α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
We report the cloning and characterization of rat α10, a previously unidentified member of the nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by the α10 nAChR subunit gene is most similar to the rat α9 nAChR, and both α9 and α10 subunit genes are transcribed in adu...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v98_n6_p3501_Elgoyhen http://hdl.handle.net/20.500.12110/paper_00278424_v98_n6_p3501_Elgoyhen |
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paper:paper_00278424_v98_n6_p3501_Elgoyhen2023-06-08T14:54:38Z α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells Katz, Eleonora 1,1 dimethyl 4 phenylpiperazinium acetylcholine alpha bungarotoxin atropine bicuculline carbachol cholinergic receptor cholinergic receptor blocking agent cholinergic receptor stimulating agent muscarine nicotine nicotinic agent nicotinic receptor nicotinic receptor blocking agent oxotremorine receptor subunit strychnine tropisetron tubocurarine chloride nicotinic receptor animal cell article cholinergic receptor blocking cochlea dose response drug receptor binding extracellular calcium hair cell human molecular cloning multigene family nonhuman nucleotide sequence oocyte priority journal rat receptor down regulation vestibule Xenopus laevis amino acid sequence animal cochlea cytology female gene expression genetics metabolism molecular genetics mouse nucleotide sequence physiology vestibule Animalia Mammalia Strychnos toxifera Vertebrata Xenopus laevis Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cochlea Female Gene Expression Hair Cells Hair Cells, Vestibular Humans Mice Molecular Sequence Data Rats Receptors, Nicotinic Vestibule Xenopus laevis We report the cloning and characterization of rat α10, a previously unidentified member of the nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by the α10 nAChR subunit gene is most similar to the rat α9 nAChR, and both α9 and α10 subunit genes are transcribed in adult rat mechanosensory hair cells. Injection of Xenopus laevis oocytes with α10 cRNA alone or in pairwise combinations with either α2-α6 or β2-β4 subunit cRNAs yielded no detectable ACh-gated currents. However, coinjection of α9 and α10 cRNAs resulted in the appearance of an unusual nAChR subtype. Compared with homomeric α9 channels, the α9α10 nAChR subtype displays faster and more extensive agonist-mediated desensitization, a distinct current-voltage relationship, and a biphasic response to changes in extracellular Ca2+ ions. The pharmacological profiles of homomeric α9 and heteromeric α9α10 nAChRs are essentially indistinguishable and closely resemble those reported for endogenous cholinergic eceptors found in vertebrate hair cells. Our data suggest that efferent modulation of hair cell function occurs, at least in part, through heteromeric nAChRs assembled from both α9 and α10 subunits. Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v98_n6_p3501_Elgoyhen http://hdl.handle.net/20.500.12110/paper_00278424_v98_n6_p3501_Elgoyhen |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
1,1 dimethyl 4 phenylpiperazinium acetylcholine alpha bungarotoxin atropine bicuculline carbachol cholinergic receptor cholinergic receptor blocking agent cholinergic receptor stimulating agent muscarine nicotine nicotinic agent nicotinic receptor nicotinic receptor blocking agent oxotremorine receptor subunit strychnine tropisetron tubocurarine chloride nicotinic receptor animal cell article cholinergic receptor blocking cochlea dose response drug receptor binding extracellular calcium hair cell human molecular cloning multigene family nonhuman nucleotide sequence oocyte priority journal rat receptor down regulation vestibule Xenopus laevis amino acid sequence animal cochlea cytology female gene expression genetics metabolism molecular genetics mouse nucleotide sequence physiology vestibule Animalia Mammalia Strychnos toxifera Vertebrata Xenopus laevis Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cochlea Female Gene Expression Hair Cells Hair Cells, Vestibular Humans Mice Molecular Sequence Data Rats Receptors, Nicotinic Vestibule Xenopus laevis |
| spellingShingle |
1,1 dimethyl 4 phenylpiperazinium acetylcholine alpha bungarotoxin atropine bicuculline carbachol cholinergic receptor cholinergic receptor blocking agent cholinergic receptor stimulating agent muscarine nicotine nicotinic agent nicotinic receptor nicotinic receptor blocking agent oxotremorine receptor subunit strychnine tropisetron tubocurarine chloride nicotinic receptor animal cell article cholinergic receptor blocking cochlea dose response drug receptor binding extracellular calcium hair cell human molecular cloning multigene family nonhuman nucleotide sequence oocyte priority journal rat receptor down regulation vestibule Xenopus laevis amino acid sequence animal cochlea cytology female gene expression genetics metabolism molecular genetics mouse nucleotide sequence physiology vestibule Animalia Mammalia Strychnos toxifera Vertebrata Xenopus laevis Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cochlea Female Gene Expression Hair Cells Hair Cells, Vestibular Humans Mice Molecular Sequence Data Rats Receptors, Nicotinic Vestibule Xenopus laevis Katz, Eleonora α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells |
| topic_facet |
1,1 dimethyl 4 phenylpiperazinium acetylcholine alpha bungarotoxin atropine bicuculline carbachol cholinergic receptor cholinergic receptor blocking agent cholinergic receptor stimulating agent muscarine nicotine nicotinic agent nicotinic receptor nicotinic receptor blocking agent oxotremorine receptor subunit strychnine tropisetron tubocurarine chloride nicotinic receptor animal cell article cholinergic receptor blocking cochlea dose response drug receptor binding extracellular calcium hair cell human molecular cloning multigene family nonhuman nucleotide sequence oocyte priority journal rat receptor down regulation vestibule Xenopus laevis amino acid sequence animal cochlea cytology female gene expression genetics metabolism molecular genetics mouse nucleotide sequence physiology vestibule Animalia Mammalia Strychnos toxifera Vertebrata Xenopus laevis Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cochlea Female Gene Expression Hair Cells Hair Cells, Vestibular Humans Mice Molecular Sequence Data Rats Receptors, Nicotinic Vestibule Xenopus laevis |
| description |
We report the cloning and characterization of rat α10, a previously unidentified member of the nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by the α10 nAChR subunit gene is most similar to the rat α9 nAChR, and both α9 and α10 subunit genes are transcribed in adult rat mechanosensory hair cells. Injection of Xenopus laevis oocytes with α10 cRNA alone or in pairwise combinations with either α2-α6 or β2-β4 subunit cRNAs yielded no detectable ACh-gated currents. However, coinjection of α9 and α10 cRNAs resulted in the appearance of an unusual nAChR subtype. Compared with homomeric α9 channels, the α9α10 nAChR subtype displays faster and more extensive agonist-mediated desensitization, a distinct current-voltage relationship, and a biphasic response to changes in extracellular Ca2+ ions. The pharmacological profiles of homomeric α9 and heteromeric α9α10 nAChRs are essentially indistinguishable and closely resemble those reported for endogenous cholinergic eceptors found in vertebrate hair cells. Our data suggest that efferent modulation of hair cell function occurs, at least in part, through heteromeric nAChRs assembled from both α9 and α10 subunits. |
| author |
Katz, Eleonora |
| author_facet |
Katz, Eleonora |
| author_sort |
Katz, Eleonora |
| title |
α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells |
| title_short |
α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells |
| title_full |
α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells |
| title_fullStr |
α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells |
| title_full_unstemmed |
α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells |
| title_sort |
α10: a determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v98_n6_p3501_Elgoyhen http://hdl.handle.net/20.500.12110/paper_00278424_v98_n6_p3501_Elgoyhen |
| work_keys_str_mv |
AT katzeleonora a10adeterminantofnicotiniccholinergicreceptorfunctioninmammalianvestibularandcochlearmechanosensoryhaircells |
| _version_ |
1768541835207114752 |