The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
We present analysis, by cryo-electron microscopy and single-particle reconstruction, of the structure of the 80S ribosome from Trypanosoma cruzi, the kinetoplastid protozoan pathogen that causes Chagas disease. The density map of the T. cruzi 80S ribosome shows the phylogenetically conserved eukaryo...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v102_n29_p10206_Gao http://hdl.handle.net/20.500.12110/paper_00278424_v102_n29_p10206_Gao |
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paper:paper_00278424_v102_n29_p10206_Gao2023-06-08T14:54:21Z The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components Eukaryote Initiation Translation messenger RNA ribosome RNA 80s ribosome subunit article Chagas disease cryoelectron microscopy eukaryote genetic conservation nonhuman priority journal ribosome subunit RNA structure RNA translation structure analysis translation initiation Trypanosoma cruzi Animals Base Pairing Cryoelectron Microscopy Image Processing, Computer-Assisted Models, Molecular Ribosomes RNA, Ribosomal Trypanosoma cruzi Eukaryota Kinetoplastida Protozoa Trypanosoma cruzi We present analysis, by cryo-electron microscopy and single-particle reconstruction, of the structure of the 80S ribosome from Trypanosoma cruzi, the kinetoplastid protozoan pathogen that causes Chagas disease. The density map of the T. cruzi 80S ribosome shows the phylogenetically conserved eukaryotic rRNA core structure, together with distinctive structural features in both the small and large subunits. Remarkably, a previously undescribed helical structure appears in the small subunit in the vicinity of the mRNA exit channel. We propose that this rRNA structure likely participates in the recruitment of ribosome onto the 5′ end of mRNA, in facilitating and modulating the initiation of translation that is unique to the trypanosomes. © 2005 by The National Academy of Sciences of the USA. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v102_n29_p10206_Gao http://hdl.handle.net/20.500.12110/paper_00278424_v102_n29_p10206_Gao |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Eukaryote Initiation Translation messenger RNA ribosome RNA 80s ribosome subunit article Chagas disease cryoelectron microscopy eukaryote genetic conservation nonhuman priority journal ribosome subunit RNA structure RNA translation structure analysis translation initiation Trypanosoma cruzi Animals Base Pairing Cryoelectron Microscopy Image Processing, Computer-Assisted Models, Molecular Ribosomes RNA, Ribosomal Trypanosoma cruzi Eukaryota Kinetoplastida Protozoa Trypanosoma cruzi |
| spellingShingle |
Eukaryote Initiation Translation messenger RNA ribosome RNA 80s ribosome subunit article Chagas disease cryoelectron microscopy eukaryote genetic conservation nonhuman priority journal ribosome subunit RNA structure RNA translation structure analysis translation initiation Trypanosoma cruzi Animals Base Pairing Cryoelectron Microscopy Image Processing, Computer-Assisted Models, Molecular Ribosomes RNA, Ribosomal Trypanosoma cruzi Eukaryota Kinetoplastida Protozoa Trypanosoma cruzi The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components |
| topic_facet |
Eukaryote Initiation Translation messenger RNA ribosome RNA 80s ribosome subunit article Chagas disease cryoelectron microscopy eukaryote genetic conservation nonhuman priority journal ribosome subunit RNA structure RNA translation structure analysis translation initiation Trypanosoma cruzi Animals Base Pairing Cryoelectron Microscopy Image Processing, Computer-Assisted Models, Molecular Ribosomes RNA, Ribosomal Trypanosoma cruzi Eukaryota Kinetoplastida Protozoa Trypanosoma cruzi |
| description |
We present analysis, by cryo-electron microscopy and single-particle reconstruction, of the structure of the 80S ribosome from Trypanosoma cruzi, the kinetoplastid protozoan pathogen that causes Chagas disease. The density map of the T. cruzi 80S ribosome shows the phylogenetically conserved eukaryotic rRNA core structure, together with distinctive structural features in both the small and large subunits. Remarkably, a previously undescribed helical structure appears in the small subunit in the vicinity of the mRNA exit channel. We propose that this rRNA structure likely participates in the recruitment of ribosome onto the 5′ end of mRNA, in facilitating and modulating the initiation of translation that is unique to the trypanosomes. © 2005 by The National Academy of Sciences of the USA. |
| title |
The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components |
| title_short |
The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components |
| title_full |
The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components |
| title_fullStr |
The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components |
| title_full_unstemmed |
The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components |
| title_sort |
structure of the 80s ribosome from trypanosoma cruzi reveals unique rrna components |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v102_n29_p10206_Gao http://hdl.handle.net/20.500.12110/paper_00278424_v102_n29_p10206_Gao |
| _version_ |
1768543598326841344 |