-P in the presence of 10 μM Cortisol. By charcoal adsorption analysis specific binding (defined as the difference between3H-P binding in the absence and presence...
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1979
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v29_n2_p84_Vacas http://hdl.handle.net/20.500.12110/paper_00283835_v29_n2_p84_Vacas |
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paper:paper_00283835_v29_n2_p84_Vacas2023-06-08T14:54:57Z Characterization of a cytosol progesterone receptor in bovine pineal gland Pineal gland Progesterone Steroid receptors 20alpha dihydroprogesterone 20beta dihydroprogesterone corticosterone estradiol progesterone progesterone receptor radioisotope testosterone animal experiment cattle central nervous system cytosol drug binding drug comparison in vitro study pineal body progesterone h 3 Animal Binding, Competitive Cattle Cytosol Kinetics Pineal Gland Progesterone Receptors, Progesterone The possible existence of pineal progesterone (P) receptors was assessed in 105,000 g supernatants of bovine pineal glands incubated with3H-P in the presence of 10 μM Cortisol. By charcoal adsorption analysis specific binding (defined as the difference between3H-P binding in the absence and presence of 1,000-fold excess of unlabeled P) attained its maximum after 60 min at 37 °C and after 5 h at 0°C. Binding was abolished by heating the cytosol or by pre-incubating it with trypsin. A single population of binding sites with a K<inf>d</inf> = 1.64 nM and binding site concentration = 34 fmol/mg of cytosol protein was detectable by Scatchard analysis. In competition experiments the B<inf>50</inf> progesterone values (nAf) were: P (2), 20β-dihydroproges-terone (12), 20α-dihydroprogesterone (25), estradiol (158), testosterone (675) and corticosterone (> 1,000). After sucrose gradient centrifugation of pineal cytosol incubated with3H-P a discrete peak of radioactivity in the 7-8 S region of the gradient was observed, and disappeared following incubation with excess unlabeled P. These results indicate that P receptors are present in the bovine pineal gland. © 1979 S. Karger AG, Basel. 1979 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v29_n2_p84_Vacas http://hdl.handle.net/20.500.12110/paper_00283835_v29_n2_p84_Vacas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Pineal gland Progesterone Steroid receptors 20alpha dihydroprogesterone 20beta dihydroprogesterone corticosterone estradiol progesterone progesterone receptor radioisotope testosterone animal experiment cattle central nervous system cytosol drug binding drug comparison in vitro study pineal body progesterone h 3 Animal Binding, Competitive Cattle Cytosol Kinetics Pineal Gland Progesterone Receptors, Progesterone |
| spellingShingle |
Pineal gland Progesterone Steroid receptors 20alpha dihydroprogesterone 20beta dihydroprogesterone corticosterone estradiol progesterone progesterone receptor radioisotope testosterone animal experiment cattle central nervous system cytosol drug binding drug comparison in vitro study pineal body progesterone h 3 Animal Binding, Competitive Cattle Cytosol Kinetics Pineal Gland Progesterone Receptors, Progesterone Characterization of a cytosol progesterone receptor in bovine pineal gland |
| topic_facet |
Pineal gland Progesterone Steroid receptors 20alpha dihydroprogesterone 20beta dihydroprogesterone corticosterone estradiol progesterone progesterone receptor radioisotope testosterone animal experiment cattle central nervous system cytosol drug binding drug comparison in vitro study pineal body progesterone h 3 Animal Binding, Competitive Cattle Cytosol Kinetics Pineal Gland Progesterone Receptors, Progesterone |
| description |
The possible existence of pineal progesterone (P) receptors was assessed in 105,000 g supernatants of bovine pineal glands incubated with3H-P in the presence of 10 μM Cortisol. By charcoal adsorption analysis specific binding (defined as the difference between3H-P binding in the absence and presence of 1,000-fold excess of unlabeled P) attained its maximum after 60 min at 37 °C and after 5 h at 0°C. Binding was abolished by heating the cytosol or by pre-incubating it with trypsin. A single population of binding sites with a K<inf>d</inf> = 1.64 nM and binding site concentration = 34 fmol/mg of cytosol protein was detectable by Scatchard analysis. In competition experiments the B<inf>50</inf> progesterone values (nAf) were: P (2), 20β-dihydroproges-terone (12), 20α-dihydroprogesterone (25), estradiol (158), testosterone (675) and corticosterone (> 1,000). After sucrose gradient centrifugation of pineal cytosol incubated with3H-P a discrete peak of radioactivity in the 7-8 S region of the gradient was observed, and disappeared following incubation with excess unlabeled P. These results indicate that P receptors are present in the bovine pineal gland. © 1979 S. Karger AG, Basel. |
| title |
Characterization of a cytosol progesterone receptor in bovine pineal gland |
| title_short |
Characterization of a cytosol progesterone receptor in bovine pineal gland |
| title_full |
Characterization of a cytosol progesterone receptor in bovine pineal gland |
| title_fullStr |
Characterization of a cytosol progesterone receptor in bovine pineal gland |
| title_full_unstemmed |
Characterization of a cytosol progesterone receptor in bovine pineal gland |
| title_sort |
characterization of a cytosol progesterone receptor in bovine pineal gland |
| publishDate |
1979 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v29_n2_p84_Vacas http://hdl.handle.net/20.500.12110/paper_00283835_v29_n2_p84_Vacas |
| _version_ |
1768544444823371776 |