High correlation between prolactinemia, 125-I hLH binding and progesterone secretion by an experimental luteoma
Autoimplantation of an ovary, containing fresh corpora lutea, into the spleen of an ovariectomized rat is followed by strong luteinization and size increase of the grafted gonad. Thus, large amounts of luteal tissue for biochemical studies, and their histological controls are available. Furthermore,...
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1984
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v35_n23_p2345_Lux http://hdl.handle.net/20.500.12110/paper_00243205_v35_n23_p2345_Lux |
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paper:paper_00243205_v35_n23_p2345_Lux2023-06-08T14:51:58Z High correlation between prolactinemia, 125-I hLH binding and progesterone secretion by an experimental luteoma luteinizing hormone receptor progesterone radioisotope animal cell corpus luteum endocrine system female genital system luteinization luteinizing hormone i 125 nonhuman ovary graft priority journal prolactin blood level rat Animal Corpus Luteum Female Kinetics Luteinizing Hormone Ovary Progesterone Prolactin Rats Rats, Inbred Strains Receptors, Cell Surface Receptors, LH Support, Non-U.S. Gov't Transplantation, Autologous Autoimplantation of an ovary, containing fresh corpora lutea, into the spleen of an ovariectomized rat is followed by strong luteinization and size increase of the grafted gonad. Thus, large amounts of luteal tissue for biochemical studies, and their histological controls are available. Furthermore, progesterone secretion can be easily determined in samples collected from the portal vein. Since prolactin has been implicated in the control of luteal tissue, the role of this hormone on hLH binding and progesterone secretion was determined. Different levels of endogenous serum prolactin were achieved by pharmacological treatments with neurotropic agents. Scatchard plots of 125-I hLH binding data derived from luteoma particulate fractions revealed the presence of one type of binding site with high affinity. At the same time as binding increased, prolactinemia augmented, with a high correlation (R:0.99) between prolactinemia and LH binding. Moreover, progesterone secreted by the luteoma increased as LH binding sites augmented (R:0,97). It is concluded that a high correlation between prolactinemia and LH binding, as well as between this last parameter and progesterone output exists in the experimental luteoma. © 1984. 1984 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v35_n23_p2345_Lux http://hdl.handle.net/20.500.12110/paper_00243205_v35_n23_p2345_Lux |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
luteinizing hormone receptor progesterone radioisotope animal cell corpus luteum endocrine system female genital system luteinization luteinizing hormone i 125 nonhuman ovary graft priority journal prolactin blood level rat Animal Corpus Luteum Female Kinetics Luteinizing Hormone Ovary Progesterone Prolactin Rats Rats, Inbred Strains Receptors, Cell Surface Receptors, LH Support, Non-U.S. Gov't Transplantation, Autologous |
| spellingShingle |
luteinizing hormone receptor progesterone radioisotope animal cell corpus luteum endocrine system female genital system luteinization luteinizing hormone i 125 nonhuman ovary graft priority journal prolactin blood level rat Animal Corpus Luteum Female Kinetics Luteinizing Hormone Ovary Progesterone Prolactin Rats Rats, Inbred Strains Receptors, Cell Surface Receptors, LH Support, Non-U.S. Gov't Transplantation, Autologous High correlation between prolactinemia, 125-I hLH binding and progesterone secretion by an experimental luteoma |
| topic_facet |
luteinizing hormone receptor progesterone radioisotope animal cell corpus luteum endocrine system female genital system luteinization luteinizing hormone i 125 nonhuman ovary graft priority journal prolactin blood level rat Animal Corpus Luteum Female Kinetics Luteinizing Hormone Ovary Progesterone Prolactin Rats Rats, Inbred Strains Receptors, Cell Surface Receptors, LH Support, Non-U.S. Gov't Transplantation, Autologous |
| description |
Autoimplantation of an ovary, containing fresh corpora lutea, into the spleen of an ovariectomized rat is followed by strong luteinization and size increase of the grafted gonad. Thus, large amounts of luteal tissue for biochemical studies, and their histological controls are available. Furthermore, progesterone secretion can be easily determined in samples collected from the portal vein. Since prolactin has been implicated in the control of luteal tissue, the role of this hormone on hLH binding and progesterone secretion was determined. Different levels of endogenous serum prolactin were achieved by pharmacological treatments with neurotropic agents. Scatchard plots of 125-I hLH binding data derived from luteoma particulate fractions revealed the presence of one type of binding site with high affinity. At the same time as binding increased, prolactinemia augmented, with a high correlation (R:0.99) between prolactinemia and LH binding. Moreover, progesterone secreted by the luteoma increased as LH binding sites augmented (R:0,97). It is concluded that a high correlation between prolactinemia and LH binding, as well as between this last parameter and progesterone output exists in the experimental luteoma. © 1984. |
| title |
High correlation between prolactinemia, 125-I hLH binding and progesterone secretion by an experimental luteoma |
| title_short |
High correlation between prolactinemia, 125-I hLH binding and progesterone secretion by an experimental luteoma |
| title_full |
High correlation between prolactinemia, 125-I hLH binding and progesterone secretion by an experimental luteoma |
| title_fullStr |
High correlation between prolactinemia, 125-I hLH binding and progesterone secretion by an experimental luteoma |
| title_full_unstemmed |
High correlation between prolactinemia, 125-I hLH binding and progesterone secretion by an experimental luteoma |
| title_sort |
high correlation between prolactinemia, 125-i hlh binding and progesterone secretion by an experimental luteoma |
| publishDate |
1984 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v35_n23_p2345_Lux http://hdl.handle.net/20.500.12110/paper_00243205_v35_n23_p2345_Lux |
| _version_ |
1768545865458253824 |