Corticotropin-releasing hormone activates ERK1/2 MAPK in specific brain areas
Corticotropin-releasing hormone (CRH) coordinates hormonal and behavioral responses to stress. The mitogen-activated protein kinase extracellular signal-related kinase 1/2 (ERK1/2) mediates several functions in different forebrain structures and recently has been implicated in CRH signaling in cultu...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v102_n17_p6183_Refojo http://hdl.handle.net/20.500.12110/paper_00278424_v102_n17_p6183_Refojo |
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paper:paper_00278424_v102_n17_p6183_Refojo2023-06-08T14:54:20Z Corticotropin-releasing hormone activates ERK1/2 MAPK in specific brain areas Corticotropin-releasing hormone receptor 1 conditional knockout Extracellular signal-regulated kinase 1/2 Mitogen-activated protein kinase corticotropin releasing factor corticotropin releasing factor receptor 1 corticotropin releasing factor receptor 2 mitogen activated protein kinase 1 mitogen activated protein kinase 3 adaptation amygdaloid nucleus animal experiment animal tissue article brain brain region confocal microscopy controlled study crhr1 gene enzyme activation forebrain gene gene inactivation hippocampus hormone action hypothalamus immunohistochemistry knockout mouse limbic cortex male mouse nonhuman priority journal protein expression stress Animals Brain Corticotropin-Releasing Hormone Enzyme Activation Hypothalamus Male Mice Mice, Transgenic Microscopy, Confocal Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Organ Specificity Prosencephalon Corticotropin-releasing hormone (CRH) coordinates hormonal and behavioral responses to stress. The mitogen-activated protein kinase extracellular signal-related kinase 1/2 (ERK1/2) mediates several functions in different forebrain structures and recently has been implicated in CRH signaling in cultured cells. To study in vivo CRH-mediated activation of central ERK1/2, we investigated the expression pattern of the phosphorylated ERK1/2 (p-ERK1/2) in the mouse brain after intracerebroventricular CRH injections. As shown by immunohistochemistry and confocal microscopy analysis, CRH administration increased p-ERK1/2 levels specifically in the CA3 and CA1 hippocampal subfields and basolateral complex of the amygdala, both structures related to external environmental information processing and behavioral aspects of stress. Other regions such as hypothalamic nuclei and the central nucleus of the amygdala, also related to central CRH system but involved in the processing of the ascending visceral information and neuroendocrine-autonomic response to stress, did not show CRH-mediated ERK1/2 activation. To dissect the involvement of CRH receptor 1 (CRHR1) and CRHR2, we used conditional knockout mice in which Crhr1 is inactivated in the anterior forebrain and limbic structures. The conditional genetic ablation of Crhr1 inhibited the p-ERK1/2 increase, underlining the involvement of CRHR1 in the CRH-mediated activation. These findings underscore the fact that CRH activates p-ERK1/2 through CRHR1 only in selected brain regions, pointing to a specific role of this pathway in mediating behavioral adaptation to stress. © 2005 by The National Academy of Sciences of the USA. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v102_n17_p6183_Refojo http://hdl.handle.net/20.500.12110/paper_00278424_v102_n17_p6183_Refojo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Corticotropin-releasing hormone receptor 1 conditional knockout Extracellular signal-regulated kinase 1/2 Mitogen-activated protein kinase corticotropin releasing factor corticotropin releasing factor receptor 1 corticotropin releasing factor receptor 2 mitogen activated protein kinase 1 mitogen activated protein kinase 3 adaptation amygdaloid nucleus animal experiment animal tissue article brain brain region confocal microscopy controlled study crhr1 gene enzyme activation forebrain gene gene inactivation hippocampus hormone action hypothalamus immunohistochemistry knockout mouse limbic cortex male mouse nonhuman priority journal protein expression stress Animals Brain Corticotropin-Releasing Hormone Enzyme Activation Hypothalamus Male Mice Mice, Transgenic Microscopy, Confocal Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Organ Specificity Prosencephalon |
| spellingShingle |
Corticotropin-releasing hormone receptor 1 conditional knockout Extracellular signal-regulated kinase 1/2 Mitogen-activated protein kinase corticotropin releasing factor corticotropin releasing factor receptor 1 corticotropin releasing factor receptor 2 mitogen activated protein kinase 1 mitogen activated protein kinase 3 adaptation amygdaloid nucleus animal experiment animal tissue article brain brain region confocal microscopy controlled study crhr1 gene enzyme activation forebrain gene gene inactivation hippocampus hormone action hypothalamus immunohistochemistry knockout mouse limbic cortex male mouse nonhuman priority journal protein expression stress Animals Brain Corticotropin-Releasing Hormone Enzyme Activation Hypothalamus Male Mice Mice, Transgenic Microscopy, Confocal Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Organ Specificity Prosencephalon Corticotropin-releasing hormone activates ERK1/2 MAPK in specific brain areas |
| topic_facet |
Corticotropin-releasing hormone receptor 1 conditional knockout Extracellular signal-regulated kinase 1/2 Mitogen-activated protein kinase corticotropin releasing factor corticotropin releasing factor receptor 1 corticotropin releasing factor receptor 2 mitogen activated protein kinase 1 mitogen activated protein kinase 3 adaptation amygdaloid nucleus animal experiment animal tissue article brain brain region confocal microscopy controlled study crhr1 gene enzyme activation forebrain gene gene inactivation hippocampus hormone action hypothalamus immunohistochemistry knockout mouse limbic cortex male mouse nonhuman priority journal protein expression stress Animals Brain Corticotropin-Releasing Hormone Enzyme Activation Hypothalamus Male Mice Mice, Transgenic Microscopy, Confocal Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Organ Specificity Prosencephalon |
| description |
Corticotropin-releasing hormone (CRH) coordinates hormonal and behavioral responses to stress. The mitogen-activated protein kinase extracellular signal-related kinase 1/2 (ERK1/2) mediates several functions in different forebrain structures and recently has been implicated in CRH signaling in cultured cells. To study in vivo CRH-mediated activation of central ERK1/2, we investigated the expression pattern of the phosphorylated ERK1/2 (p-ERK1/2) in the mouse brain after intracerebroventricular CRH injections. As shown by immunohistochemistry and confocal microscopy analysis, CRH administration increased p-ERK1/2 levels specifically in the CA3 and CA1 hippocampal subfields and basolateral complex of the amygdala, both structures related to external environmental information processing and behavioral aspects of stress. Other regions such as hypothalamic nuclei and the central nucleus of the amygdala, also related to central CRH system but involved in the processing of the ascending visceral information and neuroendocrine-autonomic response to stress, did not show CRH-mediated ERK1/2 activation. To dissect the involvement of CRH receptor 1 (CRHR1) and CRHR2, we used conditional knockout mice in which Crhr1 is inactivated in the anterior forebrain and limbic structures. The conditional genetic ablation of Crhr1 inhibited the p-ERK1/2 increase, underlining the involvement of CRHR1 in the CRH-mediated activation. These findings underscore the fact that CRH activates p-ERK1/2 through CRHR1 only in selected brain regions, pointing to a specific role of this pathway in mediating behavioral adaptation to stress. © 2005 by The National Academy of Sciences of the USA. |
| title |
Corticotropin-releasing hormone activates ERK1/2 MAPK in specific brain areas |
| title_short |
Corticotropin-releasing hormone activates ERK1/2 MAPK in specific brain areas |
| title_full |
Corticotropin-releasing hormone activates ERK1/2 MAPK in specific brain areas |
| title_fullStr |
Corticotropin-releasing hormone activates ERK1/2 MAPK in specific brain areas |
| title_full_unstemmed |
Corticotropin-releasing hormone activates ERK1/2 MAPK in specific brain areas |
| title_sort |
corticotropin-releasing hormone activates erk1/2 mapk in specific brain areas |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v102_n17_p6183_Refojo http://hdl.handle.net/20.500.12110/paper_00278424_v102_n17_p6183_Refojo |
| _version_ |
1768541786987298816 |