Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels

Transmission at the mouse neuromuscular junction normally relies on P/Q-type channels, but became jointly dependent on both N-and R-type Ca2+ channels when the P/Q-type channel α1A subunit was deleted. R-type channels lay close to Ca2+ sensors for exocytosis and IK(Ca) channel activation, like the P...

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Autor principal:	Uchitel, Osvaldo Daniel
Publicado:	2003
Materias:	α1A knockout Ca2+-activated potassium channel Neuromuscular junction Paired-pulse facilitation SNX-482 calcium channel calcium ion cholinergic receptor magnesium ion action potential animal cell article channel gating exocytosis mouse nerve ending neuromuscular synapse neurotransmission neurotransmitter release nonhuman priority journal 4-Aminopyridine Animals Calcium Channels, N-Type Calcium Channels, P-Type Calcium Channels, Q-Type Calcium Channels, R-Type Mice Mice, Knockout Models, Neurological Motor Endplate Neuronal Plasticity Neurotransmitter Agents Synaptic Transmission Animalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v100_n6_p3491_Urbano http://hdl.handle.net/20.500.12110/paper_00278424_v100_n6_p3491_Urbano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00278424_v100_n6_p3491_Urbano
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spelling	paper:paper_00278424_v100_n6_p3491_Urbano2023-06-08T14:54:19Z Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels Uchitel, Osvaldo Daniel α1A knockout Ca2+-activated potassium channel Neuromuscular junction Paired-pulse facilitation SNX-482 calcium channel calcium ion cholinergic receptor magnesium ion action potential animal cell article channel gating exocytosis mouse nerve ending neuromuscular synapse neurotransmission neurotransmitter release nonhuman priority journal 4-Aminopyridine Animals Calcium Channels, N-Type Calcium Channels, P-Type Calcium Channels, Q-Type Calcium Channels, R-Type Mice Mice, Knockout Models, Neurological Motor Endplate Neuronal Plasticity Neurotransmitter Agents Synaptic Transmission Animalia Transmission at the mouse neuromuscular junction normally relies on P/Q-type channels, but became jointly dependent on both N-and R-type Ca2+ channels when the P/Q-type channel α1A subunit was deleted. R-type channels lay close to Ca2+ sensors for exocytosis and IK(Ca) channel activation, like the P/Q-type channels they replaced. In contrast, N-type channels were less well localized, but abundant enough to influence secretion strongly, particularly when action potentials were prolonged. Our data suggested that active zone structures may select among multiple Ca2+ channels in the hierarchy P/Q>R>N. The α1A-/- neuromuscular junction displayed several other differences from wild-type: lowered quantal content but greater ability to withstand reductions in the Ca2+/Mg2+ ratio, and little or no paired-pulse facilitation, the latter findings possibly reflecting compensatory mechanisms at individual release sites. Changes in presynaptic function were also associated with a significant reduction in the size of postsynaptic acetylcholine receptor clusters. Fil:Uchitel, O.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v100_n6_p3491_Urbano http://hdl.handle.net/20.500.12110/paper_00278424_v100_n6_p3491_Urbano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	α1A knockout Ca2+-activated potassium channel Neuromuscular junction Paired-pulse facilitation SNX-482 calcium channel calcium ion cholinergic receptor magnesium ion action potential animal cell article channel gating exocytosis mouse nerve ending neuromuscular synapse neurotransmission neurotransmitter release nonhuman priority journal 4-Aminopyridine Animals Calcium Channels, N-Type Calcium Channels, P-Type Calcium Channels, Q-Type Calcium Channels, R-Type Mice Mice, Knockout Models, Neurological Motor Endplate Neuronal Plasticity Neurotransmitter Agents Synaptic Transmission Animalia
spellingShingle	α1A knockout Ca2+-activated potassium channel Neuromuscular junction Paired-pulse facilitation SNX-482 calcium channel calcium ion cholinergic receptor magnesium ion action potential animal cell article channel gating exocytosis mouse nerve ending neuromuscular synapse neurotransmission neurotransmitter release nonhuman priority journal 4-Aminopyridine Animals Calcium Channels, N-Type Calcium Channels, P-Type Calcium Channels, Q-Type Calcium Channels, R-Type Mice Mice, Knockout Models, Neurological Motor Endplate Neuronal Plasticity Neurotransmitter Agents Synaptic Transmission Animalia Uchitel, Osvaldo Daniel Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels
topic_facet	α1A knockout Ca2+-activated potassium channel Neuromuscular junction Paired-pulse facilitation SNX-482 calcium channel calcium ion cholinergic receptor magnesium ion action potential animal cell article channel gating exocytosis mouse nerve ending neuromuscular synapse neurotransmission neurotransmitter release nonhuman priority journal 4-Aminopyridine Animals Calcium Channels, N-Type Calcium Channels, P-Type Calcium Channels, Q-Type Calcium Channels, R-Type Mice Mice, Knockout Models, Neurological Motor Endplate Neuronal Plasticity Neurotransmitter Agents Synaptic Transmission Animalia
description	Transmission at the mouse neuromuscular junction normally relies on P/Q-type channels, but became jointly dependent on both N-and R-type Ca2+ channels when the P/Q-type channel α1A subunit was deleted. R-type channels lay close to Ca2+ sensors for exocytosis and IK(Ca) channel activation, like the P/Q-type channels they replaced. In contrast, N-type channels were less well localized, but abundant enough to influence secretion strongly, particularly when action potentials were prolonged. Our data suggested that active zone structures may select among multiple Ca2+ channels in the hierarchy P/Q>R>N. The α1A-/- neuromuscular junction displayed several other differences from wild-type: lowered quantal content but greater ability to withstand reductions in the Ca2+/Mg2+ ratio, and little or no paired-pulse facilitation, the latter findings possibly reflecting compensatory mechanisms at individual release sites. Changes in presynaptic function were also associated with a significant reduction in the size of postsynaptic acetylcholine receptor clusters.
author	Uchitel, Osvaldo Daniel
author_facet	Uchitel, Osvaldo Daniel
author_sort	Uchitel, Osvaldo Daniel
title	Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels
title_short	Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels
title_full	Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels
title_fullStr	Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels
title_full_unstemmed	Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels
title_sort	altered properties of quantal neurotransmitter release at endplates of mice lacking p/q-type ca2+ channels
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v100_n6_p3491_Urbano http://hdl.handle.net/20.500.12110/paper_00278424_v100_n6_p3491_Urbano
work_keys_str_mv	AT uchitelosvaldodaniel alteredpropertiesofquantalneurotransmitterreleaseatendplatesofmicelackingpqtypeca2channels
_version_	1768543122045796352

Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels

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