Molecular phylogenetic and zoospore ultrastructural analyses of Chytridium olla establish the limits of a monophyletic Chytridiales
Chytridium olla A. Braun, the first described chytrid and an obligate algal parasite, is the type for the genus and thus the foundation of family Chytridiaceae, order Chytridiales, class Chytridiomycetes and phylum Chytridiomycota. Chytridium olla was isolated in coculture with its host, Oedogonium...
Guardado en:
| Publicado: |
2011
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v103_n1_p118_Velez http://hdl.handle.net/20.500.12110/paper_00275514_v103_n1_p118_Velez |
| Aporte de: |
| id |
paper:paper_00275514_v103_n1_p118_Velez |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00275514_v103_n1_p118_Velez2023-06-08T14:54:11Z Molecular phylogenetic and zoospore ultrastructural analyses of Chytridium olla establish the limits of a monophyletic Chytridiales 18S rDNA 28S rDNA Chytrid Chytridiales ITS rDNA Phylogeny Systematics fungal DNA RNA 18S RNA 28S RNA 5.8S electron microscopy fungus molecular analysis new genus new species phylogenetics phylogeny spore taxonomy ultrastructure Argentina article chemistry Chytridiomycetes genetics isolation and purification molecular genetics nucleotide sequence phylogeny polymerase chain reaction transmission electron microscopy ultrastructure Argentina Chytridiomycota DNA, Fungal Microscopy, Electron, Transmission Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 18S RNA, Ribosomal, 28S RNA, Ribosomal, 5.8S algae Chytridiaceae Chytridiales Chytridiomycota Chytridium Chytridium lagenaria Oedogonium capilliforme Olla Phlyctochytrium planicorne Chytridium olla A. Braun, the first described chytrid and an obligate algal parasite, is the type for the genus and thus the foundation of family Chytridiaceae, order Chytridiales, class Chytridiomycetes and phylum Chytridiomycota. Chytridium olla was isolated in coculture with its host, Oedogonium capilliforme. DNA was extracted from the coculture, and 18S, 28S and ITS1-5.8S-ITS2 rDNA were amplified with universal fungal primers. Free swimming zoospores and zoospores in mature sporangia were examined with electron microscopy. Molecular analyses placed C. olla in a clade in Chytridiales with isolates of Chytridium lagenaria and Phlyctochytrium planicorne. Ultrastructural analysis revealed C. olla to have a Group II-type zoospore, previously described for Chytridium lagenaria and Phlyctochytrium planicorne. On the basis of zoospore ultrastructure, family Chytridiaceae is emended to include the type of Chytridium and other species with a Group II-type zoospore, and the new family Chytriomycetaceae is delineated to include members of Chytridiales with a Group I-type zoospore. © 2011 by The Mycological Society of America. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v103_n1_p118_Velez http://hdl.handle.net/20.500.12110/paper_00275514_v103_n1_p118_Velez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
18S rDNA 28S rDNA Chytrid Chytridiales ITS rDNA Phylogeny Systematics fungal DNA RNA 18S RNA 28S RNA 5.8S electron microscopy fungus molecular analysis new genus new species phylogenetics phylogeny spore taxonomy ultrastructure Argentina article chemistry Chytridiomycetes genetics isolation and purification molecular genetics nucleotide sequence phylogeny polymerase chain reaction transmission electron microscopy ultrastructure Argentina Chytridiomycota DNA, Fungal Microscopy, Electron, Transmission Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 18S RNA, Ribosomal, 28S RNA, Ribosomal, 5.8S algae Chytridiaceae Chytridiales Chytridiomycota Chytridium Chytridium lagenaria Oedogonium capilliforme Olla Phlyctochytrium planicorne |
| spellingShingle |
18S rDNA 28S rDNA Chytrid Chytridiales ITS rDNA Phylogeny Systematics fungal DNA RNA 18S RNA 28S RNA 5.8S electron microscopy fungus molecular analysis new genus new species phylogenetics phylogeny spore taxonomy ultrastructure Argentina article chemistry Chytridiomycetes genetics isolation and purification molecular genetics nucleotide sequence phylogeny polymerase chain reaction transmission electron microscopy ultrastructure Argentina Chytridiomycota DNA, Fungal Microscopy, Electron, Transmission Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 18S RNA, Ribosomal, 28S RNA, Ribosomal, 5.8S algae Chytridiaceae Chytridiales Chytridiomycota Chytridium Chytridium lagenaria Oedogonium capilliforme Olla Phlyctochytrium planicorne Molecular phylogenetic and zoospore ultrastructural analyses of Chytridium olla establish the limits of a monophyletic Chytridiales |
| topic_facet |
18S rDNA 28S rDNA Chytrid Chytridiales ITS rDNA Phylogeny Systematics fungal DNA RNA 18S RNA 28S RNA 5.8S electron microscopy fungus molecular analysis new genus new species phylogenetics phylogeny spore taxonomy ultrastructure Argentina article chemistry Chytridiomycetes genetics isolation and purification molecular genetics nucleotide sequence phylogeny polymerase chain reaction transmission electron microscopy ultrastructure Argentina Chytridiomycota DNA, Fungal Microscopy, Electron, Transmission Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 18S RNA, Ribosomal, 28S RNA, Ribosomal, 5.8S algae Chytridiaceae Chytridiales Chytridiomycota Chytridium Chytridium lagenaria Oedogonium capilliforme Olla Phlyctochytrium planicorne |
| description |
Chytridium olla A. Braun, the first described chytrid and an obligate algal parasite, is the type for the genus and thus the foundation of family Chytridiaceae, order Chytridiales, class Chytridiomycetes and phylum Chytridiomycota. Chytridium olla was isolated in coculture with its host, Oedogonium capilliforme. DNA was extracted from the coculture, and 18S, 28S and ITS1-5.8S-ITS2 rDNA were amplified with universal fungal primers. Free swimming zoospores and zoospores in mature sporangia were examined with electron microscopy. Molecular analyses placed C. olla in a clade in Chytridiales with isolates of Chytridium lagenaria and Phlyctochytrium planicorne. Ultrastructural analysis revealed C. olla to have a Group II-type zoospore, previously described for Chytridium lagenaria and Phlyctochytrium planicorne. On the basis of zoospore ultrastructure, family Chytridiaceae is emended to include the type of Chytridium and other species with a Group II-type zoospore, and the new family Chytriomycetaceae is delineated to include members of Chytridiales with a Group I-type zoospore. © 2011 by The Mycological Society of America. |
| title |
Molecular phylogenetic and zoospore ultrastructural analyses of Chytridium olla establish the limits of a monophyletic Chytridiales |
| title_short |
Molecular phylogenetic and zoospore ultrastructural analyses of Chytridium olla establish the limits of a monophyletic Chytridiales |
| title_full |
Molecular phylogenetic and zoospore ultrastructural analyses of Chytridium olla establish the limits of a monophyletic Chytridiales |
| title_fullStr |
Molecular phylogenetic and zoospore ultrastructural analyses of Chytridium olla establish the limits of a monophyletic Chytridiales |
| title_full_unstemmed |
Molecular phylogenetic and zoospore ultrastructural analyses of Chytridium olla establish the limits of a monophyletic Chytridiales |
| title_sort |
molecular phylogenetic and zoospore ultrastructural analyses of chytridium olla establish the limits of a monophyletic chytridiales |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v103_n1_p118_Velez http://hdl.handle.net/20.500.12110/paper_00275514_v103_n1_p118_Velez |
| _version_ |
1768546664546566144 |