A methyl transferase links the circadian clock to the regulation of alternative splicing

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Circadian rhythms allow organisms to time biological processes to the most appropriate phases of the dayg-night cycle. Post-transcriptional regulation is emerging as an important component of circadian networks, but the molecular mechanisms linking the circadian clock to the control of RNA processin...

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Autores principales:	Sanchez, Sabrina Elena, Petrillo, Ezequiel, Beckwith, Esteban Javier, Godoy Herz, Micaela Amalia, Depetris Chauvin, Ana, Cerdán, Pablo Diego, Ceriani, María Fernanda, Kornblihtt, Alberto Rodolfo, Yanovsky, Marcelo Javier
Publicado:	2010
Materias:	arginine derivative histone methyl group methyltransferase protein arginine methyltransferase protein arginine methyltransferase 5 protein Sm pseudo response regulator 9 regulator protein RNA unclassified drug circadian rhythm enzyme activity fly gene expression genetic analysis genome physiological response alternative RNA splicing Arabidopsis article controlled study Drosophila melanogaster gene disruption gene mutation gene overexpression locomotion molecular clock molecular recognition nonhuman oscillation phenotype priority journal protein expression Alternative Splicing Animals Arabidopsis Proteins Base Sequence Circadian Clocks Circadian Rhythm Darkness Drosophila Proteins Gene Expression Profiling Gene Expression Regulation, Plant Light Methylation Mutation Period Circadian Proteins Phenotype Protein Methyltransferases Protein-Arginine N-Methyltransferases RNA Precursors RNA Splice Sites RNA, Messenger Spliceosomes Transcription Factors Arabidopsis thaliana
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00280836_v468_n7320_p112_Sanchez http://hdl.handle.net/20.500.12110/paper_00280836_v468_n7320_p112_Sanchez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00280836_v468_n7320_p112_Sanchez
record_format	dspace
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	arginine derivative histone methyl group methyltransferase protein arginine methyltransferase protein arginine methyltransferase 5 protein Sm pseudo response regulator 9 regulator protein RNA unclassified drug circadian rhythm enzyme activity fly gene expression genetic analysis genome physiological response alternative RNA splicing Arabidopsis article circadian rhythm controlled study Drosophila melanogaster gene disruption gene mutation gene overexpression locomotion molecular clock molecular recognition nonhuman oscillation phenotype priority journal protein expression Alternative Splicing Animals Arabidopsis Arabidopsis Proteins Base Sequence Circadian Clocks Circadian Rhythm Darkness Drosophila melanogaster Drosophila Proteins Gene Expression Profiling Gene Expression Regulation, Plant Light Methylation Mutation Period Circadian Proteins Phenotype Protein Methyltransferases Protein-Arginine N-Methyltransferases RNA Precursors RNA Splice Sites RNA, Messenger Spliceosomes Transcription Factors Arabidopsis thaliana Drosophila melanogaster
spellingShingle	arginine derivative histone methyl group methyltransferase protein arginine methyltransferase protein arginine methyltransferase 5 protein Sm pseudo response regulator 9 regulator protein RNA unclassified drug circadian rhythm enzyme activity fly gene expression genetic analysis genome physiological response alternative RNA splicing Arabidopsis article circadian rhythm controlled study Drosophila melanogaster gene disruption gene mutation gene overexpression locomotion molecular clock molecular recognition nonhuman oscillation phenotype priority journal protein expression Alternative Splicing Animals Arabidopsis Arabidopsis Proteins Base Sequence Circadian Clocks Circadian Rhythm Darkness Drosophila melanogaster Drosophila Proteins Gene Expression Profiling Gene Expression Regulation, Plant Light Methylation Mutation Period Circadian Proteins Phenotype Protein Methyltransferases Protein-Arginine N-Methyltransferases RNA Precursors RNA Splice Sites RNA, Messenger Spliceosomes Transcription Factors Arabidopsis thaliana Drosophila melanogaster Sanchez, Sabrina Elena Petrillo, Ezequiel Beckwith, Esteban Javier Godoy Herz, Micaela Amalia Depetris Chauvin, Ana Cerdán, Pablo Diego Ceriani, María Fernanda Kornblihtt, Alberto Rodolfo Yanovsky, Marcelo Javier A methyl transferase links the circadian clock to the regulation of alternative splicing
topic_facet	arginine derivative histone methyl group methyltransferase protein arginine methyltransferase protein arginine methyltransferase 5 protein Sm pseudo response regulator 9 regulator protein RNA unclassified drug circadian rhythm enzyme activity fly gene expression genetic analysis genome physiological response alternative RNA splicing Arabidopsis article circadian rhythm controlled study Drosophila melanogaster gene disruption gene mutation gene overexpression locomotion molecular clock molecular recognition nonhuman oscillation phenotype priority journal protein expression Alternative Splicing Animals Arabidopsis Arabidopsis Proteins Base Sequence Circadian Clocks Circadian Rhythm Darkness Drosophila melanogaster Drosophila Proteins Gene Expression Profiling Gene Expression Regulation, Plant Light Methylation Mutation Period Circadian Proteins Phenotype Protein Methyltransferases Protein-Arginine N-Methyltransferases RNA Precursors RNA Splice Sites RNA, Messenger Spliceosomes Transcription Factors Arabidopsis thaliana Drosophila melanogaster
description	Circadian rhythms allow organisms to time biological processes to the most appropriate phases of the dayg-night cycle. Post-transcriptional regulation is emerging as an important component of circadian networks, but the molecular mechanisms linking the circadian clock to the control of RNA processing are largely unknown. Here we show that PROTEIN ARGININE METHYL TRANSFERASE 5 (PRMT5), which transfers methyl groups to arginine residues present in histones and Sm spliceosomal proteins, links the circadian clock to the control of alternative splicing in plants. Mutations in PRMT5 impair several circadian rhythms in Arabidopsis thaliana and this phenotype is caused, at least in part, by a strong alteration in alternative splicing of the core-clock gene PSEUDO RESPONSE REGULATOR 9 (PRR9). Furthermore, genome-wide studies show that PRMT5 contributes to the regulation of many pre-messenger-RNA splicing events, probably by modulating 5ĝ€2-splice-site recognition. PRMT5 expression shows daily and circadian oscillations, and this contributes to the mediation of the circadian regulation of expression and alternative splicing of a subset of genes. Circadian rhythms in locomotor activity are also disrupted in dart5-1, a mutant affected in the Drosophila melanogaster PRMT5 homologue, and this is associated with alterations in splicing of the core-clock gene period and several clock-associated genes. Our results demonstrate a key role for PRMT5 in the regulation of alternative splicing and indicate that the interplay between the circadian clock and the regulation of alternative splicing by PRMT5 constitutes a common mechanism that helps organisms to synchronize physiological processes with daily changes in environmental conditions. © 2010 Macmillan Publishers Limited. All rights reserved.
author	Sanchez, Sabrina Elena Petrillo, Ezequiel Beckwith, Esteban Javier Godoy Herz, Micaela Amalia Depetris Chauvin, Ana Cerdán, Pablo Diego Ceriani, María Fernanda Kornblihtt, Alberto Rodolfo Yanovsky, Marcelo Javier
author_facet	Sanchez, Sabrina Elena Petrillo, Ezequiel Beckwith, Esteban Javier Godoy Herz, Micaela Amalia Depetris Chauvin, Ana Cerdán, Pablo Diego Ceriani, María Fernanda Kornblihtt, Alberto Rodolfo Yanovsky, Marcelo Javier
author_sort	Sanchez, Sabrina Elena
title	A methyl transferase links the circadian clock to the regulation of alternative splicing
title_short	A methyl transferase links the circadian clock to the regulation of alternative splicing
title_full	A methyl transferase links the circadian clock to the regulation of alternative splicing
title_fullStr	A methyl transferase links the circadian clock to the regulation of alternative splicing
title_full_unstemmed	A methyl transferase links the circadian clock to the regulation of alternative splicing
title_sort	methyl transferase links the circadian clock to the regulation of alternative splicing
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00280836_v468_n7320_p112_Sanchez http://hdl.handle.net/20.500.12110/paper_00280836_v468_n7320_p112_Sanchez
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spelling	paper:paper_00280836_v468_n7320_p112_Sanchez2023-06-08T14:54:45Z A methyl transferase links the circadian clock to the regulation of alternative splicing Sanchez, Sabrina Elena Petrillo, Ezequiel Beckwith, Esteban Javier Godoy Herz, Micaela Amalia Depetris Chauvin, Ana Cerdán, Pablo Diego Ceriani, María Fernanda Kornblihtt, Alberto Rodolfo Yanovsky, Marcelo Javier arginine derivative histone methyl group methyltransferase protein arginine methyltransferase protein arginine methyltransferase 5 protein Sm pseudo response regulator 9 regulator protein RNA unclassified drug circadian rhythm enzyme activity fly gene expression genetic analysis genome physiological response alternative RNA splicing Arabidopsis article circadian rhythm controlled study Drosophila melanogaster gene disruption gene mutation gene overexpression locomotion molecular clock molecular recognition nonhuman oscillation phenotype priority journal protein expression Alternative Splicing Animals Arabidopsis Arabidopsis Proteins Base Sequence Circadian Clocks Circadian Rhythm Darkness Drosophila melanogaster Drosophila Proteins Gene Expression Profiling Gene Expression Regulation, Plant Light Methylation Mutation Period Circadian Proteins Phenotype Protein Methyltransferases Protein-Arginine N-Methyltransferases RNA Precursors RNA Splice Sites RNA, Messenger Spliceosomes Transcription Factors Arabidopsis thaliana Drosophila melanogaster Circadian rhythms allow organisms to time biological processes to the most appropriate phases of the dayg-night cycle. Post-transcriptional regulation is emerging as an important component of circadian networks, but the molecular mechanisms linking the circadian clock to the control of RNA processing are largely unknown. Here we show that PROTEIN ARGININE METHYL TRANSFERASE 5 (PRMT5), which transfers methyl groups to arginine residues present in histones and Sm spliceosomal proteins, links the circadian clock to the control of alternative splicing in plants. Mutations in PRMT5 impair several circadian rhythms in Arabidopsis thaliana and this phenotype is caused, at least in part, by a strong alteration in alternative splicing of the core-clock gene PSEUDO RESPONSE REGULATOR 9 (PRR9). Furthermore, genome-wide studies show that PRMT5 contributes to the regulation of many pre-messenger-RNA splicing events, probably by modulating 5ĝ€2-splice-site recognition. PRMT5 expression shows daily and circadian oscillations, and this contributes to the mediation of the circadian regulation of expression and alternative splicing of a subset of genes. Circadian rhythms in locomotor activity are also disrupted in dart5-1, a mutant affected in the Drosophila melanogaster PRMT5 homologue, and this is associated with alterations in splicing of the core-clock gene period and several clock-associated genes. Our results demonstrate a key role for PRMT5 in the regulation of alternative splicing and indicate that the interplay between the circadian clock and the regulation of alternative splicing by PRMT5 constitutes a common mechanism that helps organisms to synchronize physiological processes with daily changes in environmental conditions. © 2010 Macmillan Publishers Limited. All rights reserved. Fil:Sanchez, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Petrillo, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Beckwith, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godoy Herz, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Depetris-Chauvin, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerdán, P.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceriani, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Yanovsky, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00280836_v468_n7320_p112_Sanchez http://hdl.handle.net/20.500.12110/paper_00280836_v468_n7320_p112_Sanchez

A methyl transferase links the circadian clock to the regulation of alternative splicing

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