The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response

A functional relationship between the neuroendocrine and immune systems has been clearly established. We examined the role of neuroendocrine changes, particularly thyrotropin-releasing hormone (TRH) and prolactin (PRL), during the T cell-dependent immune response. After immunization of rats with she...

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Autores principales:	Perez Castro, Carolina Inés, Páez Pereda, Marcelo
Publicado:	1998
Materias:	Immune modulation Prolactin SRBC T-lymphocytes TRH antisense antisense oligonucleotide protirelin analysis of variance animal antibody production article blood erythrocyte immune system immunology male metabolism neurosecretion rat sheep T lymphocyte Wistar rat Analysis of Variance Animals Antibody Formation Erythrocytes Immune System Male Neurosecretory Systems Oligonucleotides, Antisense Rats Rats, Wistar Sheep T-Lymphocytes Thyrotropin-Releasing Hormone
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v58_n2_p189_Castro http://hdl.handle.net/20.500.12110/paper_00257680_v58_n2_p189_Castro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00257680_v58_n2_p189_Castro
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spelling	paper:paper_00257680_v58_n2_p189_Castro2023-06-08T14:53:30Z The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response Perez Castro, Carolina Inés Páez Pereda, Marcelo Immune modulation Prolactin SRBC T-lymphocytes TRH antisense antisense oligonucleotide protirelin analysis of variance animal antibody production article blood erythrocyte immune system immunology male metabolism neurosecretion rat sheep T lymphocyte Wistar rat Analysis of Variance Animals Antibody Formation Erythrocytes Immune System Male Neurosecretory Systems Oligonucleotides, Antisense Rats Rats, Wistar Sheep T-Lymphocytes Thyrotropin-Releasing Hormone A functional relationship between the neuroendocrine and immune systems has been clearly established. We examined the role of neuroendocrine changes, particularly thyrotropin-releasing hormone (TRH) and prolactin (PRL), during the T cell-dependent immune response. After immunization of rats with sheep red blood cells (SRBC, a T cell-dependent antigen) we observed: a) an increase of hypothalamic TRH mRNA at 4 to 24 h post-immunization (i.e: SRBC vs saline: 4 h, 2.8x), in contrast to the decrease of TRH mRNA observed following treatment with LPS, a T-independent antigen (LPS vs saline: 4 h, 1.6x); b) an increase in pituitary TRH receptor mRNA and plasma PRL levels but no changes in thyroid-stimulating hormone and growth hormone plasma levels. Intracerebroventricular (icv) injection in conscious freely-moving rats of antisense oligonucleotide complementary to rat TRH mRNA resulted in: a) a significant inhibition of specific antibody production [ELISA 7 days: Ig(M+G): TRH sense vs TRH-antisense: 384 ± 27 (n = 11) vs 193 ± 22 (n = 11); p < 0.001] b) an inability to produce the peak in plasma PRL levels in rats immunized with SRBC [(12h post-immunization, TRH-sense vs TRH-antisense: 8.3 ± 1.4 (n = 6) vs 2.2 ± 0.5 (n = 6); p < 0 01]; c) a decrease in hypothalamic TRH mRNA (TRH-sense vs TRH-antisense: 12h, 1.7x). These studies demonstrate that the T-cell antigen needs an early activation of TRH and PRL for an adequate immune response, in contrast to the inhibition induced by a T-cell independent antigen. Fil:Castro, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereda, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v58_n2_p189_Castro http://hdl.handle.net/20.500.12110/paper_00257680_v58_n2_p189_Castro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Immune modulation Prolactin SRBC T-lymphocytes TRH antisense antisense oligonucleotide protirelin analysis of variance animal antibody production article blood erythrocyte immune system immunology male metabolism neurosecretion rat sheep T lymphocyte Wistar rat Analysis of Variance Animals Antibody Formation Erythrocytes Immune System Male Neurosecretory Systems Oligonucleotides, Antisense Rats Rats, Wistar Sheep T-Lymphocytes Thyrotropin-Releasing Hormone
spellingShingle	Immune modulation Prolactin SRBC T-lymphocytes TRH antisense antisense oligonucleotide protirelin analysis of variance animal antibody production article blood erythrocyte immune system immunology male metabolism neurosecretion rat sheep T lymphocyte Wistar rat Analysis of Variance Animals Antibody Formation Erythrocytes Immune System Male Neurosecretory Systems Oligonucleotides, Antisense Rats Rats, Wistar Sheep T-Lymphocytes Thyrotropin-Releasing Hormone Perez Castro, Carolina Inés Páez Pereda, Marcelo The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
topic_facet	Immune modulation Prolactin SRBC T-lymphocytes TRH antisense antisense oligonucleotide protirelin analysis of variance animal antibody production article blood erythrocyte immune system immunology male metabolism neurosecretion rat sheep T lymphocyte Wistar rat Analysis of Variance Animals Antibody Formation Erythrocytes Immune System Male Neurosecretory Systems Oligonucleotides, Antisense Rats Rats, Wistar Sheep T-Lymphocytes Thyrotropin-Releasing Hormone
description	A functional relationship between the neuroendocrine and immune systems has been clearly established. We examined the role of neuroendocrine changes, particularly thyrotropin-releasing hormone (TRH) and prolactin (PRL), during the T cell-dependent immune response. After immunization of rats with sheep red blood cells (SRBC, a T cell-dependent antigen) we observed: a) an increase of hypothalamic TRH mRNA at 4 to 24 h post-immunization (i.e: SRBC vs saline: 4 h, 2.8x), in contrast to the decrease of TRH mRNA observed following treatment with LPS, a T-independent antigen (LPS vs saline: 4 h, 1.6x); b) an increase in pituitary TRH receptor mRNA and plasma PRL levels but no changes in thyroid-stimulating hormone and growth hormone plasma levels. Intracerebroventricular (icv) injection in conscious freely-moving rats of antisense oligonucleotide complementary to rat TRH mRNA resulted in: a) a significant inhibition of specific antibody production [ELISA 7 days: Ig(M+G): TRH sense vs TRH-antisense: 384 ± 27 (n = 11) vs 193 ± 22 (n = 11); p < 0.001] b) an inability to produce the peak in plasma PRL levels in rats immunized with SRBC [(12h post-immunization, TRH-sense vs TRH-antisense: 8.3 ± 1.4 (n = 6) vs 2.2 ± 0.5 (n = 6); p < 0 01]; c) a decrease in hypothalamic TRH mRNA (TRH-sense vs TRH-antisense: 12h, 1.7x). These studies demonstrate that the T-cell antigen needs an early activation of TRH and PRL for an adequate immune response, in contrast to the inhibition induced by a T-cell independent antigen.
author	Perez Castro, Carolina Inés Páez Pereda, Marcelo
author_facet	Perez Castro, Carolina Inés Páez Pereda, Marcelo
author_sort	Perez Castro, Carolina Inés
title	The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
title_short	The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
title_full	The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
title_fullStr	The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
title_full_unstemmed	The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
title_sort	specific neuroendocrine profile is critically involved in an adequate t-dependent immune response
publishDate	1998
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v58_n2_p189_Castro http://hdl.handle.net/20.500.12110/paper_00257680_v58_n2_p189_Castro
work_keys_str_mv	AT perezcastrocarolinaines thespecificneuroendocrineprofileiscriticallyinvolvedinanadequatetdependentimmuneresponse AT paezperedamarcelo thespecificneuroendocrineprofileiscriticallyinvolvedinanadequatetdependentimmuneresponse AT perezcastrocarolinaines specificneuroendocrineprofileiscriticallyinvolvedinanadequatetdependentimmuneresponse AT paezperedamarcelo specificneuroendocrineprofileiscriticallyinvolvedinanadequatetdependentimmuneresponse
_version_	1768545773479264256

The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response

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