Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH)

Long treatments with GnRH agonist are used in patients to suppress the endogenous secretion of gonadotropins, however; these analogs have a direct effect on the ovary. The aim of this work was to study the in vivo effect of the GnRH analogue, leuprolide acetate (LA) on ovarian steroidogenesis and ap...

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Autores principales:	Andreu, Claudia Viviana, Parborell, M.Fernanda A., Tesone, Marta
Publicado:	1997
Materias:	bucladesine chorionic gonadotropin cyclic amp gonadorelin agonist leuprorelin seric gonadotropin antineoplastic hormone agonists and antagonists animal experiment animal tissue apoptosis article controlled study drug effect female nonhuman ovary rat regulatory mechanism steroidogenesis animal corpus luteum follicular phase physiology Sprague Dawley rat Animals Antineoplastic Agents, Hormonal Corpus Luteum Female Follicular Phase Leuprolide Rats Rats, Sprague-Dawley
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n1_p83_Andreu http://hdl.handle.net/20.500.12110/paper_00257680_v57_n1_p83_Andreu
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00257680_v57_n1_p83_Andreu
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spelling	paper:paper_00257680_v57_n1_p83_Andreu2023-06-08T14:53:28Z Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH) Andreu, Claudia Viviana Parborell, M.Fernanda A. Tesone, Marta bucladesine chorionic gonadotropin cyclic amp gonadorelin agonist leuprorelin seric gonadotropin antineoplastic hormone agonists and antagonists animal experiment animal tissue apoptosis article controlled study drug effect female nonhuman ovary rat regulatory mechanism steroidogenesis animal corpus luteum follicular phase physiology Sprague Dawley rat Animals Antineoplastic Agents, Hormonal Corpus Luteum Female Follicular Phase Leuprolide Rats Rats, Sprague-Dawley Long treatments with GnRH agonist are used in patients to suppress the endogenous secretion of gonadotropins, however; these analogs have a direct effect on the ovary. The aim of this work was to study the in vivo effect of the GnRH analogue, leuprolide acetate (LA) on ovarian steroidogenesis and apoptosis mechanisms. LA (1 μg/rat/day) was injected to PMSG/hCG superovulated rats. Corpora lutea were isolated by microdissection and incubated (4/0.5 ml) during 3 h with LH (10 ng/ml) for dibutyryl cAMP (dcAMP 1 mM). Progesterone production was measured observing in LA treated rats a decrease in basal and LH stimulated values (Basal = Control C: 96.6 ± 9.6; LA: 22.9 ± 2.8; LH = C: 145.7 ± 4.9; LA: 23.6 ± 2.0 ng/ml, p < 0,001). In contrast, dcAMP stimulated significantly both groups (C: 153.9 ± 11.8; LA: 83.15 ± 8.2). cAMP production was lower in LA corpora lutea and LH was not able to stimulate them (Basal = C: 7.29 ± 1.6; LA: 1.17 ± 0.6; LH = C: 13.2 ± 0.4; LA: 2.5 ± 0.4 ng/ml, p < 0.01). Corpus luteum of both groups showed similar protein content. On the other hand, taking into account that in ovarian histological slides of LA treated rats we observed more atresic follicles and less corpora lutea, we determined the amount of apoptotic cells. The criterion used was the presence of apoptotic bodies and nuclear chromatin aggregated in dense masses beneath the nuclear envelope. An increase of apoptotic cells in the LA group ovaries was detected. This result was confirmed by immunohistochemical techniques (TUNEL). It was concluded that LA treatment produces in ovarian cells a failure in the LH receptor-adenylate cyclase system and an increase in cellular apoptosis. Fil:Andreu, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n1_p83_Andreu http://hdl.handle.net/20.500.12110/paper_00257680_v57_n1_p83_Andreu
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bucladesine chorionic gonadotropin cyclic amp gonadorelin agonist leuprorelin seric gonadotropin antineoplastic hormone agonists and antagonists animal experiment animal tissue apoptosis article controlled study drug effect female nonhuman ovary rat regulatory mechanism steroidogenesis animal corpus luteum follicular phase physiology Sprague Dawley rat Animals Antineoplastic Agents, Hormonal Corpus Luteum Female Follicular Phase Leuprolide Rats Rats, Sprague-Dawley
spellingShingle	bucladesine chorionic gonadotropin cyclic amp gonadorelin agonist leuprorelin seric gonadotropin antineoplastic hormone agonists and antagonists animal experiment animal tissue apoptosis article controlled study drug effect female nonhuman ovary rat regulatory mechanism steroidogenesis animal corpus luteum follicular phase physiology Sprague Dawley rat Animals Antineoplastic Agents, Hormonal Corpus Luteum Female Follicular Phase Leuprolide Rats Rats, Sprague-Dawley Andreu, Claudia Viviana Parborell, M.Fernanda A. Tesone, Marta Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH)
topic_facet	bucladesine chorionic gonadotropin cyclic amp gonadorelin agonist leuprorelin seric gonadotropin antineoplastic hormone agonists and antagonists animal experiment animal tissue apoptosis article controlled study drug effect female nonhuman ovary rat regulatory mechanism steroidogenesis animal corpus luteum follicular phase physiology Sprague Dawley rat Animals Antineoplastic Agents, Hormonal Corpus Luteum Female Follicular Phase Leuprolide Rats Rats, Sprague-Dawley
description	Long treatments with GnRH agonist are used in patients to suppress the endogenous secretion of gonadotropins, however; these analogs have a direct effect on the ovary. The aim of this work was to study the in vivo effect of the GnRH analogue, leuprolide acetate (LA) on ovarian steroidogenesis and apoptosis mechanisms. LA (1 μg/rat/day) was injected to PMSG/hCG superovulated rats. Corpora lutea were isolated by microdissection and incubated (4/0.5 ml) during 3 h with LH (10 ng/ml) for dibutyryl cAMP (dcAMP 1 mM). Progesterone production was measured observing in LA treated rats a decrease in basal and LH stimulated values (Basal = Control C: 96.6 ± 9.6; LA: 22.9 ± 2.8; LH = C: 145.7 ± 4.9; LA: 23.6 ± 2.0 ng/ml, p < 0,001). In contrast, dcAMP stimulated significantly both groups (C: 153.9 ± 11.8; LA: 83.15 ± 8.2). cAMP production was lower in LA corpora lutea and LH was not able to stimulate them (Basal = C: 7.29 ± 1.6; LA: 1.17 ± 0.6; LH = C: 13.2 ± 0.4; LA: 2.5 ± 0.4 ng/ml, p < 0.01). Corpus luteum of both groups showed similar protein content. On the other hand, taking into account that in ovarian histological slides of LA treated rats we observed more atresic follicles and less corpora lutea, we determined the amount of apoptotic cells. The criterion used was the presence of apoptotic bodies and nuclear chromatin aggregated in dense masses beneath the nuclear envelope. An increase of apoptotic cells in the LA group ovaries was detected. This result was confirmed by immunohistochemical techniques (TUNEL). It was concluded that LA treatment produces in ovarian cells a failure in the LH receptor-adenylate cyclase system and an increase in cellular apoptosis.
author	Andreu, Claudia Viviana Parborell, M.Fernanda A. Tesone, Marta
author_facet	Andreu, Claudia Viviana Parborell, M.Fernanda A. Tesone, Marta
author_sort	Andreu, Claudia Viviana
title	Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH)
title_short	Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH)
title_full	Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH)
title_fullStr	Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH)
title_full_unstemmed	Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH)
title_sort	regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (gnrh)
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n1_p83_Andreu http://hdl.handle.net/20.500.12110/paper_00257680_v57_n1_p83_Andreu
work_keys_str_mv	AT andreuclaudiaviviana regulationoffollicularluteinizationbyanagonistofgonadotropinreleasinghormonegnrh AT parborellmfernandaa regulationoffollicularluteinizationbyanagonistofgonadotropinreleasinghormonegnrh AT tesonemarta regulationoffollicularluteinizationbyanagonistofgonadotropinreleasinghormonegnrh
_version_	1768541694316249088

Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH)

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