Estradiol increases glucocorticoid binding and glucocorticoid induction of ornithine decarboxylase in the rat spinal cord

Previous results demonstrated that estradiol (E2) treatment of ovariectomized-adrenalectomized (OVX-ADX) rats increased glucocorticoid (GC) binding in brain regions. The experimental protocol was extended to the spinal cord, a GC target tissue in which ornithine decarboxylase (ODC) is markedly induc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Publicado:	1993
Materias:	dexamethasone estradiol estradiol receptor glucocorticoid receptor ornithine decarboxylase radioligand adrenalectomy animal experiment animal tissue article binding site controlled study enzyme induction female nonhuman ovariectomy priority journal rat spinal cord spinal cord dorsal horn spinal cord ventral horn subcutaneous drug administration Adrenalectomy Analysis of Variance Animal Enzyme Induction Estradiol Female Glucocorticoids Ornithine Decarboxylase Ovariectomy Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Spinal Cord Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Animalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v52_n7_p677_Ferrini http://hdl.handle.net/20.500.12110/paper_00243205_v52_n7_p677_Ferrini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00243205_v52_n7_p677_Ferrini
record_format	dspace
spelling	paper:paper_00243205_v52_n7_p677_Ferrini2023-06-08T14:52:00Z Estradiol increases glucocorticoid binding and glucocorticoid induction of ornithine decarboxylase in the rat spinal cord dexamethasone estradiol estradiol receptor glucocorticoid receptor ornithine decarboxylase radioligand adrenalectomy animal experiment animal tissue article binding site controlled study enzyme induction female nonhuman ovariectomy priority journal rat spinal cord spinal cord dorsal horn spinal cord ventral horn subcutaneous drug administration Adrenalectomy Analysis of Variance Animal Enzyme Induction Estradiol Female Glucocorticoids Ornithine Decarboxylase Ovariectomy Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Spinal Cord Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Animalia Previous results demonstrated that estradiol (E2) treatment of ovariectomized-adrenalectomized (OVX-ADX) rats increased glucocorticoid (GC) binding in brain regions. The experimental protocol was extended to the spinal cord, a GC target tissue in which ornithine decarboxylase (ODC) is markedly induced by GC treatment. First, we measured GC binding to type I and type II receptors in ventral horn, dorsal horn and lateral funiculus of OVX-ADX rats treated during 4 days with E2 or vehicle. In E2-treated rats, type II receptors increased solely in dorsal horn, whereas type I sites remained unchanged. Second, in a group of OVX-ADX rats receiving dexamethasone (DEX), pretreatment with E2 superinduced ODC in ventral horn and lateral funiculus, but not in dorsal horn. Third, we found that the dorsal horn was relatively enriched in E2 receptors compared to other areas. Therefore, E2 stimulation of GC binding to type II sites may be mediated through E2 receptors localized in the dorsal horn. We suggest that combined treatment with E2 and DEX employs a transsynaptic mechanism for ODC induction at the ventral horn and lateral funiculus, with hormonal interaction taking place at the dorsal horn level. © 1993. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v52_n7_p677_Ferrini http://hdl.handle.net/20.500.12110/paper_00243205_v52_n7_p677_Ferrini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	dexamethasone estradiol estradiol receptor glucocorticoid receptor ornithine decarboxylase radioligand adrenalectomy animal experiment animal tissue article binding site controlled study enzyme induction female nonhuman ovariectomy priority journal rat spinal cord spinal cord dorsal horn spinal cord ventral horn subcutaneous drug administration Adrenalectomy Analysis of Variance Animal Enzyme Induction Estradiol Female Glucocorticoids Ornithine Decarboxylase Ovariectomy Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Spinal Cord Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Animalia
spellingShingle	dexamethasone estradiol estradiol receptor glucocorticoid receptor ornithine decarboxylase radioligand adrenalectomy animal experiment animal tissue article binding site controlled study enzyme induction female nonhuman ovariectomy priority journal rat spinal cord spinal cord dorsal horn spinal cord ventral horn subcutaneous drug administration Adrenalectomy Analysis of Variance Animal Enzyme Induction Estradiol Female Glucocorticoids Ornithine Decarboxylase Ovariectomy Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Spinal Cord Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Animalia Estradiol increases glucocorticoid binding and glucocorticoid induction of ornithine decarboxylase in the rat spinal cord
topic_facet	dexamethasone estradiol estradiol receptor glucocorticoid receptor ornithine decarboxylase radioligand adrenalectomy animal experiment animal tissue article binding site controlled study enzyme induction female nonhuman ovariectomy priority journal rat spinal cord spinal cord dorsal horn spinal cord ventral horn subcutaneous drug administration Adrenalectomy Analysis of Variance Animal Enzyme Induction Estradiol Female Glucocorticoids Ornithine Decarboxylase Ovariectomy Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Spinal Cord Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Animalia
description	Previous results demonstrated that estradiol (E2) treatment of ovariectomized-adrenalectomized (OVX-ADX) rats increased glucocorticoid (GC) binding in brain regions. The experimental protocol was extended to the spinal cord, a GC target tissue in which ornithine decarboxylase (ODC) is markedly induced by GC treatment. First, we measured GC binding to type I and type II receptors in ventral horn, dorsal horn and lateral funiculus of OVX-ADX rats treated during 4 days with E2 or vehicle. In E2-treated rats, type II receptors increased solely in dorsal horn, whereas type I sites remained unchanged. Second, in a group of OVX-ADX rats receiving dexamethasone (DEX), pretreatment with E2 superinduced ODC in ventral horn and lateral funiculus, but not in dorsal horn. Third, we found that the dorsal horn was relatively enriched in E2 receptors compared to other areas. Therefore, E2 stimulation of GC binding to type II sites may be mediated through E2 receptors localized in the dorsal horn. We suggest that combined treatment with E2 and DEX employs a transsynaptic mechanism for ODC induction at the ventral horn and lateral funiculus, with hormonal interaction taking place at the dorsal horn level. © 1993.
title	Estradiol increases glucocorticoid binding and glucocorticoid induction of ornithine decarboxylase in the rat spinal cord
title_short	Estradiol increases glucocorticoid binding and glucocorticoid induction of ornithine decarboxylase in the rat spinal cord
title_full	Estradiol increases glucocorticoid binding and glucocorticoid induction of ornithine decarboxylase in the rat spinal cord
title_fullStr	Estradiol increases glucocorticoid binding and glucocorticoid induction of ornithine decarboxylase in the rat spinal cord
title_full_unstemmed	Estradiol increases glucocorticoid binding and glucocorticoid induction of ornithine decarboxylase in the rat spinal cord
title_sort	estradiol increases glucocorticoid binding and glucocorticoid induction of ornithine decarboxylase in the rat spinal cord
publishDate	1993
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v52_n7_p677_Ferrini http://hdl.handle.net/20.500.12110/paper_00243205_v52_n7_p677_Ferrini
_version_	1768541552525705216

Estradiol increases glucocorticoid binding and glucocorticoid induction of ornithine decarboxylase in the rat spinal cord

Ejemplares similares